Click here to switch to the map view.

The map label for this gene is namA [H]

Identifier: 126208659

GI number: 126208659

Start: 1369523

End: 1370584

Strand: Direct

Name: namA [H]

Synonym: APL_1191

Alternate gene names: 126208659

Gene position: 1369523-1370584 (Clockwise)

Preceding gene: 126208657

Following gene: 126208660

Centisome position: 60.21

GC content: 45.29

Gene sequence:

>1062_bases
ATGGCAAAATTTAGATATTTACAAACACCTTTCAAGCTCAAAAATCTTGAACTTAAAAACCGTATCGTGATGCCGCCGAT
GTGCCAATATTCGGCAACAGACGGTGTGCCGAACGATTGGCATTTTGTGCATTACACCGCTCGTGCGATTGGTGGTGTCG
GCTTAATTATTGTGGAGATGACCAACGTTGCACCAAACGGTCGTATTACACCTAAGTGTTTAGGGCTATGGAATGATGAA
CAACGTGATGCGTTTAAACCGATTGTGGATTCAGTGCATAAATACGGCAGTAAAATTGTGGTGCAAATCGGTCACGCCGG
TCGTAAAGCACAAGATTGCGATGATGTGGTTGCCCCTTCAGCAATTCATTACGGTGAATTGGATTATGCAGGTCAGAATT
TAAAAACACCTCGTGAATTAAGCAAAGCGGAAATTCAGGAAGTGATTCAATCTTACCAAGATGCGGTAAAACGTGCAGTG
GAAGCCGGTTTTGATGCAATTGAAATTCACGGCGCACACGGTTATTTAATTCACCAATTCTCATCACCGAAATCAAATCA
ACGTAGCGATGAATACGGTCAAGATCGCTTCTTATTCGGTGAGCAAGTAATTAAAGCCGCTAAAGCGGTAATGCCGGCAG
ATATGCCGTTGATCGTGCGTATTTCGGCACAAGAATTCGGTGCGAACGGCTTTGATGCGGATTACGGTGTGGAAATGGCA
AGACGCTTTGCGGCAGCCGGTGCGGATTGTATGCACGTAAGTGCCGGCGGTGACGGTGTGTTAGCGGAAGGTAAACATCC
GCCATTTAATGCCGGCTACCAAGTGTATCTTGCTCGTGCGGTAAAACAAGCGACCGGTTTACCTACTATTGCTGTCGGTA
TGTTAGACGATGCGAATGTGGTTGATCATGTGTTATCAATCGGCGATGCGGATTTAGTAGCGGTTGGACGTGCGTTATTA
CGTGACCCGCAATGGGTATTAAATGTACAATATGCACAAAATGCGACAAATAGCAGTGGTGTTGAGTTTGTGCCGGCACA
ATATCAACGTGGTTATTTCTAA

Upstream 100 bases:

>100_bases
TATCACGCATATTTATATTTAGTATAAAGAATAGTGATTAGATTTTATATCTTCAGCCGATTAAAATCCATTCGTTTTTT
AGACATGAAGGATTAGACCA

Downstream 100 bases:

>100_bases
TTAACGTCTATACAAGCGGTGTAAAAAGAAGCATTTTTTACACCGCTTTTTTTTATTTATCACACTTGGATGTATCACAC
TATGCAACTTTCTATTTTAA

Product: NADPH dehydrogenase

Products: NA

Alternate protein names: Xenobiotic reductase [H]

Number of amino acids: Translated: 353; Mature: 352

Protein sequence:

>353_residues
MAKFRYLQTPFKLKNLELKNRIVMPPMCQYSATDGVPNDWHFVHYTARAIGGVGLIIVEMTNVAPNGRITPKCLGLWNDE
QRDAFKPIVDSVHKYGSKIVVQIGHAGRKAQDCDDVVAPSAIHYGELDYAGQNLKTPRELSKAEIQEVIQSYQDAVKRAV
EAGFDAIEIHGAHGYLIHQFSSPKSNQRSDEYGQDRFLFGEQVIKAAKAVMPADMPLIVRISAQEFGANGFDADYGVEMA
RRFAAAGADCMHVSAGGDGVLAEGKHPPFNAGYQVYLARAVKQATGLPTIAVGMLDDANVVDHVLSIGDADLVAVGRALL
RDPQWVLNVQYAQNATNSSGVEFVPAQYQRGYF

Sequences:

