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The map label for this gene is dacB [H]

Identifier: 126208421

GI number: 126208421

Start: 1085816

End: 1087258

Strand: Direct

Name: dacB [H]

Synonym: APL_0945

Alternate gene names: 126208421

Gene position: 1085816-1087258 (Clockwise)

Preceding gene: 126208419

Following gene: 126208424

Centisome position: 47.74

GC content: 41.51

Gene sequence:

>1443_bases
ATGTTGTTAAAATCCGTTCGCTCATACATTCAAAATCTTTTACTGACGAGTATCTTTATTCCGAGTATTTCTTTTGCCGA
AATAAATACCTCGGAGCTTCTCACCAATTTACCGGCAGGTGCCTCCGCCAGTTTTATTGCGAAAAACCTCGAAACCAATC
AAATTATTACACAATATCAAAGTGATGTTTTTATGTTGCCGGCAAGTACACAAAAAGTATTTACCGCACTTGCCGCCAAA
TTGGTTTTATCGAATGATTTTCGCTTTCAAACGGCTTTATTAACGAACGGGAAAGTGGAAAACGGAATAATAAAAGGTGA
TTTAATCGCCAAATTCACAGGTGATCCGGATCTCACTAGCGGTCAGCTTTATCAGCTTATCGGGCAATTAAAAAAACAAG
GTATTGAAAAAATAGAAGGCAATCTCATTTTAGATACCTCCGTTTTTGCCAGTCATGATAAAGCGGCGGGTTGGATTTGG
AATGATTTAACTATGTGCTTTAACGCTCCGCCTGCCGCCGTCAATGTGGATAACAACTGCTTTTATGTCAATTTAGACAC
CAATCAAAAAGTCGGTGATTTTGCAAAAGTTAATGTTCCCGCCGCCTATCCGGTACAAGTATTTAGCTCGGCTTATATCG
TCGATAAAGCGGAAGCTCCTTATTGCCAGTTGGATGTCGTAGTTAATGACAACAACCGCTATCAAATCAAAGGCTGTATG
GCTCGCCAAGCGGAACCGTTCGGATTGAGTTTTTCCGTACAAGATCCCGCTACTTACGGTGCAAATTTGATTAAAGCGAA
TTTAAAGCGCCTAGGCATTGCTTTTGAAGGGAAAATTCAAGAACCGCTTAATGCGCAAAAAGGTACTTTGTTGGCGGAAC
ATTGGTCTGCTCCGTTACCGGATCTTTTAAAGAAAATGATGAAAAAATCCGATAACCAAATTGCGGATAGTCTATTCAGA
ACCATTGCCAATCAGCAGCATAATCGTCCGGCGTCTTTTCCGTTAGCTAGTCACGTATTACGCACTTTATTAAAATCTAA
AGCGGGCGTTACCTTTGGAAATAGTATTGTCGCAGACGGTTCAGGTCTTTCTCGTCATAACCAAATCAATGCGGAAACGA
TGTTGCAAGCCCTTGAATATATTGCAAAAAATGAAGCGCAATTACAGCTGTTTGAAACCTTCCCGATTGCGGGTGTGGAC
GGTACGATTTCCGGTAGGGGCTCTATCTCCGTCGAACCGCTGGCGAAAAATCTGATTGCCAAAACCGGTTCACTGAAAGG
GGTATATAATCTGGCGGGCTTTATGAAAAATGCGCGAGGTGAACGTATCGCCTTTGTACAATTTGTAAACGGCTATTCAA
CCGGCGAGCTGGAAAGTAAAACCAAACGTGCGCCGTTAGTGAATTTTGAAAATAAATTTTATATGGCGCTCTTTAACGAA
TAA

Upstream 100 bases:

>100_bases
ATAGGAATTTGTTTCATTATTTATCCTTTTCAAGTTTACTAAAATAGTTTAAAGGACTATTATTCAACGTCTTGTTAAAA
ATATCAACTCCCGAATTTAT

Downstream 100 bases:

>100_bases
TTTCCCTAAAAGGCACTCCTGACGAGTGCCTTTTACTTATGCAATTAAATCCTGATGACTTCTCCGCATTTTTACTTCAA
TATAACTACAACTCGGCTTG

Product: D-alanyl-D-alanine carboxypeptidase/endopeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; D-alanyl-D-alanine endopeptidase; DD-endopeptidase; Penicillin-binding protein 4; PBP-4 [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLLKSVRSYIQNLLLTSIFIPSISFAEINTSELLTNLPAGASASFIAKNLETNQIITQYQSDVFMLPASTQKVFTALAAK
LVLSNDFRFQTALLTNGKVENGIIKGDLIAKFTGDPDLTSGQLYQLIGQLKKQGIEKIEGNLILDTSVFASHDKAAGWIW
NDLTMCFNAPPAAVNVDNNCFYVNLDTNQKVGDFAKVNVPAAYPVQVFSSAYIVDKAEAPYCQLDVVVNDNNRYQIKGCM
ARQAEPFGLSFSVQDPATYGANLIKANLKRLGIAFEGKIQEPLNAQKGTLLAEHWSAPLPDLLKKMMKKSDNQIADSLFR
TIANQQHNRPASFPLASHVLRTLLKSKAGVTFGNSIVADGSGLSRHNQINAETMLQALEYIAKNEAQLQLFETFPIAGVD
GTISGRGSISVEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFVNGYSTGELESKTKRAPLVNFENKFYMALFNE

Sequences:

>Translated_480_residues
MLLKSVRSYIQNLLLTSIFIPSISFAEINTSELLTNLPAGASASFIAKNLETNQIITQYQSDVFMLPASTQKVFTALAAK
LVLSNDFRFQTALLTNGKVENGIIKGDLIAKFTGDPDLTSGQLYQLIGQLKKQGIEKIEGNLILDTSVFASHDKAAGWIW
NDLTMCFNAPPAAVNVDNNCFYVNLDTNQKVGDFAKVNVPAAYPVQVFSSAYIVDKAEAPYCQLDVVVNDNNRYQIKGCM
ARQAEPFGLSFSVQDPATYGANLIKANLKRLGIAFEGKIQEPLNAQKGTLLAEHWSAPLPDLLKKMMKKSDNQIADSLFR
TIANQQHNRPASFPLASHVLRTLLKSKAGVTFGNSIVADGSGLSRHNQINAETMLQALEYIAKNEAQLQLFETFPIAGVD
GTISGRGSISVEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFVNGYSTGELESKTKRAPLVNFENKFYMALFNE
>Mature_480_residues
MLLKSVRSYIQNLLLTSIFIPSISFAEINTSELLTNLPAGASASFIAKNLETNQIITQYQSDVFMLPASTQKVFTALAAK
LVLSNDFRFQTALLTNGKVENGIIKGDLIAKFTGDPDLTSGQLYQLIGQLKKQGIEKIEGNLILDTSVFASHDKAAGWIW
NDLTMCFNAPPAAVNVDNNCFYVNLDTNQKVGDFAKVNVPAAYPVQVFSSAYIVDKAEAPYCQLDVVVNDNNRYQIKGCM
ARQAEPFGLSFSVQDPATYGANLIKANLKRLGIAFEGKIQEPLNAQKGTLLAEHWSAPLPDLLKKMMKKSDNQIADSLFR
TIANQQHNRPASFPLASHVLRTLLKSKAGVTFGNSIVADGSGLSRHNQINAETMLQALEYIAKNEAQLQLFETFPIAGVD
GTISGRGSISVEPLAKNLIAKTGSLKGVYNLAGFMKNARGERIAFVQFVNGYSTGELESKTKRAPLVNFENKFYMALFNE

Specific function: Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction [H]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S13 family [H]

Homologues:

Organism=Escherichia coli, GI1789573, Length=468, Percent_Identity=46.3675213675214, Blast_Score=436, Evalue=1e-123,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR000667 [H]

Pfam domain/function: PF02113 Peptidase_S13 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 52515; Mature: 52515

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKSVRSYIQNLLLTSIFIPSISFAEINTSELLTNLPAGASASFIAKNLETNQIITQYQ
CCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHC
SDVFMLPASTQKVFTALAAKLVLSNDFRFQTALLTNGKVENGIIKGDLIAKFTGDPDLTS
CCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCEEEEECCCCCCCC
GQLYQLIGQLKKQGIEKIEGNLILDTSVFASHDKAAGWIWNDLTMCFNAPPAAVNVDNNC
HHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCEEECCHHHEECCCCCEEEECCCE
FYVNLDTNQKVGDFAKVNVPAAYPVQVFSSAYIVDKAEAPYCQLDVVVNDNNRYQIKGCM
EEEEECCCCCCCCEEEECCCCCCHHHHHCCEEEEECCCCCEEEEEEEECCCCEEEEEEEH
ARQAEPFGLSFSVQDPATYGANLIKANLKRLGIAFEGKIQEPLNAQKGTLLAEHWSAPLP
HCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCEEHHHCCCCHH
DLLKKMMKKSDNQIADSLFRTIANQQHNRPASFPLASHVLRTLLKSKAGVTFGNSIVADG
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEECCEEEECC
SGLSRHNQINAETMLQALEYIAKNEAQLQLFETFPIAGVDGTISGRGSISVEPLAKNLIA
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHH
KTGSLKGVYNLAGFMKNARGERIAFVQFVNGYSTGELESKTKRAPLVNFENKFYMALFNE
HCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHCCCCEEEECCEEEEEEECC
>Mature Secondary Structure
MLLKSVRSYIQNLLLTSIFIPSISFAEINTSELLTNLPAGASASFIAKNLETNQIITQYQ
CCHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHC
SDVFMLPASTQKVFTALAAKLVLSNDFRFQTALLTNGKVENGIIKGDLIAKFTGDPDLTS
CCEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCEEECCEEEEECCCCCCCC
GQLYQLIGQLKKQGIEKIEGNLILDTSVFASHDKAAGWIWNDLTMCFNAPPAAVNVDNNC
HHHHHHHHHHHHHHHHHHCCCEEEEEHHHCCCCCCCCEEECCHHHEECCCCCEEEECCCE
FYVNLDTNQKVGDFAKVNVPAAYPVQVFSSAYIVDKAEAPYCQLDVVVNDNNRYQIKGCM
EEEEECCCCCCCCEEEECCCCCCHHHHHCCEEEEECCCCCEEEEEEEECCCCEEEEEEEH
ARQAEPFGLSFSVQDPATYGANLIKANLKRLGIAFEGKIQEPLNAQKGTLLAEHWSAPLP
HCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCEEEECCCCCCCCCCCCCEEHHHCCCCHH
DLLKKMMKKSDNQIADSLFRTIANQQHNRPASFPLASHVLRTLLKSKAGVTFGNSIVADG
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCEECCEEEECC
SGLSRHNQINAETMLQALEYIAKNEAQLQLFETFPIAGVDGTISGRGSISVEPLAKNLIA
CCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEHHHHHHHHHH
KTGSLKGVYNLAGFMKNARGERIAFVQFVNGYSTGELESKTKRAPLVNFENKFYMALFNE
HCCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHCCCCEEEECCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]