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The map label for this gene is cpdB [H]

Identifier: 126208128

GI number: 126208128

Start: 739513

End: 741489

Strand: Direct

Name: cpdB [H]

Synonym: APL_0646

Alternate gene names: 126208128

Gene position: 739513-741489 (Clockwise)

Preceding gene: 126208125

Following gene: 126208130

Centisome position: 32.51

GC content: 42.64

Gene sequence:

>1977_bases
ATGTTAAACAGACGACGTTTTATACAATTAGGTGCAAGTACCATGCTCATACTCGGAACGAATAAATTCGCTTTCGCAAA
AGCCGGCAATCAAGCGGATTTGCGTATTATCGCTACCACAGATATTCACGGCTTTTTAACGGATTTCGATTACTACAAAG
ACGCACCGACAGAGAAATTCGGTTTTACCCGTACGGCAAGTTTAATTGAACAGGCGAGAAAAGAAGTTAAAAATAGTGTG
TTAGTTGATAACGGCGACTTAATTCAAGGTAATCCGATTGCCGATTATCAAGCTGCCGTAGGTGCTAAACAAGGAAAAGC
CGATCCGGCAATTGCTACTTTAAATGCAATGAAATACGAAGTGGGAACACTAGGAAATCATGAGTTTAACTATGGTTTGG
ATTATTTAACCGGCGCAATGAAGCAAGCGAATCATCCTATTATCAATGCGAATATCGTTAAACCGGGAACGGACGAGCCT
TTCTTCCAACCTTATTTTATTCAAGAAAAAGCCATTATTGATACGCACGGTCAAAAACAAACGATTAAAATCGGCTATAT
CGGCTTTGTACCGCCGCAAGTGATGGTTTGGGATAAAGCAAACCTTGAAGGTAAAGTGGAAGCTCGAGATATTGTAAAAA
CCGCAGAAAAATACGTACCTATCGTGAAAGAGAAGGGGGCTGACGTCGTTATCGCCTTAGCACACACAGGACCGTCCGAT
GAACCTTATAAAGAAGGTGCGGAAAATTCGGCATTCTATTTAGCCGATGTAAAAGGCATCGATGCGGTTATTTTCGGTCA
TTCGCATCGTTTATTCCCGAATAAAGAATTTGAAAAATCACCGAATGCCGATATTACAAAAGGGACGATGAAAGGTGTTC
CGGAAAGTATGGCAGGATACTGGGGAAATAATATCAGCGTAGTTGATTTAAGCTTAGCCGAACAAAATGGCAAATGGGCG
GTAGTTGATGGTAAAGCGGCGCTTCGCCCGATTTATAATGCAGAAACTAAAAAAGCGGCGATTGAAAATCATCCGGAAAT
TGTCGCATTACTTAAAGAAACGCACGAGGCAACCCGTAAATTCGTATCCCAACCGATTGGTAAAGCAACGGATAATATGT
ACAGCTACTTGGCATTAGTACAAGACGATCCGACCATCCAAATCGTAAACCAAGCGCAAAAAGCCTATGTTGAAAATGTA
GTGGTTAAAGGACTGCCGGAACTTACCGGTTTGCCGGTATTAAGTGCCGGCGCACCGTTTAAAGCGGGCGGGCGTAAAAA
CGACCCGACGGGCTTTACCGAAGTGGATAAAGGTCAATTAACCTTCCGTAATGCGGCGGATCTCTATCTCTATCCGAATA
CGTTAGTCGTTGTAAAAGTCACCGGAGCAGAGTTGAAAGAATGGTTGGAATGTAGTGCGGGTATGTTTAAACAAATCGAC
CCAACCAATACACAACCGCAAAGTCTATTAGACTGGACCGGCTTCCGTACTTATAACTTTGACGTGATCGACGGAGTAGA
GTATCAATTTGATATTACTCAACCGGCTCGTTACGATGGCGAATGTAAATTAATTAATGAAAACTCGCACCGTGTGGTGA
ATTTAACCTTTAATGGAAAACCGGTTGATCCGAAACAGGAATTTTTAATCGCCACCAATAATTATCGTGCATATGGGGCT
AAATTCCCGGGTACCGGTGACAGCCATATCGTATTTTCCGCGCCGGATGAAAACCGTCAAGTGCTTTCCAATTACATTTC
GGCGGAAAGTAAGGAGAAAGGTCATGTCGTACCGACCGCAGATAAAAACTGGCGTATTGCACCAATCAATGCCAAAGCGA
AATTAGATATTCGTTTTGAAACTTCTCCGACTGAAAAAGCGGCGGCGTTTATTAAAGAAAAAGCGCAATATCCGATGACA
TTGGTCGGTACGGACGAAATCGGCTTTGCGGTATATCAAGTTGATTTAACGAAATAA

Upstream 100 bases:

>100_bases
GGCAAATATCATCAAACCTATAAAAAGCACTAGAGTACAAGCGGTTATTTTTCTACAATAATTTGCAATATCCAATATAT
TGATAACGTAGGAGAATACA

Downstream 100 bases:

>100_bases
TTTGCGTTATTTTAATCATTACGGAATACTCGGAAGTTTTATCTAGACCCATCCGAGTATTCCTTGGTGTTATATTTGCA
TATTAATATTCGCTCTAATT

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 658; Mature: 658

Protein sequence:

>658_residues
MLNRRRFIQLGASTMLILGTNKFAFAKAGNQADLRIIATTDIHGFLTDFDYYKDAPTEKFGFTRTASLIEQARKEVKNSV
LVDNGDLIQGNPIADYQAAVGAKQGKADPAIATLNAMKYEVGTLGNHEFNYGLDYLTGAMKQANHPIINANIVKPGTDEP
FFQPYFIQEKAIIDTHGQKQTIKIGYIGFVPPQVMVWDKANLEGKVEARDIVKTAEKYVPIVKEKGADVVIALAHTGPSD
EPYKEGAENSAFYLADVKGIDAVIFGHSHRLFPNKEFEKSPNADITKGTMKGVPESMAGYWGNNISVVDLSLAEQNGKWA
VVDGKAALRPIYNAETKKAAIENHPEIVALLKETHEATRKFVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENV
VVKGLPELTGLPVLSAGAPFKAGGRKNDPTGFTEVDKGQLTFRNAADLYLYPNTLVVVKVTGAELKEWLECSAGMFKQID
PTNTQPQSLLDWTGFRTYNFDVIDGVEYQFDITQPARYDGECKLINENSHRVVNLTFNGKPVDPKQEFLIATNNYRAYGA
KFPGTGDSHIVFSAPDENRQVLSNYISAESKEKGHVVPTADKNWRIAPINAKAKLDIRFETSPTEKAAAFIKEKAQYPMT
LVGTDEIGFAVYQVDLTK

Sequences:

>Translated_658_residues
MLNRRRFIQLGASTMLILGTNKFAFAKAGNQADLRIIATTDIHGFLTDFDYYKDAPTEKFGFTRTASLIEQARKEVKNSV
LVDNGDLIQGNPIADYQAAVGAKQGKADPAIATLNAMKYEVGTLGNHEFNYGLDYLTGAMKQANHPIINANIVKPGTDEP
FFQPYFIQEKAIIDTHGQKQTIKIGYIGFVPPQVMVWDKANLEGKVEARDIVKTAEKYVPIVKEKGADVVIALAHTGPSD
EPYKEGAENSAFYLADVKGIDAVIFGHSHRLFPNKEFEKSPNADITKGTMKGVPESMAGYWGNNISVVDLSLAEQNGKWA
VVDGKAALRPIYNAETKKAAIENHPEIVALLKETHEATRKFVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENV
VVKGLPELTGLPVLSAGAPFKAGGRKNDPTGFTEVDKGQLTFRNAADLYLYPNTLVVVKVTGAELKEWLECSAGMFKQID
PTNTQPQSLLDWTGFRTYNFDVIDGVEYQFDITQPARYDGECKLINENSHRVVNLTFNGKPVDPKQEFLIATNNYRAYGA
KFPGTGDSHIVFSAPDENRQVLSNYISAESKEKGHVVPTADKNWRIAPINAKAKLDIRFETSPTEKAAAFIKEKAQYPMT
LVGTDEIGFAVYQVDLTK
>Mature_658_residues
MLNRRRFIQLGASTMLILGTNKFAFAKAGNQADLRIIATTDIHGFLTDFDYYKDAPTEKFGFTRTASLIEQARKEVKNSV
LVDNGDLIQGNPIADYQAAVGAKQGKADPAIATLNAMKYEVGTLGNHEFNYGLDYLTGAMKQANHPIINANIVKPGTDEP
FFQPYFIQEKAIIDTHGQKQTIKIGYIGFVPPQVMVWDKANLEGKVEARDIVKTAEKYVPIVKEKGADVVIALAHTGPSD
EPYKEGAENSAFYLADVKGIDAVIFGHSHRLFPNKEFEKSPNADITKGTMKGVPESMAGYWGNNISVVDLSLAEQNGKWA
VVDGKAALRPIYNAETKKAAIENHPEIVALLKETHEATRKFVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENV
VVKGLPELTGLPVLSAGAPFKAGGRKNDPTGFTEVDKGQLTFRNAADLYLYPNTLVVVKVTGAELKEWLECSAGMFKQID
PTNTQPQSLLDWTGFRTYNFDVIDGVEYQFDITQPARYDGECKLINENSHRVVNLTFNGKPVDPKQEFLIATNNYRAYGA
KFPGTGDSHIVFSAPDENRQVLSNYISAESKEKGHVVPTADKNWRIAPINAKAKLDIRFETSPTEKAAAFIKEKAQYPMT
LVGTDEIGFAVYQVDLTK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=626, Percent_Identity=63.8977635782748, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=588, Percent_Identity=20.2380952380952, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 72581; Mature: 72581

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRRRFIQLGASTMLILGTNKFAFAKAGNQADLRIIATTDIHGFLTDFDYYKDAPTEKF
CCCCCEEEEECCCEEEEEECCCEEEEECCCCCCEEEEEEECCCHHHHCCHHHCCCCCCCC
GFTRTASLIEQARKEVKNSVLVDNGDLIQGNPIADYQAAVGAKQGKADPAIATLNAMKYE
CCHHHHHHHHHHHHHHHCCEEECCCCEEECCCCCCHHHHHCCCCCCCCCCEEEHHHHEEE
VGTLGNHEFNYGLDYLTGAMKQANHPIINANIVKPGTDEPFFQPYFIQEKAIIDTHGQKQ
ECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCEEEECCCCCE
TIKIGYIGFVPPQVMVWDKANLEGKVEARDIVKTAEKYVPIVKEKGADVVIALAHTGPSD
EEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCC
EPYKEGAENSAFYLADVKGIDAVIFGHSHRLFPNKEFEKSPNADITKGTMKGVPESMAGY
CHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHH
WGNNISVVDLSLAEQNGKWAVVDGKAALRPIYNAETKKAAIENHPEIVALLKETHEATRK
CCCCEEEEEEEEECCCCCEEEEECHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
FVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENVVVKGLPELTGLPVLSAGAPF
HHHCCCCHHHHHHEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
KAGGRKNDPTGFTEVDKGQLTFRNAADLYLYPNTLVVVKVTGAELKEWLECSAGMFKQID
CCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHHCCCCCCCCC
PTNTQPQSLLDWTGFRTYNFDVIDGVEYQFDITQPARYDGECKLINENSHRVVNLTFNGK
CCCCCHHHHHHHCCCEEEEEEEECCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCC
PVDPKQEFLIATNNYRAYGAKFPGTGDSHIVFSAPDENRQVLSNYISAESKEKGHVVPTA
CCCCCCCEEEEECCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCEECCC
DKNWRIAPINAKAKLDIRFETSPTEKAAAFIKEKAQYPMTLVGTDEIGFAVYQVDLTK
CCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECC
>Mature Secondary Structure
MLNRRRFIQLGASTMLILGTNKFAFAKAGNQADLRIIATTDIHGFLTDFDYYKDAPTEKF
CCCCCEEEEECCCEEEEEECCCEEEEECCCCCCEEEEEEECCCHHHHCCHHHCCCCCCCC
GFTRTASLIEQARKEVKNSVLVDNGDLIQGNPIADYQAAVGAKQGKADPAIATLNAMKYE
CCHHHHHHHHHHHHHHHCCEEECCCCEEECCCCCCHHHHHCCCCCCCCCCEEEHHHHEEE
VGTLGNHEFNYGLDYLTGAMKQANHPIINANIVKPGTDEPFFQPYFIQEKAIIDTHGQKQ
ECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCEEEECCCCCE
TIKIGYIGFVPPQVMVWDKANLEGKVEARDIVKTAEKYVPIVKEKGADVVIALAHTGPSD
EEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCCCEEEEEECCCCCC
EPYKEGAENSAFYLADVKGIDAVIFGHSHRLFPNKEFEKSPNADITKGTMKGVPESMAGY
CHHHCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHH
WGNNISVVDLSLAEQNGKWAVVDGKAALRPIYNAETKKAAIENHPEIVALLKETHEATRK
CCCCEEEEEEEEECCCCCEEEEECHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
FVSQPIGKATDNMYSYLALVQDDPTIQIVNQAQKAYVENVVVKGLPELTGLPVLSAGAPF
HHHCCCCHHHHHHEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCC
KAGGRKNDPTGFTEVDKGQLTFRNAADLYLYPNTLVVVKVTGAELKEWLECSAGMFKQID
CCCCCCCCCCCCCCCCCCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHHCCCCCCCCC
PTNTQPQSLLDWTGFRTYNFDVIDGVEYQFDITQPARYDGECKLINENSHRVVNLTFNGK
CCCCCHHHHHHHCCCEEEEEEEECCCEEEEECCCCCCCCCEEEEEECCCCEEEEEEECCC
PVDPKQEFLIATNNYRAYGAKFPGTGDSHIVFSAPDENRQVLSNYISAESKEKGHVVPTA
CCCCCCCEEEEECCCEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCCCCCCEECCC
DKNWRIAPINAKAKLDIRFETSPTEKAAAFIKEKAQYPMTLVGTDEIGFAVYQVDLTK
CCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]