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The map label for this gene is queA

Identifier: 126208243

GI number: 126208243

Start: 874210

End: 875295

Strand: Direct

Name: queA

Synonym: APL_0765

Alternate gene names: 126208243

Gene position: 874210-875295 (Clockwise)

Preceding gene: 126208242

Following gene: 126208246

Centisome position: 38.44

GC content: 43.74

Gene sequence:

>1086_bases
GTGTTAGTTTCTGATTTTCATTTTGATTTGCCTGATGAACTGATTGCCCGCTACCCTACAGAGGAACGAACGGCAAGCCG
CCTGTTACATTTAAACGGTGAAACGGGTCACTTTGAAGATAAACAATTTTTCGATTTACTTGACCAGATTAATGAAGGCG
ATTTATTAATTTTTAATAATACTCGCGTGATCCCTGCCCGTTTATACGGCAGAAAAGCCAGCGGTGGTAAATTAGAAATA
TTAGTGGAACGAGTATTAGACGAACACCGTTGTTTAGCACATGTTCGAGCCTCAAAAGCACCGAAAGAAGGTGCGGAACT
GGTACTTGGCGAAGATAAATTAGGCGAAGGTAACGGTTTTAAAGCGATCATGACTGCTCGTCATGACGCCTTGTTCGAAT
TACATTTTAATGAGGAACAACCCTTGTTTGATCTGCTCCAACAAGCCGGTCATATGCCGTTACCACCTTATATCGACCGT
CCGGACGAAGATGCCGATCAAGAACGCTATCAAACCGTTTACAGTAAAGTTTTGGGCGCGGTTGCGGCTCCGACCGCCGG
TTTACACTTTGATAATCCGACATTGGAAAAGCTGAAAGCTAAAGGCGTTAATATCGCGTTCGTTACATTGCATGTCGGTG
CAGGTACGTTCCAACCGGTACGCGTAGATAATATTCTGGATCATAAAATGCACGCGGAATATGCGGAAGTAAGCCAAGCA
GTCGTCGATCAAATTTTAGCGACTAAAGCCACAGGTAAACGTGTGATTGCCGTCGGCACGACCTCGGTTCGTTCGATTGA
AAGTGCGGCGCAAGCGGCAGAAAAAGAAGGAAAATTAATCACACCGTTTTTCTCCGACACCAGCATTTTCCTCTACCCGG
GCAAAACCTTTCGTATCGTTGATGCTTTAGTGACAAATTTCCATTTACCCGAATCAACGCTGATTATGTTAGTTTCCGCA
TTTGCCGGTTACCGTAATACCATGAAAGCCTATCAACATGCGGTAGAAGCGAAATACCGTTTCTTTAGTTATGGTGATGC
GATGTTTATCAATAAAAATCCTAATGCGTTAAATGATCTCCCATAA

Upstream 100 bases:

>100_bases
ACCGTAATAAGCTCAGTAAAGCAATTAATGATATAGTAGCATTGTTACATTAACCTACTGCCCCGTTTTGGGGCAGTTGA
TTTTTAGAGGATTATATTCC

Downstream 100 bases:

>100_bases
TCTCAATCGCCACGTTTTACAAACGTGGCTTTTTTTATCTATTTTTTCCGTATAAACGCCGATACCAAGGTGAAAAATCG
TTTCGAGCCGTCTTTAATTG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MLVSDFHFDLPDELIARYPTEERTASRLLHLNGETGHFEDKQFFDLLDQINEGDLLIFNNTRVIPARLYGRKASGGKLEI
LVERVLDEHRCLAHVRASKAPKEGAELVLGEDKLGEGNGFKAIMTARHDALFELHFNEEQPLFDLLQQAGHMPLPPYIDR
PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNPTLEKLKAKGVNIAFVTLHVGAGTFQPVRVDNILDHKMHAEYAEVSQA
VVDQILATKATGKRVIAVGTTSVRSIESAAQAAEKEGKLITPFFSDTSIFLYPGKTFRIVDALVTNFHLPESTLIMLVSA
FAGYRNTMKAYQHAVEAKYRFFSYGDAMFINKNPNALNDLP

Sequences:

>Translated_361_residues
MLVSDFHFDLPDELIARYPTEERTASRLLHLNGETGHFEDKQFFDLLDQINEGDLLIFNNTRVIPARLYGRKASGGKLEI
LVERVLDEHRCLAHVRASKAPKEGAELVLGEDKLGEGNGFKAIMTARHDALFELHFNEEQPLFDLLQQAGHMPLPPYIDR
PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNPTLEKLKAKGVNIAFVTLHVGAGTFQPVRVDNILDHKMHAEYAEVSQA
VVDQILATKATGKRVIAVGTTSVRSIESAAQAAEKEGKLITPFFSDTSIFLYPGKTFRIVDALVTNFHLPESTLIMLVSA
FAGYRNTMKAYQHAVEAKYRFFSYGDAMFINKNPNALNDLP
>Mature_361_residues
MLVSDFHFDLPDELIARYPTEERTASRLLHLNGETGHFEDKQFFDLLDQINEGDLLIFNNTRVIPARLYGRKASGGKLEI
LVERVLDEHRCLAHVRASKAPKEGAELVLGEDKLGEGNGFKAIMTARHDALFELHFNEEQPLFDLLQQAGHMPLPPYIDR
PDEDADQERYQTVYSKVLGAVAAPTAGLHFDNPTLEKLKAKGVNIAFVTLHVGAGTFQPVRVDNILDHKMHAEYAEVSQA
VVDQILATKATGKRVIAVGTTSVRSIESAAQAAEKEGKLITPFFSDTSIFLYPGKTFRIVDALVTNFHLPESTLIMLVSA
FAGYRNTMKAYQHAVEAKYRFFSYGDAMFINKNPNALNDLP

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=359, Percent_Identity=70.4735376044568, Blast_Score=514, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_ACTP2 (A3N0C7)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053468.1
- ProteinModelPortal:   A3N0C7
- SMR:   A3N0C7
- STRING:   A3N0C7
- GeneID:   4849804
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0765
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- PhylomeDB:   A3N0C7
- ProtClustDB:   PRK00147
- BioCyc:   APLE416269:APL_0765-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 40242; Mature: 40242

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVSDFHFDLPDELIARYPTEERTASRLLHLNGETGHFEDKQFFDLLDQINEGDLLIFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCEEEECC
TRVIPARLYGRKASGGKLEILVERVLDEHRCLAHVRASKAPKEGAELVLGEDKLGEGNGF
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCE
KAIMTARHDALFELHFNEEQPLFDLLQQAGHMPLPPYIDRPDEDADQERYQTVYSKVLGA
EEEEEECCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAAPTAGLHFDNPTLEKLKAKGVNIAFVTLHVGAGTFQPVRVDNILDHKMHAEYAEVSQA
HHCCCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHH
VVDQILATKATGKRVIAVGTTSVRSIESAAQAAEKEGKLITPFFSDTSIFLYPGKTFRIV
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHH
DALVTNFHLPESTLIMLVSAFAGYRNTMKAYQHAVEAKYRFFSYGDAMFINKNPNALNDL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCC
P
C
>Mature Secondary Structure
MLVSDFHFDLPDELIARYPTEERTASRLLHLNGETGHFEDKQFFDLLDQINEGDLLIFNN
CCCCCCCCCCCHHHHHHCCCHHHHHHHEEEECCCCCCCCHHHHHHHHHCCCCCCEEEECC
TRVIPARLYGRKASGGKLEILVERVLDEHRCLAHVRASKAPKEGAELVLGEDKLGEGNGF
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCE
KAIMTARHDALFELHFNEEQPLFDLLQQAGHMPLPPYIDRPDEDADQERYQTVYSKVLGA
EEEEEECCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
VAAPTAGLHFDNPTLEKLKAKGVNIAFVTLHVGAGTFQPVRVDNILDHKMHAEYAEVSQA
HHCCCCCCCCCCCHHHHHHHCCCEEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHH
VVDQILATKATGKRVIAVGTTSVRSIESAAQAAEKEGKLITPFFSDTSIFLYPGKTFRIV
HHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHH
DALVTNFHLPESTLIMLVSAFAGYRNTMKAYQHAVEAKYRFFSYGDAMFINKNPNALNDL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEECCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA