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The map label for this gene is dnaB [H]

Identifier: 126208193

GI number: 126208193

Start: 822613

End: 824076

Strand: Reverse

Name: dnaB [H]

Synonym: APL_0713

Alternate gene names: 126208193

Gene position: 824076-822613 (Counterclockwise)

Preceding gene: 126208201

Following gene: 126208192

Centisome position: 36.23

GC content: 43.31

Gene sequence:

>1464_bases
ATGCCTGAATACACGCCAACTAATAAAGAGAATTCAAGGGACAAGCAAGTGGAACAAATTGCGATTGCTCCTCACTCTAT
CGAAGCGGAACAAGCCGTATTAGGCGGTATTATGCTCAATAACGAACATTGGGATAACGTATCCGAACGTATTCAAGCCG
GTGATTTTTATAACTATGCACATCGTACTATTTTTGAGCAAATGGTGGAGTTGGTACGCCATAATCAACCGATTGATATT
ATTACGTTAGATCAAGCGTTGAAAGATAAAGGCGTACTACAAGATGTAGGCGGTTTTGCCTATTTGGCAGAGTTATCTAA
GAACACGCCGAGTGCGGCGAATATTCTTGCTTATGCCGATATTGTCCGTGACCGTTCGGATCTACGAGGGGTGATTTCGG
CCGGTAATCAAATTGCGGAAATGGCCTATCATACCAAAGGCAGAAGTTCAAAAGACGTGCTGGATGAAGCCGAGCGAATT
GTGTTTGAAATCGCAGAGAAACGTAATTCGGCGAATGAAGGCCCGCAAAAGATTGATGATATTTTAATTAATACGCTTGC
TCGTATCGAAATGCTTTCTAAAAATCGTCATAACGGCGGAGTAACGGGGGTTTCAACCGGTTTCCTCGATTTAAATAAAA
AAACCGCAGGTTTACAACCGTCGGATTTAATTATCGTTGCGGCGCGTCCGTCTATGGGGAAAACTACGTTTGCGATGAAC
TTATGTGAAAATGCTTCGTTACTGGATGTAGAAGATCCGAATGATTTGGACGAATACGGTAATCCTCGCAAAAAGCCGGC
GAAACCGGTATTAATTTTCAGTCTTGAGATGCCGGCGGACCAAATTATGATGCGTATGTTGGCTTCGCTTTCTCGCGTGG
ATCAAACCAAGATCCGAACCGGACAAATTCATGACGATGAAGATTGGGCGAAAATATCCAGTACGATGGCAATCTTGCAA
GAGCGCAAAAATATCTATATTGACGACAGTTCCGGTTTAACGCCGACCGAATTACGTTCTCGTGCAAGACGTGTATATCG
TGAGAACGGCGGGCTAAGTATGATCATGGTGGATTATTTGCAGTTAATGCGTGCGCCGGCGTTTTCCGATAACCGAACCC
TAGAGATTGCCGAAATTTCTCGATCTCTGAAAGCGTTGGCAAAAGAATTACAAGTGCCGGTTGTTGCACTTTCTCAGTTA
AACCGAAGTTTGGAACAACGCGCCGATAAACGACCGGTAAACTCGGATTTACGTGAATCCGGCTCTATCGAGCAAGATGC
GGACTTAATTATGTTTATTTATCGTGATGAAGTGTATAACGATAATTCCGATTTAAAAGGTATTGCCGAAATTATTATCG
GTAAGCAGCGTAACGGTCCGATTGGACGAGTGCGTTTAACTTTCCAAGGACAATATTCAAGATTTGATAACTATACAGGC
GGATCCTATGACGATGAGTACTAA

Upstream 100 bases:

>100_bases
AAATTTAAGCGTTCGATTTTCACTGAACATTTGCAAATTTCAATTTATGCAAAATTCCGTTAAACTAATTACTTATATCA
CTAACTGAAAGAGTATGAAT

Downstream 100 bases:

>100_bases
TCTCTGTGCGATTTATAAAAGCCCGAAAAGAGAAGGAATGTTTCTGTATGTGGCAAAACGAGACCAATTTGACAGTGTTC
CGGAAGCGTTACGTCAAATG

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MPEYTPTNKENSRDKQVEQIAIAPHSIEAEQAVLGGIMLNNEHWDNVSERIQAGDFYNYAHRTIFEQMVELVRHNQPIDI
ITLDQALKDKGVLQDVGGFAYLAELSKNTPSAANILAYADIVRDRSDLRGVISAGNQIAEMAYHTKGRSSKDVLDEAERI
VFEIAEKRNSANEGPQKIDDILINTLARIEMLSKNRHNGGVTGVSTGFLDLNKKTAGLQPSDLIIVAARPSMGKTTFAMN
LCENASLLDVEDPNDLDEYGNPRKKPAKPVLIFSLEMPADQIMMRMLASLSRVDQTKIRTGQIHDDEDWAKISSTMAILQ
ERKNIYIDDSSGLTPTELRSRARRVYRENGGLSMIMVDYLQLMRAPAFSDNRTLEIAEISRSLKALAKELQVPVVALSQL
NRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAEIIIGKQRNGPIGRVRLTFQGQYSRFDNYTG
GSYDDEY

Sequences:

>Translated_487_residues
MPEYTPTNKENSRDKQVEQIAIAPHSIEAEQAVLGGIMLNNEHWDNVSERIQAGDFYNYAHRTIFEQMVELVRHNQPIDI
ITLDQALKDKGVLQDVGGFAYLAELSKNTPSAANILAYADIVRDRSDLRGVISAGNQIAEMAYHTKGRSSKDVLDEAERI
VFEIAEKRNSANEGPQKIDDILINTLARIEMLSKNRHNGGVTGVSTGFLDLNKKTAGLQPSDLIIVAARPSMGKTTFAMN
LCENASLLDVEDPNDLDEYGNPRKKPAKPVLIFSLEMPADQIMMRMLASLSRVDQTKIRTGQIHDDEDWAKISSTMAILQ
ERKNIYIDDSSGLTPTELRSRARRVYRENGGLSMIMVDYLQLMRAPAFSDNRTLEIAEISRSLKALAKELQVPVVALSQL
NRSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAEIIIGKQRNGPIGRVRLTFQGQYSRFDNYTG
GSYDDEY
>Mature_486_residues
PEYTPTNKENSRDKQVEQIAIAPHSIEAEQAVLGGIMLNNEHWDNVSERIQAGDFYNYAHRTIFEQMVELVRHNQPIDII
TLDQALKDKGVLQDVGGFAYLAELSKNTPSAANILAYADIVRDRSDLRGVISAGNQIAEMAYHTKGRSSKDVLDEAERIV
FEIAEKRNSANEGPQKIDDILINTLARIEMLSKNRHNGGVTGVSTGFLDLNKKTAGLQPSDLIIVAARPSMGKTTFAMNL
CENASLLDVEDPNDLDEYGNPRKKPAKPVLIFSLEMPADQIMMRMLASLSRVDQTKIRTGQIHDDEDWAKISSTMAILQE
RKNIYIDDSSGLTPTELRSRARRVYRENGGLSMIMVDYLQLMRAPAFSDNRTLEIAEISRSLKALAKELQVPVVALSQLN
RSLEQRADKRPVNSDLRESGSIEQDADLIMFIYRDEVYNDNSDLKGIAEIIIGKQRNGPIGRVRLTFQGQYSRFDNYTGG
SYDDEY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=485, Percent_Identity=70.3092783505155, Blast_Score=670, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54646; Mature: 54515

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEYTPTNKENSRDKQVEQIAIAPHSIEAEQAVLGGIMLNNEHWDNVSERIQAGDFYNYA
CCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCEEECCCCCCCHHHHHHCCCHHHHH
HRTIFEQMVELVRHNQPIDIITLDQALKDKGVLQDVGGFAYLAELSKNTPSAANILAYAD
HHHHHHHHHHHHHCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IVRDRSDLRGVISAGNQIAEMAYHTKGRSSKDVLDEAERIVFEIAEKRNSANEGPQKIDD
HHHCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ILINTLARIEMLSKNRHNGGVTGVSTGFLDLNKKTAGLQPSDLIIVAARPSMGKTTFAMN
HHHHHHHHHHHHHCCCCCCCCCEEECCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LCENASLLDVEDPNDLDEYGNPRKKPAKPVLIFSLEMPADQIMMRMLASLSRVDQTKIRT
HHCCCCEEECCCCCCHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
GQIHDDEDWAKISSTMAILQERKNIYIDDSSGLTPTELRSRARRVYRENGGLSMIMVDYL
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHH
QLMRAPAFSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRES
HHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHC
GSIEQDADLIMFIYRDEVYNDNSDLKGIAEIIIGKQRNGPIGRVRLTFQGQYSRFDNYTG
CCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHCCCCCC
GSYDDEY
CCCCCCC
>Mature Secondary Structure 
PEYTPTNKENSRDKQVEQIAIAPHSIEAEQAVLGGIMLNNEHWDNVSERIQAGDFYNYA
CCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHCCEEECCCCCCCHHHHHHCCCHHHHH
HRTIFEQMVELVRHNQPIDIITLDQALKDKGVLQDVGGFAYLAELSKNTPSAANILAYAD
HHHHHHHHHHHHHCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
IVRDRSDLRGVISAGNQIAEMAYHTKGRSSKDVLDEAERIVFEIAEKRNSANEGPQKIDD
HHHCHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ILINTLARIEMLSKNRHNGGVTGVSTGFLDLNKKTAGLQPSDLIIVAARPSMGKTTFAMN
HHHHHHHHHHHHHCCCCCCCCCEEECCEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHH
LCENASLLDVEDPNDLDEYGNPRKKPAKPVLIFSLEMPADQIMMRMLASLSRVDQTKIRT
HHCCCCEEECCCCCCHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCC
GQIHDDEDWAKISSTMAILQERKNIYIDDSSGLTPTELRSRARRVYRENGGLSMIMVDYL
CCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEHHHHHH
QLMRAPAFSDNRTLEIAEISRSLKALAKELQVPVVALSQLNRSLEQRADKRPVNSDLRES
HHHCCCCCCCCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHC
GSIEQDADLIMFIYRDEVYNDNSDLKGIAEIIIGKQRNGPIGRVRLTFQGQYSRFDNYTG
CCCCCCCCEEEEEEEHHHCCCCCHHHHHHHHHHCCCCCCCCEEEEEEEECCHHHCCCCCC
GSYDDEY
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]