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The map label for this gene is macA [H]

Identifier: 126207875

GI number: 126207875

Start: 444512

End: 445693

Strand: Reverse

Name: macA [H]

Synonym: APL_0391

Alternate gene names: 126207875

Gene position: 445693-444512 (Counterclockwise)

Preceding gene: 126207876

Following gene: 126207873

Centisome position: 19.6

GC content: 43.57

Gene sequence:

>1182_bases
ATGAAATTAAAATCGTTAATCACGGGATTGACCCTTGCCGGTATTATAACCGCCGGCTATTTCTATTTTTTTAATCAAAG
TGACAATCAACAAGTCTATTATATTACCGAACCCGTCGCACGAGGCACGATTGATAAGCATGTATTGGCAACCGGCTCGG
TACGCGCCAGCAAGCGAACCGAAGTCGGGGCGCAGGTATCCGGTAAAATCATTAATCTTTATGTGACCTTGGGGCAACAA
GTTAAAAAAGGCGATTTGCTTGCCGAAATAGATTCGAGTAATCAAAGTAACAGCTTAAGCACGGCGGAAGCAACGCTTTC
CTCTTATCAGGCACAGTTAAAATCCGCTCAAGTCGCATTGGAAGTCGCACAATCCAATTTCAATCGTTTAAACAAACTTT
ATAAGCAACAAAGCGCTTCCCAAAGTGATTTTGAAACGGCGAAAAATACCTTAGCTTCCGCCAGAGCGACGGTAGATAAC
GTAAAAGCGCAAATTAAATCGGCACAAATTTCGGTAAACGACGCTAAAACCAATTTAAATTATACGCAAATCGTATCGCC
GATGGACGGCACTATTGTGTCCATACCGGTTTCGGTCGGGCAAACCGTCAATGCGAATCAAACCTCGCCGACTATCGTTC
ATGTGGCTGATTTAAGCAAGATGCTAGTCAAACTTGAGATTTCCGAAGGCGATATCGCTCAAGTAAAAGCGGGACAAGCG
ATTCGATTTACGACACTGGCGGAACCTAATCATACGCACAACAGTAAAATTGACAGCGTAGATCCGGCATTAACTACCTT
AAGTGACGATAACGCCTCAAATAAATATGCGGAGAAATCAGGCAATACCGAAGCGATTTACTACTACGCCAATTCTTTGG
TTGAAAATTCGGAGCAAAGATTACGTATCGGTATGACGGTGCAAGGGCAAGTGGATATTGTTAAGCGCGAAAACGTGTTG
ATCGTGCCGACATCTGCGCTGAAGAAAAAAAGTAATGCGGTGTTTATCAATGTATTGGAAAATAATCAATCGATTGAGAA
AAAAGTCGAAATCGGGCTTGCCGACAGTCAATATACCGAAGTGTTGAGCGGTTTAAATGAGGGAGAAAAAGTGATTACCG
CCCAACGTACGGATGATGAAAAAATCAGTGCGGAAATGCGCCGCAGACCGGGCGGATTCTAA

Upstream 100 bases:

>100_bases
CTGATCGTTTATCTATTAACAAGCGGTCTGATAACCGAACGATATTGCAAATTTTTGGTCTTAACAGACCGCATATTACC
TAGACATTATCGGAAAACAA

Downstream 100 bases:

>100_bases
TTGACTAAAAGCAAGCGGTGGAAATTTACAATATTTTAGTAAATTTCCACCGCTTGTTTACATTAAACCGTCCGAGAAAT
TATTTTTCGTTTTTAATCGA

Product: macrolide-specific efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MKLKSLITGLTLAGIITAGYFYFFNQSDNQQVYYITEPVARGTIDKHVLATGSVRASKRTEVGAQVSGKIINLYVTLGQQ
VKKGDLLAEIDSSNQSNSLSTAEATLSSYQAQLKSAQVALEVAQSNFNRLNKLYKQQSASQSDFETAKNTLASARATVDN
VKAQIKSAQISVNDAKTNLNYTQIVSPMDGTIVSIPVSVGQTVNANQTSPTIVHVADLSKMLVKLEISEGDIAQVKAGQA
IRFTTLAEPNHTHNSKIDSVDPALTTLSDDNASNKYAEKSGNTEAIYYYANSLVENSEQRLRIGMTVQGQVDIVKRENVL
IVPTSALKKKSNAVFINVLENNQSIEKKVEIGLADSQYTEVLSGLNEGEKVITAQRTDDEKISAEMRRRPGGF

Sequences:

>Translated_393_residues
MKLKSLITGLTLAGIITAGYFYFFNQSDNQQVYYITEPVARGTIDKHVLATGSVRASKRTEVGAQVSGKIINLYVTLGQQ
VKKGDLLAEIDSSNQSNSLSTAEATLSSYQAQLKSAQVALEVAQSNFNRLNKLYKQQSASQSDFETAKNTLASARATVDN
VKAQIKSAQISVNDAKTNLNYTQIVSPMDGTIVSIPVSVGQTVNANQTSPTIVHVADLSKMLVKLEISEGDIAQVKAGQA
IRFTTLAEPNHTHNSKIDSVDPALTTLSDDNASNKYAEKSGNTEAIYYYANSLVENSEQRLRIGMTVQGQVDIVKRENVL
IVPTSALKKKSNAVFINVLENNQSIEKKVEIGLADSQYTEVLSGLNEGEKVITAQRTDDEKISAEMRRRPGGF
>Mature_393_residues
MKLKSLITGLTLAGIITAGYFYFFNQSDNQQVYYITEPVARGTIDKHVLATGSVRASKRTEVGAQVSGKIINLYVTLGQQ
VKKGDLLAEIDSSNQSNSLSTAEATLSSYQAQLKSAQVALEVAQSNFNRLNKLYKQQSASQSDFETAKNTLASARATVDN
VKAQIKSAQISVNDAKTNLNYTQIVSPMDGTIVSIPVSVGQTVNANQTSPTIVHVADLSKMLVKLEISEGDIAQVKAGQA
IRFTTLAEPNHTHNSKIDSVDPALTTLSDDNASNKYAEKSGNTEAIYYYANSLVENSEQRLRIGMTVQGQVDIVKRENVL
IVPTSALKKKSNAVFINVLENNQSIEKKVEIGLADSQYTEVLSGLNEGEKVITAQRTDDEKISAEMRRRPGGF

Specific function: Efflux transporter for macrolide antibiotics [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=363, Percent_Identity=35.2617079889807, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI87082036, Length=335, Percent_Identity=27.4626865671642, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1789929, Length=348, Percent_Identity=26.4367816091954, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790519, Length=249, Percent_Identity=25.3012048192771, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42675; Mature: 42675

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKSLITGLTLAGIITAGYFYFFNQSDNQQVYYITEPVARGTIDKHVLATGSVRASKRT
CCHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEECCHHCCCCCHHEEECCCCCCCCHH
EVGAQVSGKIINLYVTLGQQVKKGDLLAEIDSSNQSNSLSTAEATLSSYQAQLKSAQVAL
CCCCEECCEEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EVAQSNFNRLNKLYKQQSASQSDFETAKNTLASARATVDNVKAQIKSAQISVNDAKTNLN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCC
YTQIVSPMDGTIVSIPVSVGQTVNANQTSPTIVHVADLSKMLVKLEISEGDIAQVKAGQA
HHEEECCCCCEEEEEEEECCCEECCCCCCCCEEEHHHHHEEEEEEECCCCCEEEECCCCE
IRFTTLAEPNHTHNSKIDSVDPALTTLSDDNASNKYAEKSGNTEAIYYYANSLVENSEQR
EEEEEECCCCCCCCCCCCCCCCHHEEECCCCCCCCHHHCCCCCCEEEEEEHHHHHCCCCE
LRIGMTVQGQVDIVKRENVLIVPTSALKKKSNAVFINVLENNQSIEKKVEIGLADSQYTE
EEEEEEEECCEEEEECCCEEEEECHHHHCCCCEEEEEEECCCCCCHHHEEECCCCHHHHH
VLSGLNEGEKVITAQRTDDEKISAEMRRRPGGF
HHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLKSLITGLTLAGIITAGYFYFFNQSDNQQVYYITEPVARGTIDKHVLATGSVRASKRT
CCHHHHHHHHHHHHHHHHCEEEEEECCCCCEEEEEECCHHCCCCCHHEEECCCCCCCCHH
EVGAQVSGKIINLYVTLGQQVKKGDLLAEIDSSNQSNSLSTAEATLSSYQAQLKSAQVAL
CCCCEECCEEEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EVAQSNFNRLNKLYKQQSASQSDFETAKNTLASARATVDNVKAQIKSAQISVNDAKTNLN
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCC
YTQIVSPMDGTIVSIPVSVGQTVNANQTSPTIVHVADLSKMLVKLEISEGDIAQVKAGQA
HHEEECCCCCEEEEEEEECCCEECCCCCCCCEEEHHHHHEEEEEEECCCCCEEEECCCCE
IRFTTLAEPNHTHNSKIDSVDPALTTLSDDNASNKYAEKSGNTEAIYYYANSLVENSEQR
EEEEEECCCCCCCCCCCCCCCCHHEEECCCCCCCCHHHCCCCCCEEEEEEHHHHHCCCCE
LRIGMTVQGQVDIVKRENVLIVPTSALKKKSNAVFINVLENNQSIEKKVEIGLADSQYTE
EEEEEEEECCEEEEECCCEEEEECHHHHCCCCEEEEEEECCCCCCHHHEEECCCCHHHHH
VLSGLNEGEKVITAQRTDDEKISAEMRRRPGGF
HHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]