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The map label for this gene is clcA [H]

Identifier: 126208154

GI number: 126208154

Start: 769000

End: 770406

Strand: Reverse

Name: clcA [H]

Synonym: APL_0674

Alternate gene names: 126208154

Gene position: 770406-769000 (Counterclockwise)

Preceding gene: 126208156

Following gene: 126208153

Centisome position: 33.87

GC content: 46.2

Gene sequence:

>1407_bases
ATGTTTACCCGTTCTAAAACTTTCACTTTTCAATTACATCGTTTTTTGCATAGAAAAATTCACCAAACACATCAGATTTC
TCGAAAAAGTATTGAATTTGCTTGTTTGCTTATCGGAGCCGCTTTGGTGGCATTCTTTTCGTTAGGTTTTGCTAAATTAG
CCGAGTTCGGTTTACATTTGAATGCACAATGGACGGCCAAATATCCGTTAGCCGTTTGGTTTGTAATGCCGTTGGGAATG
GCCGCATTAGCTTGGTTTACTGCGAAATATACGCCTTATGTGGCGGGAAGCGGTATTCCGCAAGTGATAGCCTCAATGAG
CCTGCCGCACGGTGCGCTGAAAAGTAAATTAGTCGCATTTTGGCAAACGCTTTGGAAAATTCCGCTTACTTTTTTAGCCA
TGGTATGCGGGGCTTCCGTCGGACGGGAAGGTCCTTCCGTACAAGTCGGTGCGGCGGTAATGTTGGCTTGGGGCAATTTC
TGTCATAAACACGGCTATGCCTTTAAAGGTTTAAGTGCAAACGAGCTTATGGCGGCAGGAGCCGGCGGTGGATTAGCGGC
CGCTTTTAACGCCCCGCTTGCCGGTGTGATTTTTGCCATTGAAGAATTAGGACGAGGCGTATTATTGCGTTGGGGCAGAC
GAGTTGTTCTCGGTGTGTTAGCCTCTGGATTTATTTTAATTGCGATTGAAGGAAATAATCCGTATTTTCCCCAGTATCAA
GGTGCGTCTTCTGTCCCTAAAATGATCTTATGGGTTGCATTATGCGGTGTGGTGTGCGGAATACTGGGCGGCGTATTCGC
CCGTTTATTAGCGAAAGGCGTTGCCGGTATTTCTCCGCCTAAAATCAGAGGTTGGGTTCGTCGTCATCCAATCTATATTG
CATTCATCTTAGGGCTTATTCTTGCGGCATTAGGGCATTATACGCAAGGTCAAACTTATGGAACGGGTTATGATGTTGTT
GCAAGAGCATTAGAAGGAGAGGAAATTACCCCGCAAGCGGTCGGAATTGCCAAACTCTTTGCAACGGTCGCCACTTATTG
GACGGGTATTGCCGGCGGAATATTTACGCCGTCTTTAACAATAGGTGCGGGAATCGGCAGCGAAATATGGTTTTTGAGCG
GCGGTACGGTTGATCAGCGATTATTGGTTTTATTGTGTATGGCGGCGTTTTTGGCAGGTGCAACGCAGTCGCCGCTCAGT
GCCAGCGTCGTCGTTATGGAAATGACGGGAAGCCAACCGGTTCTTTTTTGGGTGCTGATTGCAAGCCTGATTGCCTCAAT
CGTTTCCCGCTATTTTTGTCCAAAACCTTTTTATCACTTGGCTGCCGGTCGTTTCAGACAAAGAGTACAGGAAGAAGAAA
AACGTGAGAAATCCCTTCTAAAACAAGAAGATAAAAGGATTAAATAA

Upstream 100 bases:

>100_bases
CAGGATATGACATATTTGCGCCGTTCTAAGAAAAATAAATTGAAAATCCTTGATGAATCTTTATCCTTAACAAAAATTTC
TTATATACGCGTTCCAAACT

Downstream 100 bases:

>100_bases
AAATATTATATTTTATTTAAAATATTTGGAACTTTTTTAAAACGGCTCAGTCTTAAATAACGCTAACTGACAGAATTTAT
GTAAGTTTTTTTCATTTCCT

Product: hypothetical protein

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MFTRSKTFTFQLHRFLHRKIHQTHQISRKSIEFACLLIGAALVAFFSLGFAKLAEFGLHLNAQWTAKYPLAVWFVMPLGM
AALAWFTAKYTPYVAGSGIPQVIASMSLPHGALKSKLVAFWQTLWKIPLTFLAMVCGASVGREGPSVQVGAAVMLAWGNF
CHKHGYAFKGLSANELMAAGAGGGLAAAFNAPLAGVIFAIEELGRGVLLRWGRRVVLGVLASGFILIAIEGNNPYFPQYQ
GASSVPKMILWVALCGVVCGILGGVFARLLAKGVAGISPPKIRGWVRRHPIYIAFILGLILAALGHYTQGQTYGTGYDVV
ARALEGEEITPQAVGIAKLFATVATYWTGIAGGIFTPSLTIGAGIGSEIWFLSGGTVDQRLLVLLCMAAFLAGATQSPLS
ASVVVMEMTGSQPVLFWVLIASLIASIVSRYFCPKPFYHLAAGRFRQRVQEEEKREKSLLKQEDKRIK

Sequences:

>Translated_468_residues
MFTRSKTFTFQLHRFLHRKIHQTHQISRKSIEFACLLIGAALVAFFSLGFAKLAEFGLHLNAQWTAKYPLAVWFVMPLGM
AALAWFTAKYTPYVAGSGIPQVIASMSLPHGALKSKLVAFWQTLWKIPLTFLAMVCGASVGREGPSVQVGAAVMLAWGNF
CHKHGYAFKGLSANELMAAGAGGGLAAAFNAPLAGVIFAIEELGRGVLLRWGRRVVLGVLASGFILIAIEGNNPYFPQYQ
GASSVPKMILWVALCGVVCGILGGVFARLLAKGVAGISPPKIRGWVRRHPIYIAFILGLILAALGHYTQGQTYGTGYDVV
ARALEGEEITPQAVGIAKLFATVATYWTGIAGGIFTPSLTIGAGIGSEIWFLSGGTVDQRLLVLLCMAAFLAGATQSPLS
ASVVVMEMTGSQPVLFWVLIASLIASIVSRYFCPKPFYHLAAGRFRQRVQEEEKREKSLLKQEDKRIK
>Mature_468_residues
MFTRSKTFTFQLHRFLHRKIHQTHQISRKSIEFACLLIGAALVAFFSLGFAKLAEFGLHLNAQWTAKYPLAVWFVMPLGM
AALAWFTAKYTPYVAGSGIPQVIASMSLPHGALKSKLVAFWQTLWKIPLTFLAMVCGASVGREGPSVQVGAAVMLAWGNF
CHKHGYAFKGLSANELMAAGAGGGLAAAFNAPLAGVIFAIEELGRGVLLRWGRRVVLGVLASGFILIAIEGNNPYFPQYQ
GASSVPKMILWVALCGVVCGILGGVFARLLAKGVAGISPPKIRGWVRRHPIYIAFILGLILAALGHYTQGQTYGTGYDVV
ARALEGEEITPQAVGIAKLFATVATYWTGIAGGIFTPSLTIGAGIGSEIWFLSGGTVDQRLLVLLCMAAFLAGATQSPLS
ASVVVMEMTGSQPVLFWVLIASLIASIVSRYFCPKPFYHLAAGRFRQRVQEEEKREKSLLKQEDKRIK

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786350, Length=443, Percent_Identity=27.5395033860045, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87081943, Length=259, Percent_Identity=31.2741312741313, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71985141, Length=467, Percent_Identity=24.1970021413276, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71985148, Length=467, Percent_Identity=24.1970021413276, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532615, Length=403, Percent_Identity=24.0694789081886, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24665008, Length=419, Percent_Identity=25.7756563245823, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 50510; Mature: 50510

Theoretical pI: Translated: 10.36; Mature: 10.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTRSKTFTFQLHRFLHRKIHQTHQISRKSIEFACLLIGAALVAFFSLGFAKLAEFGLHL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NAQWTAKYPLAVWFVMPLGMAALAWFTAKYTPYVAGSGIPQVIASMSLPHGALKSKLVAF
CCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
WQTLWKIPLTFLAMVCGASVGREGPSVQVGAAVMLAWGNFCHKHGYAFKGLSANELMAAG
HHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEECCCCHHHHHCCC
AGGGLAAAFNAPLAGVIFAIEELGRGVLLRWGRRVVLGVLASGFILIAIEGNNPYFPQYQ
CCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCC
GASSVPKMILWVALCGVVCGILGGVFARLLAKGVAGISPPKIRGWVRRHPIYIAFILGLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHH
LAALGHYTQGQTYGTGYDVVARALEGEEITPQAVGIAKLFATVATYWTGIAGGIFTPSLT
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
IGAGIGSEIWFLSGGTVDQRLLVLLCMAAFLAGATQSPLSASVVVMEMTGSQPVLFWVLI
EECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHH
ASLIASIVSRYFCPKPFYHLAAGRFRQRVQEEEKREKSLLKQEDKRIK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFTRSKTFTFQLHRFLHRKIHQTHQISRKSIEFACLLIGAALVAFFSLGFAKLAEFGLHL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NAQWTAKYPLAVWFVMPLGMAALAWFTAKYTPYVAGSGIPQVIASMSLPHGALKSKLVAF
CCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHH
WQTLWKIPLTFLAMVCGASVGREGPSVQVGAAVMLAWGNFCHKHGYAFKGLSANELMAAG
HHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEECCCCHHHHHCCC
AGGGLAAAFNAPLAGVIFAIEELGRGVLLRWGRRVVLGVLASGFILIAIEGNNPYFPQYQ
CCCCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCC
GASSVPKMILWVALCGVVCGILGGVFARLLAKGVAGISPPKIRGWVRRHPIYIAFILGLI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHH
LAALGHYTQGQTYGTGYDVVARALEGEEITPQAVGIAKLFATVATYWTGIAGGIFTPSLT
HHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
IGAGIGSEIWFLSGGTVDQRLLVLLCMAAFLAGATQSPLSASVVVMEMTGSQPVLFWVLI
EECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHH
ASLIASIVSRYFCPKPFYHLAAGRFRQRVQEEEKREKSLLKQEDKRIK
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA