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The map label for this gene is ogt [H]

Identifier: 126208410

GI number: 126208410

Start: 1075530

End: 1076093

Strand: Direct

Name: ogt [H]

Synonym: APL_0934

Alternate gene names: 126208410

Gene position: 1075530-1076093 (Clockwise)

Preceding gene: 126208409

Following gene: 126208419

Centisome position: 47.29

GC content: 43.44

Gene sequence:

>564_bases
ATGAAATCAACGATTTACTATCAATATTACGATTCACTGGTCGGCAAGCTGTTATTGATTGCCAATGATAAAGGTTTAAT
CGGCATTGAGTTTGAGCAAGAACAGCTCACAAGCGATGCGCAAAAATGGCAAGAAGCTAACGAAACAAGCGGTCAAATTT
TCGAAATTTTTTGCAAAACGCGCGATATTTTAGACCGCTATTTTGCCGGTGAGAAATTGGAGTTTAGACATTTGGATTTT
CTGGCACCGCAAGGTACCGAATTTCAGAAATCCGTCTGGCAGATTTTATTAACCATTCCTTACGGCAAAACCACCAGTTA
CGGTGAAATCGCACGACAACTCGGTAAACCGAATGCAATGCGAGCGGTAGGCGGTGCGGTCGGGCGCAATCCGATTTCAA
TTATCGTGCCTTGCCACCGCGTATTAGGCAAGAGCCAAACTTTGACCGGCTTTGGCGGCGGCTTACCGGCTAAACGCTAT
CTATTGGCACTCGAAGGAATTCATTTTAAAGATAAAGGGATTGAATTCGTCAATCCGAAACATAAAAAATGGGATAAAGC
CTAA

Upstream 100 bases:

>100_bases
AACTACAAATTTTAATGCCTAACTCAAAATAGGAAATTACGAAGATGCCAAAGGCGTGTAAACTGATTACACGCCTTTTT
ATTTACTTTAAGGAGAAATA

Downstream 100 bases:

>100_bases
AGACGTTATCCCCTTTCGATTAGTAATTTTCTTTTACCCAATTTTCCGCTAATGCTAAATCCGCTTGCCACTGTTGAGCG
ATTTGGAACACCCATTGTGC

Product: methylated-DNA--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MKSTIYYQYYDSLVGKLLLIANDKGLIGIEFEQEQLTSDAQKWQEANETSGQIFEIFCKTRDILDRYFAGEKLEFRHLDF
LAPQGTEFQKSVWQILLTIPYGKTTSYGEIARQLGKPNAMRAVGGAVGRNPISIIVPCHRVLGKSQTLTGFGGGLPAKRY
LLALEGIHFKDKGIEFVNPKHKKWDKA

Sequences:

>Translated_187_residues
MKSTIYYQYYDSLVGKLLLIANDKGLIGIEFEQEQLTSDAQKWQEANETSGQIFEIFCKTRDILDRYFAGEKLEFRHLDF
LAPQGTEFQKSVWQILLTIPYGKTTSYGEIARQLGKPNAMRAVGGAVGRNPISIIVPCHRVLGKSQTLTGFGGGLPAKRY
LLALEGIHFKDKGIEFVNPKHKKWDKA
>Mature_187_residues
MKSTIYYQYYDSLVGKLLLIANDKGLIGIEFEQEQLTSDAQKWQEANETSGQIFEIFCKTRDILDRYFAGEKLEFRHLDF
LAPQGTEFQKSVWQILLTIPYGKTTSYGEIARQLGKPNAMRAVGGAVGRNPISIIVPCHRVLGKSQTLTGFGGGLPAKRY
LLALEGIHFKDKGIEFVNPKHKKWDKA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=162, Percent_Identity=33.9506172839506, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1787596, Length=149, Percent_Identity=42.9530201342282, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1788542, Length=167, Percent_Identity=32.3353293413174, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI1786659, Length=86, Percent_Identity=38.3720930232558, Blast_Score=65, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI115533068, Length=85, Percent_Identity=44.7058823529412, Blast_Score=83, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=85, Percent_Identity=44.7058823529412, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=44.4444444444444, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21116; Mature: 21116

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTIYYQYYDSLVGKLLLIANDKGLIGIEFEQEQLTSDAQKWQEANETSGQIFEIFCKT
CCCEEEHHHHHHHHHEEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RDILDRYFAGEKLEFRHLDFLAPQGTEFQKSVWQILLTIPYGKTTSYGEIARQLGKPNAM
HHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCHHH
RAVGGAVGRNPISIIVPCHRVLGKSQTLTGFGGGLPAKRYLLALEGIHFKDKGIEFVNPK
HHHHHHCCCCCEEEEEECHHHHCCCCEECCCCCCCCHHHHHHHHCCCEECCCCCCCCCCC
HKKWDKA
CCCCCCC
>Mature Secondary Structure
MKSTIYYQYYDSLVGKLLLIANDKGLIGIEFEQEQLTSDAQKWQEANETSGQIFEIFCKT
CCCEEEHHHHHHHHHEEEEEECCCCEEEEEECHHHHHHHHHHHHHCCCCCCHHHHHHHHH
RDILDRYFAGEKLEFRHLDFLAPQGTEFQKSVWQILLTIPYGKTTSYGEIARQLGKPNAM
HHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHEEEECCCCCCCCHHHHHHHHCCCHHH
RAVGGAVGRNPISIIVPCHRVLGKSQTLTGFGGGLPAKRYLLALEGIHFKDKGIEFVNPK
HHHHHHCCCCCEEEEEECHHHHCCCCEECCCCCCCCHHHHHHHHCCCEECCCCCCCCCCC
HKKWDKA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]