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The map label for this gene is rlmN

Identifier: 126208740

GI number: 126208740

Start: 1464652

End: 1465833

Strand: Reverse

Name: rlmN

Synonym: APL_1274

Alternate gene names: 126208740

Gene position: 1465833-1464652 (Counterclockwise)

Preceding gene: 126208743

Following gene: 126208739

Centisome position: 64.45

GC content: 42.72

Gene sequence:

>1182_bases
ATGTCAGAACAAACCCAAACTTGTGCGAGCGAAATTCAAGCAACGAATGTCGCAGTACAGCACCCTAAATCAGAAAAAAT
TAACCTGATGAACTTAACTCGCAAGGAAATGCGTGAGTTATTTGCCGAAATGGGCGAAAAACCGTTTCGTGCCGACCAAT
TAATGAAATGGATTTACCATTTCGGTGAAGACAATTTCGATAATATGAGCAATATCAATAAAGTATTGCGTGAAAAATTA
AAACAAATTGCAGAAATCAAAGCGCCGGAAGTCTCTGTCGAACAGCGTTCAAGCGACGGCACAATCAAATGGGCGATGCA
GGTGGGTGATCAGCAAATCGAAACCGTATATATCCCGGAAGATGACCGTGCGACTTTATGCGTTTCTTCTCAAGTTGGTT
GTGCGTTAGCCTGTAAATTCTGTTCAACCGCTCAACAAGGTTTTAACCGTAACTTAACCGTTTCCGAGATTATCGGTCAG
GTATGGCGAGCTTCGAAAATCATTGGTAACTTCGGGATAACCGGTGTGCGTCCGATTACCAACGTGGTAATGATGGGGAT
GGGCGAACCGTTACTCAACTTAAATAACGTGATTCCGGCAATGGAAATTATGTTGGATGACTTTGCATACGGTTTATCAA
AACGCCGTGTAACACTTTCAACGGCAGGTGTTGTGCCGGCGCTTGATATTATGCGTGAAAAAATTGACGTAGCATTAGCG
ATTTCATTGCATGCACCGAATGATGAATTGCGTGATGAAATTATGCCGATTAACAAAAAGTATAATATCAAAATGCTGAT
GGATTCGGTGCATAAATACCTTGAAGTATCGAATGCGAACCACGGTAAAGTGACGATTGAATACGTGTTATTAGATCACG
TTAATGACGGCACGGAACACGCGCATCAGTTAGCGGAAGTCTTAAAAAATACCCCATGTAAGATCAACCTTATTCCATGG
AACCCTTTCCCGGAAGCACCTTACGGTAAGAGCTCAAATAGCCGAGTGGATCGTTTCCAAAAAACGTTAATGGAATACGG
TTTTACGGTTATTGTACGCAAAACTCGCGGTGATGATATTGATGCCGCTTGCGGTCAGCTTGCCGGTGATGTCATCGACC
GAACCAAACGTACCATGGAAAAACGTAAGTTTGGCAAAGGTATTGCGGTACAAAACCATTAA

Upstream 100 bases:

>100_bases
ATACTGATTGACGAGTAAAATTTGCGGTCTTTTTTATTTTATCAACCTGCAAAACTAATCCTTTTGCCGTAAACCGTGTA
ACAACGAGGCTTTTACTCTC

Downstream 100 bases:

>100_bases
CTAATTTCTCCCTTCGCAACTAAGTGAGGGGAGTATATCTTGAGGAGAACAATATGAACCTTGCAAAATTTTTGCGAAAT
TTGACCGCTTGTATGCTGTT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSEQTQTCASEIQATNVAVQHPKSEKINLMNLTRKEMRELFAEMGEKPFRADQLMKWIYHFGEDNFDNMSNINKVLREKL
KQIAEIKAPEVSVEQRSSDGTIKWAMQVGDQQIETVYIPEDDRATLCVSSQVGCALACKFCSTAQQGFNRNLTVSEIIGQ
VWRASKIIGNFGITGVRPITNVVMMGMGEPLLNLNNVIPAMEIMLDDFAYGLSKRRVTLSTAGVVPALDIMREKIDVALA
ISLHAPNDELRDEIMPINKKYNIKMLMDSVHKYLEVSNANHGKVTIEYVLLDHVNDGTEHAHQLAEVLKNTPCKINLIPW
NPFPEAPYGKSSNSRVDRFQKTLMEYGFTVIVRKTRGDDIDAACGQLAGDVIDRTKRTMEKRKFGKGIAVQNH