>Translated_353_residues
MAKFRYLQTPFKLKNLELKNRIVMPPMCQYSATDGVPNDWHFVHYTARAIGGVGLIIVEMTNVAPNGRITPKCLGLWNDE
QRDAFKPIVDSVHKYGSKIVVQIGHAGRKAQDCDDVVAPSAIHYGELDYAGQNLKTPRELSKAEIQEVIQSYQDAVKRAV
EAGFDAIEIHGAHGYLIHQFSSPKSNQRSDEYGQDRFLFGEQVIKAAKAVMPADMPLIVRISAQEFGANGFDADYGVEMA
RRFAAAGADCMHVSAGGDGVLAEGKHPPFNAGYQVYLARAVKQATGLPTIAVGMLDDANVVDHVLSIGDADLVAVGRALL
RDPQWVLNVQYAQNATNSSGVEFVPAQYQRGYF
>Mature_352_residues
AKFRYLQTPFKLKNLELKNRIVMPPMCQYSATDGVPNDWHFVHYTARAIGGVGLIIVEMTNVAPNGRITPKCLGLWNDEQ
RDAFKPIVDSVHKYGSKIVVQIGHAGRKAQDCDDVVAPSAIHYGELDYAGQNLKTPRELSKAEIQEVIQSYQDAVKRAVE
AGFDAIEIHGAHGYLIHQFSSPKSNQRSDEYGQDRFLFGEQVIKAAKAVMPADMPLIVRISAQEFGANGFDADYGVEMAR
RFAAAGADCMHVSAGGDGVLAEGKHPPFNAGYQVYLARAVKQATGLPTIAVGMLDDANVVDHVLSIGDADLVAVGRALLR
DPQWVLNVQYAQNATNSSGVEFVPAQYQRGYF

Specific function: Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=337, Percent_Identity=31.1572700296736, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787939, Length=202, Percent_Identity=34.6534653465347, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17565138, Length=236, Percent_Identity=28.8135593220339, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=243, Percent_Identity=28.3950617283951, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17559804, Length=243, Percent_Identity=29.2181069958848, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=265, Percent_Identity=26.7924528301887, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=239, Percent_Identity=29.7071129707113, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=346, Percent_Identity=24.5664739884393, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17540738, Length=258, Percent_Identity=26.7441860465116, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=217, Percent_Identity=33.6405529953917, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321973, Length=217, Percent_Identity=32.258064516129, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: =1.6.99.1 [H]

Molecular weight: Translated: 38611; Mature: 38480

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKFRYLQTPFKLKNLELKNRIVMPPMCQYSATDGVPNDWHFVHYTARAIGGVGLIIVEM
CCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCEEEEEEE
TNVAPNGRITPKCLGLWNDEQRDAFKPIVDSVHKYGSKIVVQIGHAGRKAQDCDDVVAPS
ECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCC
AIHYGELDYAGQNLKTPRELSKAEIQEVIQSYQDAVKRAVEAGFDAIEIHGAHGYLIHQF
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEC
SSPKSNQRSDEYGQDRFLFGEQVIKAAKAVMPADMPLIVRISAQEFGANGFDADYGVEMA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCHHHHHHH
RRFAAAGADCMHVSAGGDGVLAEGKHPPFNAGYQVYLARAVKQATGLPTIAVGMLDDANV
HHHHHCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHH
VDHVLSIGDADLVAVGRALLRDPQWVLNVQYAQNATNSSGVEFVPAQYQRGYF
HHHHHHCCCHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCEEECHHHCCCCC
>Mature Secondary Structure 
AKFRYLQTPFKLKNLELKNRIVMPPMCQYSATDGVPNDWHFVHYTARAIGGVGLIIVEM
CCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHCCCEEEEEEE
TNVAPNGRITPKCLGLWNDEQRDAFKPIVDSVHKYGSKIVVQIGHAGRKAQDCDDVVAPS
ECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCC
AIHYGELDYAGQNLKTPRELSKAEIQEVIQSYQDAVKRAVEAGFDAIEIHGAHGYLIHQF
CEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEC
SSPKSNQRSDEYGQDRFLFGEQVIKAAKAVMPADMPLIVRISAQEFGANGFDADYGVEMA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCCHHHHHHH
RRFAAAGADCMHVSAGGDGVLAEGKHPPFNAGYQVYLARAVKQATGLPTIAVGMLDDANV
HHHHHCCCCEEEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHH
VDHVLSIGDADLVAVGRALLRDPQWVLNVQYAQNATNSSGVEFVPAQYQRGYF
HHHHHHCCCHHHHHHHHHHHCCCCCEEEEEHHCCCCCCCCCEEECHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]