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The map label for this gene is ispD

Identifier: 126208278

GI number: 126208278

Start: 924298

End: 924984

Strand: Direct

Name: ispD

Synonym: APL_0802

Alternate gene names: 126208278

Gene position: 924298-924984 (Clockwise)

Preceding gene: 126208277

Following gene: 126208279

Centisome position: 40.64

GC content: 41.48

Gene sequence:

>687_bases
ATGACACGAAAAATTATTGCGGTAATTCCTGCCTCGGGTGTCGGTAGCCGTATGCAAGCCGGTTTACCCAAACAATATTT
AAAATTACAAAATAAAACGATTTTAGAGCATACGCTCGAAATTTTTTTAGCACATCCGGATATTGAAAAAATTGTTGTTG
CCGTTGCCGAAACCGATCCGTTTTATCCTCAAGTCGCTTTATTAGACTCGCCTAAAATCCAAATCGTATTTGGCGGAGAA
ACTCGGGCGCATTCCGTTTTTAATGCGCTGCAAGTTATTGAAGATGATAGTTGGGTATTAGTACATGATGCGGCACGTCC
CTGTTTAAAGCGGTCGGATTTAGATAAACTTTTACAAATCGATGATAAACAGGGTGCGATATTAGCGACTCCGGCAATCG
ATACGATGAAAAGAGCCGACGGTAACAAAATTATGCGCACTGAAGATCGTTCAACGCTTTGGCATGCACTTACCCCTCAA
TTTTTTCCTACTCGGCTACTGAAGCAAGCGCTTATTTCAGCATTTAAGAAAAATTTAACCGTAACGGACGAGGCTTCCGC
AATGGAATTTAGCGGTTATCAGCCACGTCTTATTGCGGGGCGCAGCGATAACTTAAAAATTACTCGCCCTGAAGATTTAG
CCTTAGCGGAATTTTACTTAACCCAAAACACGGAGAAAAAAATATGA

Upstream 100 bases:

>100_bases
CGCACTCGAAGAACGGGCTCGCTTCGAACGCGAGATGGTGAAAAGTGACGAAACTTTTTATCGCATTGTACCTCGTAATC
AATAAATCATAGAAACATAA

Downstream 100 bases:

>100_bases
TTCGTATCGGACACGGTTTCGATGTACATGCGTTTGGACAGGACAGACCGTTAATGATTTGCGGTGTGGAAGTGCCTTAT
CATACCGGTTTTATCGCACA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MTRKIIAVIPASGVGSRMQAGLPKQYLKLQNKTILEHTLEIFLAHPDIEKIVVAVAETDPFYPQVALLDSPKIQIVFGGE
TRAHSVFNALQVIEDDSWVLVHDAARPCLKRSDLDKLLQIDDKQGAILATPAIDTMKRADGNKIMRTEDRSTLWHALTPQ
FFPTRLLKQALISAFKKNLTVTDEASAMEFSGYQPRLIAGRSDNLKITRPEDLALAEFYLTQNTEKKI

Sequences:

>Translated_228_residues
MTRKIIAVIPASGVGSRMQAGLPKQYLKLQNKTILEHTLEIFLAHPDIEKIVVAVAETDPFYPQVALLDSPKIQIVFGGE
TRAHSVFNALQVIEDDSWVLVHDAARPCLKRSDLDKLLQIDDKQGAILATPAIDTMKRADGNKIMRTEDRSTLWHALTPQ
FFPTRLLKQALISAFKKNLTVTDEASAMEFSGYQPRLIAGRSDNLKITRPEDLALAEFYLTQNTEKKI
>Mature_227_residues
TRKIIAVIPASGVGSRMQAGLPKQYLKLQNKTILEHTLEIFLAHPDIEKIVVAVAETDPFYPQVALLDSPKIQIVFGGET
RAHSVFNALQVIEDDSWVLVHDAARPCLKRSDLDKLLQIDDKQGAILATPAIDTMKRADGNKIMRTEDRSTLWHALTPQF
FPTRLLKQALISAFKKNLTVTDEASAMEFSGYQPRLIAGRSDNLKITRPEDLALAEFYLTQNTEKKI

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=240, Percent_Identity=26.25, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1789104, Length=227, Percent_Identity=51.5418502202643, Blast_Score=249, Evalue=7e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_ACTP2 (A3N0G2)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053503.1
- ProteinModelPortal:   A3N0G2
- SMR:   A3N0G2
- STRING:   A3N0G2
- GeneID:   4850225
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0802
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   GYGMPKA
- PhylomeDB:   A3N0G2
- ProtClustDB:   PRK00155
- BioCyc:   APLE416269:APL_0802-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25537; Mature: 25406

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKIIAVIPASGVGSRMQAGLPKQYLKLQNKTILEHTLEIFLAHPDIEKIVVAVAETDP
CCCEEEEEECCCCCCCHHHCCCCHHHHHHCCCHHHHHHHHHEEECCCHHHEEEEEECCCC
FYPQVALLDSPKIQIVFGGETRAHSVFNALQVIEDDSWVLVHDAARPCLKRSDLDKLLQI
CCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHHHHC
DDKQGAILATPAIDTMKRADGNKIMRTEDRSTLWHALTPQFFPTRLLKQALISAFKKNLT
CCCCCCEEECCCHHHHHHCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCE
VTDEASAMEFSGYQPRLIAGRSDNLKITRPEDLALAEFYLTQNTEKKI
ECCCCCCEECCCCCCEEEECCCCCEEEECCCCCEEEEEEEECCCCCCC
>Mature Secondary Structure 
TRKIIAVIPASGVGSRMQAGLPKQYLKLQNKTILEHTLEIFLAHPDIEKIVVAVAETDP
CCEEEEEECCCCCCCHHHCCCCHHHHHHCCCHHHHHHHHHEEECCCHHHEEEEEECCCC
FYPQVALLDSPKIQIVFGGETRAHSVFNALQVIEDDSWVLVHDAARPCLKRSDLDKLLQI
CCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCHHHHHHC
DDKQGAILATPAIDTMKRADGNKIMRTEDRSTLWHALTPQFFPTRLLKQALISAFKKNLT
CCCCCCEEECCCHHHHHHCCCCCEEEECCCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCE
VTDEASAMEFSGYQPRLIAGRSDNLKITRPEDLALAEFYLTQNTEKKI
ECCCCCCEECCCCCCEEEECCCCCEEEECCCCCEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA