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The map label for this gene is gsiB [H]

Identifier: 126208324

GI number: 126208324

Start: 983413

End: 984987

Strand: Direct

Name: gsiB [H]

Synonym: APL_0848

Alternate gene names: 126208324

Gene position: 983413-984987 (Clockwise)

Preceding gene: 126208319

Following gene: 126208325

Centisome position: 43.24

GC content: 43.87

Gene sequence:

>1575_bases
ATGACTTCTCATTTTTCTCATAACGACTCACGTCGCCACTTCATGAAGCTTCTTGCCGGTGTCGGAGCAGGCTTTGCATT
CTCCGGTACCTTAGGTCCTTTCTCTAATAATGCATTTGCCGCAGCAGGTAAAAGTATTGAAGCAGGGATTGCTTATCCGA
TCTCAACCGGTTTTGACCCGCTTACTTCAAGCGGTGCATCTTCTATGGCGGCGAACTTACATATTTTTGAAGGTTTAGTG
GATTTACACCCGGCAACTCGCCAACCTTATTTAGCTTTAGCGGCAAAAGAGCCGGAACAAAAAGATGAGGTGACATACCA
TATTACCTTACGTGAAGGGGCAACCTTCCACGATGGTAAACCGGTCACTACCGAAGATGTGGTTTACTCGTTTGAACGTG
TGTTAGATCCGGCTAAAGCTTCACTGTTCGCGCAATTTATTCCGTTTATCGCTTCGGTAAAAGCACTTGACAATAAAGTG
GTCGAATTCAAATTAAAATATCCGTTCGCTTTATTTAAAGAACGTTTAACCATCGTCAAAATCGTGCCGAAACATATCGT
AGAAGCCGGTCAATCCGCCTTTGATGCCAAACCTGTCGGTTCAGGTCCTTATAAATTTGTTTCCGCAACCAAAGATGACC
GTATTGTCTTTGAAGCCAATACCTCTTATAACGGTATGTATCCGGCTAAAGTAGATAAAATGACGTGGTTCTTATTATCA
GATGATGCCGCTCGTGTAACCGCACAAGAATCCGGACGTGTACAAGCGATTGAATCCGTACCGTACCTTGATGCGGAACG
CTTAAAACGTAAAGGAAAAGTGGAATCAGTACAATCTTTCGGCTTACTATTCTTAATGTTTAACTGTGAAAAAGCACCGT
TTGATAACCCGAAAGTACGCCAGGCGTTACATTACGGTTTAGATACGCAAAAATTAATCGACATTGTATTCTTAGGCAAT
GCGAAAGCGGCAAGCTCTTACGTACAAGATACCCATCCTGATTATGTAAAAGCCGCCAGCCAATATGATTTTGATAAAGC
GAAAGCGGAAAGCCTATTAGCGGAAGCGGGTATCAAAGAATTAAAATTTGAATTACTTGCAACCGATCACGCTTGGGTAA
AAGAATGTGCGCCGCTTATTCTTGAATCTTGGAATGCGTTAAAAGGTGTGAAAGTAACGCTTCAACATTTACAATCCGGT
GCGTTATACGGCACGCACGTTGATAAAGGTGCGTTTGAAGTGGTTATCGCACCGGGCGATCCGTCCGTATTCGGTAACGA
CTTAGACTTATTATTAAGCTGGTGGTACCGCGGTGACGTATGGCCGAAACGTCGTTTCCGTTGGGCAAATACGCCTGAAT
ATGCCGAAGTACAAAAATTACTGGATGAAGCGGCGAAAAATCCGGCGGGTGCAAAAGAAGCATGGACCAAAGCAATCAAT
ATTATTGCCGAACAAGTGCCGCTTTACCCGATCGTACATCGTAAATTACCGACCGCATGGAGCGATAAATCACTTACCGA
TTTCCAACCGTTACCGACAACAGGCTTGTCATTCTTAGGCGTCGGTCGTAAATAA

Upstream 100 bases:

>100_bases
TTTATCACTTCATTTTTAAATCTAAAAAAGAACACTATCATTGTTCAGATTAAGGTTGTGCTGAATATATCTAATCTAAT
TAAACATAAAGAGGTGTGCT

Downstream 100 bases:

>100_bases
CCTCTAATAAAATGCCCTTTGTCGTCCAAAGGCAAAGGGTATTACTTAATATAACTCTCTTAATTAAATCTTTACCCTTC
AAAAATGAGGGAGGCTCAAT

Product: putative ABC transporter periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MTSHFSHNDSRRHFMKLLAGVGAGFAFSGTLGPFSNNAFAAAGKSIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLV
DLHPATRQPYLALAAKEPEQKDEVTYHITLREGATFHDGKPVTTEDVVYSFERVLDPAKASLFAQFIPFIASVKALDNKV
VEFKLKYPFALFKERLTIVKIVPKHIVEAGQSAFDAKPVGSGPYKFVSATKDDRIVFEANTSYNGMYPAKVDKMTWFLLS
DDAARVTAQESGRVQAIESVPYLDAERLKRKGKVESVQSFGLLFLMFNCEKAPFDNPKVRQALHYGLDTQKLIDIVFLGN
AKAASSYVQDTHPDYVKAASQYDFDKAKAESLLAEAGIKELKFELLATDHAWVKECAPLILESWNALKGVKVTLQHLQSG
ALYGTHVDKGAFEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRWANTPEYAEVQKLLDEAAKNPAGAKEAWTKAIN
IIAEQVPLYPIVHRKLPTAWSDKSLTDFQPLPTTGLSFLGVGRK

Sequences:

>Translated_524_residues
MTSHFSHNDSRRHFMKLLAGVGAGFAFSGTLGPFSNNAFAAAGKSIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLV
DLHPATRQPYLALAAKEPEQKDEVTYHITLREGATFHDGKPVTTEDVVYSFERVLDPAKASLFAQFIPFIASVKALDNKV
VEFKLKYPFALFKERLTIVKIVPKHIVEAGQSAFDAKPVGSGPYKFVSATKDDRIVFEANTSYNGMYPAKVDKMTWFLLS
DDAARVTAQESGRVQAIESVPYLDAERLKRKGKVESVQSFGLLFLMFNCEKAPFDNPKVRQALHYGLDTQKLIDIVFLGN
AKAASSYVQDTHPDYVKAASQYDFDKAKAESLLAEAGIKELKFELLATDHAWVKECAPLILESWNALKGVKVTLQHLQSG
ALYGTHVDKGAFEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRWANTPEYAEVQKLLDEAAKNPAGAKEAWTKAIN
IIAEQVPLYPIVHRKLPTAWSDKSLTDFQPLPTTGLSFLGVGRK
>Mature_523_residues
TSHFSHNDSRRHFMKLLAGVGAGFAFSGTLGPFSNNAFAAAGKSIEAGIAYPISTGFDPLTSSGASSMAANLHIFEGLVD
LHPATRQPYLALAAKEPEQKDEVTYHITLREGATFHDGKPVTTEDVVYSFERVLDPAKASLFAQFIPFIASVKALDNKVV
EFKLKYPFALFKERLTIVKIVPKHIVEAGQSAFDAKPVGSGPYKFVSATKDDRIVFEANTSYNGMYPAKVDKMTWFLLSD
DAARVTAQESGRVQAIESVPYLDAERLKRKGKVESVQSFGLLFLMFNCEKAPFDNPKVRQALHYGLDTQKLIDIVFLGNA
KAASSYVQDTHPDYVKAASQYDFDKAKAESLLAEAGIKELKFELLATDHAWVKECAPLILESWNALKGVKVTLQHLQSGA
LYGTHVDKGAFEVVIAPGDPSVFGNDLDLLLSWWYRGDVWPKRRFRWANTPEYAEVQKLLDEAAKNPAGAKEAWTKAINI
IAEQVPLYPIVHRKLPTAWSDKSLTDFQPLPTTGLSFLGVGRK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=495, Percent_Identity=28.2828282828283, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789966, Length=331, Percent_Identity=28.3987915407855, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI87081878, Length=473, Percent_Identity=26.8498942917548, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1787762, Length=385, Percent_Identity=25.974025974026, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787495, Length=414, Percent_Identity=25.6038647342995, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787551, Length=498, Percent_Identity=23.8955823293173, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789887, Length=413, Percent_Identity=27.3607748184019, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789397, Length=484, Percent_Identity=24.1735537190083, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57856; Mature: 57725

