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The map label for this gene is tsf

Identifier: 126208049

GI number: 126208049

Start: 639615

End: 640466

Strand: Direct

Name: tsf

Synonym: APL_0567

Alternate gene names: 126208049

Gene position: 639615-640466 (Clockwise)

Preceding gene: 126208048

Following gene: 126208051

Centisome position: 28.12

GC content: 43.54

Gene sequence:

>852_bases
ATGGCTGAAATCACAGCATCACTCGTAAAAGAACTTCGTGAGCGTACCGGCGCAGGTATGATGGAATGTAAAAAAGCGTT
AGTTGAAGCAAACGGTGATATCGAATTAGCAATCGACAATATGCGTAAATCTGGTCAAGCTAAAGCGGCTAAAAAAGCAG
GTCGTGTGGCGGCAGAAGGCGTTATCTTAGCTCGTATCGGCGCAGGCTTCGGCGTATTAGTTGAAATGAACTGCGAAACC
GACTTCGTAGCGAAAGACGCAGGTTTCTTAGGTTTAGCAAACGAAGTTGCTGATTTCGCATTAGCAAACAAAGGTACAAC
GATTGAAACGTTAGCGGCACAATTCGAAGAAAAACGTGCGGCGTTAGTGGCTAAAATCGGTGAGAACATGAACATTCGTC
GTGTTCAATACTTAGACGGTCAAGTTATCGCTCAATACTTACACGGTGCGAAAATCGGTGTATTAGTAGCGGGTGAAGGT
TCGGACGAAGAATTGAAAAAAGTTGCAATGCACGTTGCGGCAAGCCGTCCTGAATTCGTAAACCCTGAAGATGTTTCTGC
AGAAGTTGTTGCACACGAACGCCAAATCCAAATCGACATCGCTATCAACTCTGGTAAACCGAAAGAAATCGCAGAGAAAA
TGGTTGAAGGTCGTATGAAGAAATTCACCGGTGAAGTTTCATTAACAGGTCAAGCGTTTGTAATGGATCCTTCACAATCA
GTAGGCGACTACTTAAAATCTGTAAATACGAAAGTAACTAACTTCATCCGTTTAGAAGTTGGTGAAGGTATCGAGAAAGT
TGAAGAAGATTTCGCAGCGGAAGTTGCTAAAATTACCGGCGGTAACGCTTAA

Upstream 100 bases:

>100_bases
AATAATCGTTATTAAGGCATAGCCCTTAAATCACTTTGGAGCAGGGGCGATTTTTATCCCCCTGCTTTTCTTATCTAATT
CAACCTTAGAGGAATACAAA

Downstream 100 bases:

>100_bases
TTCTCAATTTAATAAAAAAGCAGGCGATTAGCCTGCTTTTTTTCTGTCTGTTGCTATGCAAGCGGTCATTTTTCTTCGGA
TTTTTGCAAATAGCATACTA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEGVILARIGAGFGVLVEMNCET
DFVAKDAGFLGLANEVADFALANKGTTIETLAAQFEEKRAALVAKIGENMNIRRVQYLDGQVIAQYLHGAKIGVLVAGEG
SDEELKKVAMHVAASRPEFVNPEDVSAEVVAHERQIQIDIAINSGKPKEIAEKMVEGRMKKFTGEVSLTGQAFVMDPSQS
VGDYLKSVNTKVTNFIRLEVGEGIEKVEEDFAAEVAKITGGNA

Sequences:

>Translated_283_residues
MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEGVILARIGAGFGVLVEMNCET
DFVAKDAGFLGLANEVADFALANKGTTIETLAAQFEEKRAALVAKIGENMNIRRVQYLDGQVIAQYLHGAKIGVLVAGEG
SDEELKKVAMHVAASRPEFVNPEDVSAEVVAHERQIQIDIAINSGKPKEIAEKMVEGRMKKFTGEVSLTGQAFVMDPSQS
VGDYLKSVNTKVTNFIRLEVGEGIEKVEEDFAAEVAKITGGNA
>Mature_282_residues
AEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEGVILARIGAGFGVLVEMNCETD
FVAKDAGFLGLANEVADFALANKGTTIETLAAQFEEKRAALVAKIGENMNIRRVQYLDGQVIAQYLHGAKIGVLVAGEGS
DEELKKVAMHVAASRPEFVNPEDVSAEVVAHERQIQIDIAINSGKPKEIAEKMVEGRMKKFTGEVSLTGQAFVMDPSQSV
GDYLKSVNTKVTNFIRLEVGEGIEKVEEDFAAEVAKITGGNA

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Escherichia coli, GI1786366, Length=280, Percent_Identity=72.5, Blast_Score=402, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17561440, Length=292, Percent_Identity=25, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI19921466, Length=289, Percent_Identity=29.4117647058824, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_ACTP2 (A3MZT3)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053274.1
- ProteinModelPortal:   A3MZT3
- SMR:   A3MZT3
- STRING:   A3MZT3
- GeneID:   4848776
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0567
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- PhylomeDB:   A3MZT3
- ProtClustDB:   PRK09377
- BioCyc:   APLE416269:APL_0567-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30242; Mature: 30111

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHCC
VILARIGAGFGVLVEMNCETDFVAKDAGFLGLANEVADFALANKGTTIETLAAQFEEKRA
EEEEEECCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALVAKIGENMNIRRVQYLDGQVIAQYLHGAKIGVLVAGEGSDEELKKVAMHVAASRPEFV
HHHHHHCCCCCEEEEEHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
NPEDVSAEVVAHERQIQIDIAINSGKPKEIAEKMVEGRMKKFTGEVSLTGQAFVMDPSQS
CCCCCCHHHEECCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCEEEEECCCHH
VGDYLKSVNTKVTNFIRLEVGEGIEKVEEDFAAEVAKITGGNA
HHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHCC
VILARIGAGFGVLVEMNCETDFVAKDAGFLGLANEVADFALANKGTTIETLAAQFEEKRA
EEEEEECCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
ALVAKIGENMNIRRVQYLDGQVIAQYLHGAKIGVLVAGEGSDEELKKVAMHVAASRPEFV
HHHHHHCCCCCEEEEEHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCC
NPEDVSAEVVAHERQIQIDIAINSGKPKEIAEKMVEGRMKKFTGEVSLTGQAFVMDPSQS
CCCCCCHHHEECCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCEEEEECCCHH
VGDYLKSVNTKVTNFIRLEVGEGIEKVEEDFAAEVAKITGGNA
HHHHHHHHHHHHHHEEEEECCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA