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The map label for this gene is hlyX [H]

Identifier: 126208136

GI number: 126208136

Start: 750742

End: 751509

Strand: Direct

Name: hlyX [H]

Synonym: APL_0656

Alternate gene names: 126208136

Gene position: 750742-751509 (Clockwise)

Preceding gene: 126208130

Following gene: 126208139

Centisome position: 33.01

GC content: 40.62

Gene sequence:

>768_bases
ATGAAAATTGTATCTGACGCTAAACATACAGGCCGAACGGCTTGCACTATTCATTGCCAGAATTGCAGTATTAGCCAACT
TTGCTTACCTTTTACGTTGAGCGAACACGAATTAACTCAGCTTGACAATATTATCGAACGCAAAAAACCGGTTCAAAAAT
CTCAAATCATTTTCCAATCCGGCGATGAACTTCGTTCCATCTATGCGATTCGTTCAGGTACAATTAAAAGCTATACGATT
AGCGAAAGCGGCGAAGAACAAATTACGGCGTTCCATTTACCCGGTGATCTGGTCGGATTTGATGCGATTATGAATATGAA
ACATGTCGGTTTCGCACAAGCGCTCGAAACGTCGATGATTTGCGAGATTCCATTTGATATTTTAGACGATCTCGCCGGCA
AGATGCCTAAAATCCGTCATCAAATTATGCGTTTGATGAGTAATGAAATTAAAAGCGATCAAGAAATGATTTTATTACTT
TCAAAAATGAGTGCGGAAGAAAAGTTAGCGGCGTTTTTACATAATTTATCTCAACGTTATGCGGCACGCGGTTTTTCCGC
TCGTGAATTCCGTCTGACTATGACTCGCGGCGATATCGGCAACTATCTCGGTTTAACCATTGAAACTATCAGTCGTTTAT
TAGGACGTTTCCAGAAAAGCGGTATGATTACGGTACAAGGTAAATATATTACCATCAATCGTATGGACGAACTGACCGAT
ATGGCGGGTGTAACACGTTCACAAATTCCGGTAATACAAACGGCATAA

Upstream 100 bases:

>100_bases
AAGGTTAAATAAACCGATTACCGACTTTATTATCCTAATAAATAATGTTAAAATTTGCTTGAAATCAAACTTTTACCTAT
TTTAAAAGACGGTAGCCCTT

Downstream 100 bases:

>100_bases
GCCTTATAAAAACAAAAATACCACTATTTTCTAATAGTGGTATTTTTTTGTTCGGAACATATTTGCAAATAACTTAAGAA
ATTTAACCGCTTATTTATCT

Product: fumarate/nitrate reduction transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKIVSDAKHTGRTACTIHCQNCSISQLCLPFTLSEHELTQLDNIIERKKPVQKSQIIFQSGDELRSIYAIRSGTIKSYTI
SESGEEQITAFHLPGDLVGFDAIMNMKHVGFAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
SKMSAEEKLAAFLHNLSQRYAARGFSAREFRLTMTRGDIGNYLGLTIETISRLLGRFQKSGMITVQGKYITINRMDELTD
MAGVTRSQIPVIQTA

Sequences:

>Translated_255_residues
MKIVSDAKHTGRTACTIHCQNCSISQLCLPFTLSEHELTQLDNIIERKKPVQKSQIIFQSGDELRSIYAIRSGTIKSYTI
SESGEEQITAFHLPGDLVGFDAIMNMKHVGFAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
SKMSAEEKLAAFLHNLSQRYAARGFSAREFRLTMTRGDIGNYLGLTIETISRLLGRFQKSGMITVQGKYITINRMDELTD
MAGVTRSQIPVIQTA
>Mature_255_residues
MKIVSDAKHTGRTACTIHCQNCSISQLCLPFTLSEHELTQLDNIIERKKPVQKSQIIFQSGDELRSIYAIRSGTIKSYTI
SESGEEQITAFHLPGDLVGFDAIMNMKHVGFAQALETSMICEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLL
SKMSAEEKLAAFLHNLSQRYAARGFSAREFRLTMTRGDIGNYLGLTIETISRLLGRFQKSGMITVQGKYITINRMDELTD
MAGVTRSQIPVIQTA

Specific function: Confers a hemolytic phenotype on E.coli. May regulate, rather than mediate, hemolytic activity [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787595, Length=234, Percent_Identity=75.2136752136752, Blast_Score=379, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28652; Mature: 28652

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSDAKHTGRTACTIHCQNCSISQLCLPFTLSEHELTQLDNIIERKKPVQKSQIIFQS
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHCC
GDELRSIYAIRSGTIKSYTISESGEEQITAFHLPGDLVGFDAIMNMKHVGFAQALETSMI
CHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHE
CEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLLSKMSAEEKLAAFLHNLSQRY
EECCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
AARGFSAREFRLTMTRGDIGNYLGLTIETISRLLGRFQKSGMITVQGKYITINRMDELTD
HHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECEEEEEECHHHHHH
MAGVTRSQIPVIQTA
HHCCCHHCCCEEECC
>Mature Secondary Structure
MKIVSDAKHTGRTACTIHCQNCSISQLCLPFTLSEHELTQLDNIIERKKPVQKSQIIFQS
CCCCCCCCCCCCEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHCC
GDELRSIYAIRSGTIKSYTISESGEEQITAFHLPGDLVGFDAIMNMKHVGFAQALETSMI
CHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHE
CEIPFDILDDLAGKMPKIRHQIMRLMSNEIKSDQEMILLLSKMSAEEKLAAFLHNLSQRY
EECCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
AARGFSAREFRLTMTRGDIGNYLGLTIETISRLLGRFQKSGMITVQGKYITINRMDELTD
HHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECEEEEEECHHHHHH
MAGVTRSQIPVIQTA
HHCCCHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2198268 [H]