Sequences:

>Translated_393_residues
MSEQTQTCASEIQATNVAVQHPKSEKINLMNLTRKEMRELFAEMGEKPFRADQLMKWIYHFGEDNFDNMSNINKVLREKL
KQIAEIKAPEVSVEQRSSDGTIKWAMQVGDQQIETVYIPEDDRATLCVSSQVGCALACKFCSTAQQGFNRNLTVSEIIGQ
VWRASKIIGNFGITGVRPITNVVMMGMGEPLLNLNNVIPAMEIMLDDFAYGLSKRRVTLSTAGVVPALDIMREKIDVALA
ISLHAPNDELRDEIMPINKKYNIKMLMDSVHKYLEVSNANHGKVTIEYVLLDHVNDGTEHAHQLAEVLKNTPCKINLIPW
NPFPEAPYGKSSNSRVDRFQKTLMEYGFTVIVRKTRGDDIDAACGQLAGDVIDRTKRTMEKRKFGKGIAVQNH
>Mature_392_residues
SEQTQTCASEIQATNVAVQHPKSEKINLMNLTRKEMRELFAEMGEKPFRADQLMKWIYHFGEDNFDNMSNINKVLREKLK
QIAEIKAPEVSVEQRSSDGTIKWAMQVGDQQIETVYIPEDDRATLCVSSQVGCALACKFCSTAQQGFNRNLTVSEIIGQV
WRASKIIGNFGITGVRPITNVVMMGMGEPLLNLNNVIPAMEIMLDDFAYGLSKRRVTLSTAGVVPALDIMREKIDVALAI
SLHAPNDELRDEIMPINKKYNIKMLMDSVHKYLEVSNANHGKVTIEYVLLDHVNDGTEHAHQLAEVLKNTPCKINLIPWN
PFPEAPYGKSSNSRVDRFQKTLMEYGFTVIVRKTRGDDIDAACGQLAGDVIDRTKRTMEKRKFGKGIAVQNH

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=369, Percent_Identity=78.5907859078591, Blast_Score=629, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_ACTP2 (A3N1S4)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053965.1
- ProteinModelPortal:   A3N1S4
- STRING:   A3N1S4
- GeneID:   4850169
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1274
- eggNOG:   COG0820
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- PhylomeDB:   A3N1S4
- ProtClustDB:   PRK11194
- BioCyc:   APLE416269:APL_1274-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 44204; Mature: 44072

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQTQTCASEIQATNVAVQHPKSEKINLMNLTRKEMRELFAEMGEKPFRADQLMKWIYH
CCCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FGEDNFDNMSNINKVLREKLKQIAEIKAPEVSVEQRSSDGTIKWAMQVGDQQIETVYIPE
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEEECCCCEEEEEEECC
DDRATLCVSSQVGCALACKFCSTAQQGFNRNLTVSEIIGQVWRASKIIGNFGITGVRPIT
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
NVVMMGMGEPLLNLNNVIPAMEIMLDDFAYGLSKRRVTLSTAGVVPALDIMREKIDVALA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCEEEE
ISLHAPNDELRDEIMPINKKYNIKMLMDSVHKYLEVSNANHGKVTIEYVLLDHVNDGTEH
EEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHH
AHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQKTLMEYGFTVIVRKTRGDDI
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCH
DAACGQLAGDVIDRTKRTMEKRKFGKGIAVQNH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SEQTQTCASEIQATNVAVQHPKSEKINLMNLTRKEMRELFAEMGEKPFRADQLMKWIYH
CCHHHHHHHHHHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FGEDNFDNMSNINKVLREKLKQIAEIKAPEVSVEQRSSDGTIKWAMQVGDQQIETVYIPE
HCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCEEEEEECCCCEEEEEEECC
DDRATLCVSSQVGCALACKFCSTAQQGFNRNLTVSEIIGQVWRASKIIGNFGITGVRPIT
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHH
NVVMMGMGEPLLNLNNVIPAMEIMLDDFAYGLSKRRVTLSTAGVVPALDIMREKIDVALA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCEEEE
ISLHAPNDELRDEIMPINKKYNIKMLMDSVHKYLEVSNANHGKVTIEYVLLDHVNDGTEH
EEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCHHH
AHQLAEVLKNTPCKINLIPWNPFPEAPYGKSSNSRVDRFQKTLMEYGFTVIVRKTRGDDI
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCH
DAACGQLAGDVIDRTKRTMEKRKFGKGIAVQNH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA