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The map label for this gene is aroE

Identifier: 126208609

GI number: 126208609

Start: 1316031

End: 1316855

Strand: Reverse

Name: aroE

Synonym: APL_1139

Alternate gene names: 126208609

Gene position: 1316855-1316031 (Counterclockwise)

Preceding gene: 126208610

Following gene: 126208608

Centisome position: 57.9

GC content: 40.85

Gene sequence:

>825_bases
ATGAATCAGTATGCAGTATGGGGAAATCCGATTGCGCAAAGTAAGTCACCTCGTATTCATCAACTTTTTGGCGAGCAAAC
CGGTAAGTCTATTAGTTATGTTGCTAAGTTAGGGAATGAGCTGAATTTTGAGAACGAGCTTATGCAGTTTTTTGCAGAGG
GGGCAAAAGGGGCAAATATTACCGCACCGTTTAAAGAACGCGCGTTCTCACTTGCGGATGAATATAGTGAGAGCTGTTTG
TTAGCGGAAGCCTGTAATACATTAAAACGTTTAGATGACGGACGTTTATATGCGGATAATACTGATGGTTTTGGACTTTG
TTCTGATTTAGAACGTCTAGGTTGGCTGAAACCTCATCAGCGGGTACTTATTTTAGGCGCAGGCGGAGCGACTAAGGGGG
TACTTTTTCCGCTGCTAAAAGCGAAACAGCAGATTACTATCTATAATCGAACCTTAGAGAAAGCGGTTCGTTTAGCAGAA
AAATTTGCAAAATACGGCAAAATTCGGACCGCTTCATTGGAACAAATTGCAGAGCAACAATTTGATTTGATTATTAATGC
GACATCTTTAGGCTTACAAGGTAAATATGTGCCTGTTGCGGCTCATCTTTTAAAAAGTGCTGCGGTTTATGATATGCAAT
ATGCACCTGATATGCAGACACCGTTTTTAAATTATGCGCGTGAGTGCGGTGCAGTGCGTTATCAAGACGGTTTGGGCATG
TTGGTCGGTCAGGCAGGCTTTGCATTTAAACTTTGGGAAAATGAATTTCCGAACGTTGAAAAAGTGCTTAAACAATTGAA
AAATGAAATGGAAAATGCAAAATAA

Upstream 100 bases:

>100_bases
GGATTTTCCTGTATTGGAAGCGGAGACTGCTGGCAAAACCAATCCTCCTGAAATTCGAGATATTTTTACACAACATATTT
TTAGACAAGGATAAATAAAC

Downstream 100 bases:

>100_bases
GATTTCATAACCTATTATAAAATAAGCAAAATTTAGTTATTTTTATGACAAAATGAGCAATGTATAGCCAAATAATCAAA
TTTCTGACTTTTTTTTAAAA

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MNQYAVWGNPIAQSKSPRIHQLFGEQTGKSISYVAKLGNELNFENELMQFFAEGAKGANITAPFKERAFSLADEYSESCL
LAEACNTLKRLDDGRLYADNTDGFGLCSDLERLGWLKPHQRVLILGAGGATKGVLFPLLKAKQQITIYNRTLEKAVRLAE
KFAKYGKIRTASLEQIAEQQFDLIINATSLGLQGKYVPVAAHLLKSAAVYDMQYAPDMQTPFLNYARECGAVRYQDGLGM
LVGQAGFAFKLWENEFPNVEKVLKQLKNEMENAK

Sequences:

>Translated_274_residues
MNQYAVWGNPIAQSKSPRIHQLFGEQTGKSISYVAKLGNELNFENELMQFFAEGAKGANITAPFKERAFSLADEYSESCL
LAEACNTLKRLDDGRLYADNTDGFGLCSDLERLGWLKPHQRVLILGAGGATKGVLFPLLKAKQQITIYNRTLEKAVRLAE
KFAKYGKIRTASLEQIAEQQFDLIINATSLGLQGKYVPVAAHLLKSAAVYDMQYAPDMQTPFLNYARECGAVRYQDGLGM
LVGQAGFAFKLWENEFPNVEKVLKQLKNEMENAK
>Mature_274_residues
MNQYAVWGNPIAQSKSPRIHQLFGEQTGKSISYVAKLGNELNFENELMQFFAEGAKGANITAPFKERAFSLADEYSESCL
LAEACNTLKRLDDGRLYADNTDGFGLCSDLERLGWLKPHQRVLILGAGGATKGVLFPLLKAKQQITIYNRTLEKAVRLAE
KFAKYGKIRTASLEQIAEQQFDLIINATSLGLQGKYVPVAAHLLKSAAVYDMQYAPDMQTPFLNYARECGAVRYQDGLGM
LVGQAGFAFKLWENEFPNVEKVLKQLKNEMENAK

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=271, Percent_Identity=50.1845018450184, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1787983, Length=265, Percent_Identity=26.7924528301887, Blast_Score=73, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320332, Length=274, Percent_Identity=27.3722627737226, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_ACTP2 (A3N1E3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053834.1
- ProteinModelPortal:   A3N1E3
- SMR:   A3N1E3
- STRING:   A3N1E3
- GeneID:   4849556
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_1139
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   DQYVVFG
- PhylomeDB:   A3N1E3
- ProtClustDB:   PRK00258
- BioCyc:   APLE416269:APL_1139-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 30546; Mature: 30546

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQYAVWGNPIAQSKSPRIHQLFGEQTGKSISYVAKLGNELNFENELMQFFAEGAKGANI
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
TAPFKERAFSLADEYSESCLLAEACNTLKRLDDGRLYADNTDGFGLCSDLERLGWLKPHQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCCCCCCC
RVLILGAGGATKGVLFPLLKAKQQITIYNRTLEKAVRLAEKFAKYGKIRTASLEQIAEQQ
EEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FDLIINATSLGLQGKYVPVAAHLLKSAAVYDMQYAPDMQTPFLNYARECGAVRYQDGLGM
HHEEEEEHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEECCCCEE
LVGQAGFAFKLWENEFPNVEKVLKQLKNEMENAK
EECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQYAVWGNPIAQSKSPRIHQLFGEQTGKSISYVAKLGNELNFENELMQFFAEGAKGANI
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCC
TAPFKERAFSLADEYSESCLLAEACNTLKRLDDGRLYADNTDGFGLCSDLERLGWLKPHQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHCCCCCCC
RVLILGAGGATKGVLFPLLKAKQQITIYNRTLEKAVRLAEKFAKYGKIRTASLEQIAEQQ
EEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
FDLIINATSLGLQGKYVPVAAHLLKSAAVYDMQYAPDMQTPFLNYARECGAVRYQDGLGM
HHEEEEEHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEECCCCEE
LVGQAGFAFKLWENEFPNVEKVLKQLKNEMENAK
EECCCCCEEEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA