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The map label for this gene is yaaJ [C]

Identifier: 126208012

GI number: 126208012

Start: 601844

End: 603292

Strand: Direct

Name: yaaJ [C]

Synonym: APL_0528

Alternate gene names: 126208012

Gene position: 601844-603292 (Clockwise)

Preceding gene: 126208011

Following gene: 126208013

Centisome position: 26.46

GC content: 44.38

Gene sequence:

>1449_bases
ATGGATTTTATTGTTTCGGGTTTTGAAAATGTCCTGACATGGATTGTGAATACGATTGACGGTCCGCTTTGGGACATCAC
TATTTTAATCCTTTTAGGAACGGGGCTGTTTTTTACGATTACCACCGGCTTTGTTCAACTGCGTTTATTACCTCGTAGTT
TACGTGAAATGTGGGGCGGACGTTCAGCGGAAGGGAAATCTTTAACGCCTTTCCAAGCGTTTACAACCGGTTTAGCCAGC
CGTGTCGGTGTCGGTAATATCGGTGGGGTGGCAACCGCTATCGCCTTAGGCGGTCCGGGTGCGGTATTCTGGATGTGGAT
TACCGCTTTAATGGGAATGAGTTCTGCATTTGCGGAATCTTCGCTTGCCCAACTATATAAAACGCGTGATCCGAACGGTA
TGTTCCGCGGCGGCCCTGCATACTATATTACGCGCGGTTTGAAATTTAAACCGATGGCGATTGCATTTGCGATTGCATTA
ATTTTTACCTTCGGTTTTGCTTTTAATGCGGTGCAATCCAACTCGATTGTGGCGGCGACCAGTAAAGCATGGGAATGGGA
CGGACAATATATCGGTTTAATATTGGTAATTGTAACCGGTATTATTATTTCCGGCGGGGTAAAACTTATCGGGCAAGTTT
CCGCTAAAATCGTGCCGATGATGGCATTATTTTATCTGATCATTGCGGTCATTATTTTAGGTATGAATATCGAACGTGTT
CCGGCGGTATTCGGATTAATTATTGACAGTGCGTTTGACTTTTCAGCGGCTGCAGGCGGTATGTTCGGTGCTTTAGTGTC
GAAAACGATGTTGATGGGGATTAAGCGCGGTTTATTCTCAAATGAAGCCGGTATGGGTTCCGCACCGAATTCAGCGGCGA
CGTCCGATGCAAAACATCCGGCAAGCCAAGGTTTAATCCAAATGTTAGGCGTATTTGTCGATACTATGGTGGTTTGTACC
TGTACGGCAGTCATTATTTTAATGTCTGATAATTATGGCGGTGATTCTTTAAAAGGCGTTGAACTCACTCAGACCGCGTT
ACAATACCATTTAGGCGAGTTCGGCGTACATTTCTTAGCGTTTATTTTGTTATTATTCTGCTACACCTCAATCATCGGTA
ACTATGCTTATGCGGAAACCAATATTCGTTATATCAAAAACAAACCTTGGTTTGTGTGGGCTTTCCGTGCGGTTGTGTTG
TTCTTCGTCTATTTCGGTGCGGTGCGTGACGGCGGTATCGTTTGGGCGTTTGCCGATACGGTAATGGCTTCCATGGCGGT
GATTAACTTAATTGCAATCTTGATTCTTGCGCCAATCGTATGGCGTTTATTAAAAGATTATGTGCGTCAAGTCAAAGCGG
GACAAGAGCCGGTCTTTAAGATTGAAGAACATCAAGATTTAATTCATCGCGGTGTGGATCCGTTAATTTGGAAGTCTAAA
GAAGATTAA

Upstream 100 bases:

>100_bases
GATTTTACTAATGGAAAGATTAAAGATCTTTTCAAAAGTTAAAAAATCATTAAAATGCAAACGTTTTCGTTTTTATTTTT
CATACTATAAGAGGTCTTTG

Downstream 100 bases:

>100_bases
TAAATAACCTATTAGCATTAGCTAAAGAAGCGATAATATCTTATGGTGTTATCGCTTTTTTATATCCGGCGATAAGTCCG
GATTTTGTTAATTTTGAATA

Product: putative transporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MDFIVSGFENVLTWIVNTIDGPLWDITILILLGTGLFFTITTGFVQLRLLPRSLREMWGGRSAEGKSLTPFQAFTTGLAS
RVGVGNIGGVATAIALGGPGAVFWMWITALMGMSSAFAESSLAQLYKTRDPNGMFRGGPAYYITRGLKFKPMAIAFAIAL
IFTFGFAFNAVQSNSIVAATSKAWEWDGQYIGLILVIVTGIIISGGVKLIGQVSAKIVPMMALFYLIIAVIILGMNIERV
PAVFGLIIDSAFDFSAAAGGMFGALVSKTMLMGIKRGLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTMVVCT
CTAVIILMSDNYGGDSLKGVELTQTALQYHLGEFGVHFLAFILLLFCYTSIIGNYAYAETNIRYIKNKPWFVWAFRAVVL
FFVYFGAVRDGGIVWAFADTVMASMAVINLIAILILAPIVWRLLKDYVRQVKAGQEPVFKIEEHQDLIHRGVDPLIWKSK
ED

Sequences:

>Translated_482_residues
MDFIVSGFENVLTWIVNTIDGPLWDITILILLGTGLFFTITTGFVQLRLLPRSLREMWGGRSAEGKSLTPFQAFTTGLAS
RVGVGNIGGVATAIALGGPGAVFWMWITALMGMSSAFAESSLAQLYKTRDPNGMFRGGPAYYITRGLKFKPMAIAFAIAL
IFTFGFAFNAVQSNSIVAATSKAWEWDGQYIGLILVIVTGIIISGGVKLIGQVSAKIVPMMALFYLIIAVIILGMNIERV
PAVFGLIIDSAFDFSAAAGGMFGALVSKTMLMGIKRGLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTMVVCT
CTAVIILMSDNYGGDSLKGVELTQTALQYHLGEFGVHFLAFILLLFCYTSIIGNYAYAETNIRYIKNKPWFVWAFRAVVL
FFVYFGAVRDGGIVWAFADTVMASMAVINLIAILILAPIVWRLLKDYVRQVKAGQEPVFKIEEHQDLIHRGVDPLIWKSK
ED
>Mature_482_residues
MDFIVSGFENVLTWIVNTIDGPLWDITILILLGTGLFFTITTGFVQLRLLPRSLREMWGGRSAEGKSLTPFQAFTTGLAS
RVGVGNIGGVATAIALGGPGAVFWMWITALMGMSSAFAESSLAQLYKTRDPNGMFRGGPAYYITRGLKFKPMAIAFAIAL
IFTFGFAFNAVQSNSIVAATSKAWEWDGQYIGLILVIVTGIIISGGVKLIGQVSAKIVPMMALFYLIIAVIILGMNIERV
PAVFGLIIDSAFDFSAAAGGMFGALVSKTMLMGIKRGLFSNEAGMGSAPNSAATSDAKHPASQGLIQMLGVFVDTMVVCT
CTAVIILMSDNYGGDSLKGVELTQTALQYHLGEFGVHFLAFILLLFCYTSIIGNYAYAETNIRYIKNKPWFVWAFRAVVL
FFVYFGAVRDGGIVWAFADTVMASMAVINLIAILILAPIVWRLLKDYVRQVKAGQEPVFKIEEHQDLIHRGVDPLIWKSK
ED

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=468, Percent_Identity=41.025641025641, Blast_Score=345, Evalue=5e-96,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 52331; Mature: 52331

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFIVSGFENVLTWIVNTIDGPLWDITILILLGTGLFFTITTGFVQLRLLPRSLREMWGG
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
RSAEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAVFWMWITALMGMSSAFAES
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHH
SLAQLYKTRDPNGMFRGGPAYYITRGLKFKPMAIAFAIALIFTFGFAFNAVQSNSIVAAT
HHHHHHHCCCCCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
SKAWEWDGQYIGLILVIVTGIIISGGVKLIGQVSAKIVPMMALFYLIIAVIILGMNIERV
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
PAVFGLIIDSAFDFSAAAGGMFGALVSKTMLMGIKRGLFSNEAGMGSAPNSAATSDAKHP
HHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
ASQGLIQMLGVFVDTMVVCTCTAVIILMSDNYGGDSLKGVELTQTALQYHLGEFGVHFLA
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FILLLFCYTSIIGNYAYAETNIRYIKNKPWFVWAFRAVVLFFVYFGAVRDGGIVWAFADT
HHHHHHHHHHHHCCCCEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHH
VMASMAVINLIAILILAPIVWRLLKDYVRQVKAGQEPVFKIEEHQDLIHRGVDPLIWKSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCCCC
ED
CC
>Mature Secondary Structure
MDFIVSGFENVLTWIVNTIDGPLWDITILILLGTGLFFTITTGFVQLRLLPRSLREMWGG
CCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
RSAEGKSLTPFQAFTTGLASRVGVGNIGGVATAIALGGPGAVFWMWITALMGMSSAFAES
CCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHH
SLAQLYKTRDPNGMFRGGPAYYITRGLKFKPMAIAFAIALIFTFGFAFNAVQSNSIVAAT
HHHHHHHCCCCCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEC
SKAWEWDGQYIGLILVIVTGIIISGGVKLIGQVSAKIVPMMALFYLIIAVIILGMNIERV
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
PAVFGLIIDSAFDFSAAAGGMFGALVSKTMLMGIKRGLFSNEAGMGSAPNSAATSDAKHP
HHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
ASQGLIQMLGVFVDTMVVCTCTAVIILMSDNYGGDSLKGVELTQTALQYHLGEFGVHFLA
HHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
FILLLFCYTSIIGNYAYAETNIRYIKNKPWFVWAFRAVVLFFVYFGAVRDGGIVWAFADT
HHHHHHHHHHHHCCCCEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHH
VMASMAVINLIAILILAPIVWRLLKDYVRQVKAGQEPVFKIEEHQDLIHRGVDPLIWKSK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCCCCCC
ED
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]