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The map label for this gene is trpG [H]

Identifier: 126208632

GI number: 126208632

Start: 1341812

End: 1342399

Strand: Direct

Name: trpG [H]

Synonym: APL_1164

Alternate gene names: 126208632

Gene position: 1341812-1342399 (Clockwise)

Preceding gene: 126208631

Following gene: 126208633

Centisome position: 58.99

GC content: 41.84

Gene sequence:

>588_bases
ATGGCACATATTCTTTTTGTTGATAATTTTGATTCGTTTACCTACAACCTTGTCGATCAATTTCGTGAATTAGGTCATCA
AGTGACGATTTTTCGTAACGATTACCCATTAGAAGACTTTCTAAACAAAGCACAAACTACCGAAAATTGTATTGTGGCAC
TTTCGCCCGGCCCCGGTATACCGGCAGAAGCCGGCAATATGTTAGCAATCATTAAACGACTCAAAGGTGTTGTACCGATG
ATTGGCATTTGTTTGGGGCATCAAGCAATTATCGAAGCGTTAGGCGGACGTATCGTGCATACCGGTACCGTTTTACACGG
CAAGGTGTCAAAAATTGAACACGATAACCAAGCAATGTTCCACGGTATTAATAACCCGATGCCGGTTGCCCGTTACCATT
CACTAATGGGTGATAATCTGCCAGAGGAGTTAGAAATTAATGCTCGCTTCGATAATATCGTGATGGCGATTCGCCACAAA
CAATTACCGATTTGCGGCTTTCAATTTCACCCAGAATCAATCCTAACCGTAGAAGGCACTAAATTATTAAAACAATCGGT
CGAATGGCTGCTTTCCACACAACATTAA

Upstream 100 bases:

>100_bases
GATTCGGATCCGCAATCGGAAGCGGATGAAACGCGTCACAAAGCGAAAGCCGTATTAAAAGCAATCGCACAAGTTAATAC
CAAATAAGGACGGATAAGCT

Downstream 100 bases:

>100_bases
GCAAAAAAATTTAGAAAAATAACCGCTTATCAGTATAACGAAAACAGGATAACACTATGCAACTCGACAACATTCTTAGC
CAATTATTTGAGAACCAAGC

Product: anthranilate synthase component II

Products: NA

Alternate protein names: Glutamine amido-transferase [H]

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MAHILFVDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLNKAQTTENCIVALSPGPGIPAEAGNMLAIIKRLKGVVPM
IGICLGHQAIIEALGGRIVHTGTVLHGKVSKIEHDNQAMFHGINNPMPVARYHSLMGDNLPEELEINARFDNIVMAIRHK
QLPICGFQFHPESILTVEGTKLLKQSVEWLLSTQH

Sequences:

>Translated_195_residues
MAHILFVDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLNKAQTTENCIVALSPGPGIPAEAGNMLAIIKRLKGVVPM
IGICLGHQAIIEALGGRIVHTGTVLHGKVSKIEHDNQAMFHGINNPMPVARYHSLMGDNLPEELEINARFDNIVMAIRHK
QLPICGFQFHPESILTVEGTKLLKQSVEWLLSTQH
>Mature_194_residues
AHILFVDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLNKAQTTENCIVALSPGPGIPAEAGNMLAIIKRLKGVVPMI
GICLGHQAIIEALGGRIVHTGTVLHGKVSKIEHDNQAMFHGINNPMPVARYHSLMGDNLPEELEINARFDNIVMAIRHKQ
LPICGFQFHPESILTVEGTKLLKQSVEWLLSTQH

Specific function: Tryptophan biosynthesis; first step. Tryptophan biosynthesis; second step. [C]

COG id: COG0512

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787517, Length=189, Percent_Identity=57.6719576719577, Blast_Score=246, Evalue=1e-66,
Organism=Escherichia coli, GI1789760, Length=183, Percent_Identity=43.1693989071038, Blast_Score=156, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322638, Length=189, Percent_Identity=38.0952380952381, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6324361, Length=201, Percent_Identity=27.8606965174129, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: 945 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR006221 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 21802; Mature: 21670

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHILFVDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLNKAQTTENCIVALSPGPGI
CEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCC
PAEAGNMLAIIKRLKGVVPMIGICLGHQAIIEALGGRIVHTGTVLHGKVSKIEHDNQAMF
CCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCEEEEECEEEECHHHHCCCCCCHHC
HGINNPMPVARYHSLMGDNLPEELEINARFDNIVMAIRHKQLPICGFQFHPESILTVEGT
CCCCCCCCHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCCCEECEEECCCEEEEECHH
KLLKQSVEWLLSTQH
HHHHHHHHHHHCCCC
>Mature Secondary Structure 
AHILFVDNFDSFTYNLVDQFRELGHQVTIFRNDYPLEDFLNKAQTTENCIVALSPGPGI
EEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCC
PAEAGNMLAIIKRLKGVVPMIGICLGHQAIIEALGGRIVHTGTVLHGKVSKIEHDNQAMF
CCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCEEEEECEEEECHHHHCCCCCCHHC
HGINNPMPVARYHSLMGDNLPEELEINARFDNIVMAIRHKQLPICGFQFHPESILTVEGT
CCCCCCCCHHHHHHHHCCCCCCCEEECCCHHHHHHHHHHCCCCEECEEECCCEEEEECHH
KLLKQSVEWLLSTQH
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]