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSHFSHNDSRRHFMKLLAGVGAGFAFSGTLGPFSNNAFAAAGKSIEAGIAYPISTGFDP
CCCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHCCCCCCCCEEEECCCCCCC
LTSSGASSMAANLHIFEGLVDLHPATRQPYLALAAKEPEQKDEVTYHITLREGATFHDGK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCEECCCC
PVTTEDVVYSFERVLDPAKASLFAQFIPFIASVKALDNKVVEFKLKYPFALFKERLTIVK
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
IVPKHIVEAGQSAFDAKPVGSGPYKFVSATKDDRIVFEANTSYNGMYPAKVDKMTWFLLS
HHHHHHHHCCCHHCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEEE
DDAARVTAQESGRVQAIESVPYLDAERLKRKGKVESVQSFGLLFLMFNCEKAPFDNPKVR
CCCCEEECCCCCCEEEECCCCCCCHHHHHHCCCHHHHHHCCEEEEEEECCCCCCCCHHHH
QALHYGLDTQKLIDIVFLGNAKAASSYVQDTHPDYVKAASQYDFDKAKAESLLAEAGIKE
HHHHHCCCHHHEEEEEEECCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCHHH
LKFELLATDHAWVKECAPLILESWNALKGVKVTLQHLQSGALYGTHVDKGAFEVVIAPGD
HHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCC
PSVFGNDLDLLLSWWYRGDVWPKRRFRWANTPEYAEVQKLLDEAAKNPAGAKEAWTKAIN
CCCCCCCHHHHHHHHHCCCCCHHHCEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
IIAEQVPLYPIVHRKLPTAWSDKSLTDFQPLPTTGLSFLGVGRK
HHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
>Mature Secondary Structure 
TSHFSHNDSRRHFMKLLAGVGAGFAFSGTLGPFSNNAFAAAGKSIEAGIAYPISTGFDP
CCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHCCCCCCCCEEEECCCCCCC
LTSSGASSMAANLHIFEGLVDLHPATRQPYLALAAKEPEQKDEVTYHITLREGATFHDGK
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEEECCCEECCCC
PVTTEDVVYSFERVLDPAKASLFAQFIPFIASVKALDNKVVEFKLKYPFALFKERLTIVK
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHH
IVPKHIVEAGQSAFDAKPVGSGPYKFVSATKDDRIVFEANTSYNGMYPAKVDKMTWFLLS
HHHHHHHHCCCHHCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEEEE
DDAARVTAQESGRVQAIESVPYLDAERLKRKGKVESVQSFGLLFLMFNCEKAPFDNPKVR
CCCCEEECCCCCCEEEECCCCCCCHHHHHHCCCHHHHHHCCEEEEEEECCCCCCCCHHHH
QALHYGLDTQKLIDIVFLGNAKAASSYVQDTHPDYVKAASQYDFDKAKAESLLAEAGIKE
HHHHHCCCHHHEEEEEEECCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHCHHH
LKFELLATDHAWVKECAPLILESWNALKGVKVTLQHLQSGALYGTHVDKGAFEVVIAPGD
HHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCEEEEEECCC
PSVFGNDLDLLLSWWYRGDVWPKRRFRWANTPEYAEVQKLLDEAAKNPAGAKEAWTKAIN
CCCCCCCHHHHHHHHHCCCCCHHHCEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
IIAEQVPLYPIVHRKLPTAWSDKSLTDFQPLPTTGLSFLGVGRK
HHHHHCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA