Click here to switch to the map view.

The map label for this gene is truB

Identifier: 126208123

GI number: 126208123

Start: 733330

End: 734247

Strand: Direct

Name: truB

Synonym: APL_0641

Alternate gene names: 126208123

Gene position: 733330-734247 (Clockwise)

Preceding gene: 126208122

Following gene: 126208124

Centisome position: 32.24

GC content: 42.92

Gene sequence:

>918_bases
GTGTCCAGACCTCGTAAAAAAGGGCGTGATGTTCACGGCGTATTTTTATTAGATAAACCGCAAGGAATGTCGTCAAACGA
TATTTTGCAAAAGGTTAAACGCCTGTTTCAAGCGAATAAAGCGGGGCATACCGGCGCATTAGATCCGCTTGCGACCGGAA
TGCTTCCGATTTGTTTAGGCGAAGCGACCAAGTTTTCACAATTTTTGCTTGATTCGGACAAACGTTATCAAGTTACTGCA
AAATTAGGCAAACGCACCGATACGTCCGATGCGGAAGGTCAAGTGGTGGAAACCCGTGCGGTGAACGTAGGTGAAGCGGA
AATTATTACGGCATTGGAGCAATTTCGCGGCGATATTTTACAAGTGCCGACTATGTTTTCCGCATTAAAACATAACGGTA
AACCGCTCTATGAATATGCTCGCCAAGGCATTACGGTAGAAAGAGAAGCTCGCCCGATTACGATTTTCGAATTGCGTTTT
ATCGAATATGCCGCACCTTATCTTACGTTAGAGGTACATTGCTCGAAAGGCACTTATATTCGTACTTTAGTCGATGATTT
AGGCGAAGTATTGGGCTGTGGTGCGCACGTGACGGTTCTACGCCGTTTAGCGGTGGCAAATTATCCGATTGAAGCGATGA
TGAGCTATGCGGATTTGCAAAATTTAAGTGAAAATCAACCGCTTGAAGAATTAGATAAATATCTTTTACCTTTAGATACC
GCAGTAGAAAGCCTACCAAAACTAAACTTAACCGCAGAGCAGACTAAAGCGGTCGGTTTCGGTCAAAGAGTGAAATTTGA
AAATAACGATCAAATTTACGGACAAGTTCGTTTATTTTCGCATAATATGCAATTCCTCGGTGTCGCGGAAATTACGACCG
ATAATGTCATTCGCCCAAGCAGAATGGTAAATATTTAA

Upstream 100 bases:

>100_bases
TTTTATTTACGATCAATCCTTAGTCGAAGGTATGCGTATGTCTAACCTTGTCTCGAACGTGATTAAAAACGACGAAGCTA
AACATAAAGAGGAAGAATAG

Downstream 100 bases:

>100_bases
TTTACAAGCGGTCGGTTTTAAAGATCGCTTTACTATATAAATGCTATGAAATTACGATTTTTAACGATTCCGGTAATGTT
TAGCTTATTCCTTGCCGGAT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSRPRKKGRDVHGVFLLDKPQGMSSNDILQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYQVTA
KLGKRTDTSDAEGQVVETRAVNVGEAEIITALEQFRGDILQVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELRF
IEYAAPYLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTVLRRLAVANYPIEAMMSYADLQNLSENQPLEELDKYLLPLDT
AVESLPKLNLTAEQTKAVGFGQRVKFENNDQIYGQVRLFSHNMQFLGVAEITTDNVIRPSRMVNI

Sequences:

>Translated_305_residues
MSRPRKKGRDVHGVFLLDKPQGMSSNDILQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYQVTA
KLGKRTDTSDAEGQVVETRAVNVGEAEIITALEQFRGDILQVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELRF
IEYAAPYLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTVLRRLAVANYPIEAMMSYADLQNLSENQPLEELDKYLLPLDT
AVESLPKLNLTAEQTKAVGFGQRVKFENNDQIYGQVRLFSHNMQFLGVAEITTDNVIRPSRMVNI
>Mature_304_residues
SRPRKKGRDVHGVFLLDKPQGMSSNDILQKVKRLFQANKAGHTGALDPLATGMLPICLGEATKFSQFLLDSDKRYQVTAK
LGKRTDTSDAEGQVVETRAVNVGEAEIITALEQFRGDILQVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELRFI
EYAAPYLTLEVHCSKGTYIRTLVDDLGEVLGCGAHVTVLRRLAVANYPIEAMMSYADLQNLSENQPLEELDKYLLPLDTA
VESLPKLNLTAEQTKAVGFGQRVKFENNDQIYGQVRLFSHNMQFLGVAEITTDNVIRPSRMVNI

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=219, Percent_Identity=35.1598173515982, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI4503337, Length=260, Percent_Identity=28.0769230769231, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI215599015, Length=260, Percent_Identity=28.0769230769231, Blast_Score=78, Evalue=9e-15,
Organism=Escherichia coli, GI2367200, Length=310, Percent_Identity=58.0645161290323, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17553978, Length=290, Percent_Identity=25.5172413793103, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=290, Percent_Identity=26.551724137931, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=190, Percent_Identity=30.5263157894737, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281364189, Length=259, Percent_Identity=27.027027027027, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364187, Length=259, Percent_Identity=27.027027027027, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364185, Length=259, Percent_Identity=27.027027027027, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281364183, Length=259, Percent_Identity=27.027027027027, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI62471759, Length=259, Percent_Identity=27.027027027027, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI17975520, Length=259, Percent_Identity=27.027027027027, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_ACTP2 (A3N007)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053348.1
- ProteinModelPortal:   A3N007
- SMR:   A3N007
- STRING:   A3N007
- GeneID:   4848654
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0641
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- PhylomeDB:   A3N007
- ProtClustDB:   PRK05033
- BioCyc:   APLE416269:APL_0641-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34090; Mature: 33958

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPRKKGRDVHGVFLLDKPQGMSSNDILQKVKRLFQANKAGHTGALDPLATGMLPICLG
CCCCCHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDSDKRYQVTAKLGKRTDTSDAEGQVVETRAVNVGEAEIITALEQFRGDIL
CHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHH
QVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELRFIEYAAPYLTLEVHCSKGTYI
HHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEEEEEHHHHHCCCEEEEEEEECCCCCH
RTLVDDLGEVLGCGAHVTVLRRLAVANYPIEAMMSYADLQNLSENQPLEELDKYLLPLDT
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHH
AVESLPKLNLTAEQTKAVGFGQRVKFENNDQIYGQVRLFSHNMQFLGVAEITTDNVIRPS
HHHHCCCCCCCHHHHHHCCCCCEEEECCCCEEEEEEEEEECCCEEEEEEEECCCCCCCCH
RMVNI
HHCCC
>Mature Secondary Structure 
SRPRKKGRDVHGVFLLDKPQGMSSNDILQKVKRLFQANKAGHTGALDPLATGMLPICLG
CCCCHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHC
EATKFSQFLLDSDKRYQVTAKLGKRTDTSDAEGQVVETRAVNVGEAEIITALEQFRGDIL
CHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCHH
QVPTMFSALKHNGKPLYEYARQGITVEREARPITIFELRFIEYAAPYLTLEVHCSKGTYI
HHHHHHHHHHCCCCHHHHHHHCCCEEECCCCCEEEEEEHHHHHCCCEEEEEEEECCCCCH
RTLVDDLGEVLGCGAHVTVLRRLAVANYPIEAMMSYADLQNLSENQPLEELDKYLLPLDT
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHH
AVESLPKLNLTAEQTKAVGFGQRVKFENNDQIYGQVRLFSHNMQFLGVAEITTDNVIRPS
HHHHCCCCCCCHHHHHHCCCCCEEEECCCCEEEEEEEEEECCCEEEEEEEECCCCCCCCH
RMVNI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA