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The map label for this gene is rph [H]

Identifier: 126207543

GI number: 126207543

Start: 61124

End: 61840

Strand: Reverse

Name: rph [H]

Synonym: APL_0055

Alternate gene names: 126207543

Gene position: 61840-61124 (Counterclockwise)

Preceding gene: 126207546

Following gene: 126207539

Centisome position: 2.72

GC content: 46.44

Gene sequence:

>717_bases
ATGCGCCCAAATAATCGTGAACTGAATCAAGTTCGTCCGGTAAAAATTACTCGTCACTATACTCGTTATGCGGAAGGTTC
CGTTTTAGTTGAATTCGGCGAAACCAAAGTTTTATGTAATGCTACCGTGGAAGAAACGGTTCCTCGCTTTTTAAAAGGTC
AGCAGCAAGGCTGGGTAACCGCCGAATACGGTATGTTGCCACGTTCAACCCACAGCCGTATGCAACGTGAAGCGGCAAAA
GGCAAACAAGGCGGCAGAACCATGGAAATTCAACGTCTTATCGCACGCTCATTACGTGCAGTAGTCGATTTAAAAGCACT
TGGCGAACGTACGGTTACGGTTGATTGTGATGTGATTCAAGCGGACGGCGGTACTCGTACGGCGGCAATTACCGGTGCGT
GTGTGGCTTTACACGATGCGATGAGTAAATTAGTCGCTGACGGCGTACTGAAAGAAAATCCGATGAAAGGTTTAGTGGCG
GCAATTTCCGTCGGTATCGTGGACGGTAATGCGGTATGTGATTTGGAATATGTCGAAGATTCAAATGCCGAAACGGATAT
GAATGTAGTGATGGTCGAAGACGGCAGATTAGTCGAAGTGCAAGGCACTGCGGAAGGCGAGCCGTTCTCACATATGGAAT
TGTTACAATTACTCGATCTCGCTCATCAAGGCATTAATCAATTGTTAGATGCTCAACGCAAAGCATTGGCTTTATAA

Upstream 100 bases:

>100_bases
ACCTTTCCGTGTATTTCCGTATTTTCCGAGGCAATAAAAATCACATTGTACTGTTTTATTTGTTAAAATGGCGAAAATTT
TTTAATAAAAGGAAGAAATC

Downstream 100 bases:

>100_bases
AAATAAACGGCATAAGTAAATTAAAATTAAATACTTGTGCCGTTTTTGTTTCTAACGATTGGTAATTAAACTTCAATACC
AAATTTTTCTTTCACTAACG

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPNNRELNQVRPVKITRHYTRYAEGSVLVEFGETKVLCNATVEETVPRFLKGQQQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTMEIQRLIARSLRAVVDLKALGERTVTVDCDVIQADGGTRTAAITGACVALHDAMSKLVADGVLKENPMKGLVA
AISVGIVDGNAVCDLEYVEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHMELLQLLDLAHQGINQLLDAQRKALAL

Sequences:

>Translated_238_residues
MRPNNRELNQVRPVKITRHYTRYAEGSVLVEFGETKVLCNATVEETVPRFLKGQQQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTMEIQRLIARSLRAVVDLKALGERTVTVDCDVIQADGGTRTAAITGACVALHDAMSKLVADGVLKENPMKGLVA
AISVGIVDGNAVCDLEYVEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHMELLQLLDLAHQGINQLLDAQRKALAL
>Mature_238_residues
MRPNNRELNQVRPVKITRHYTRYAEGSVLVEFGETKVLCNATVEETVPRFLKGQQQGWVTAEYGMLPRSTHSRMQREAAK
GKQGGRTMEIQRLIARSLRAVVDLKALGERTVTVDCDVIQADGGTRTAAITGACVALHDAMSKLVADGVLKENPMKGLVA
AISVGIVDGNAVCDLEYVEDSNAETDMNVVMVEDGRLVEVQGTAEGEPFSHMELLQLLDLAHQGINQLLDAQRKALAL

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates [H]

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family [H]

Homologues:

Organism=Escherichia coli, GI157672248, Length=211, Percent_Identity=80.0947867298578, Blast_Score=358, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71981632, Length=187, Percent_Identity=28.8770053475936, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336 [H]

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C [H]

EC number: =2.7.7.56 [H]

Molecular weight: Translated: 25998; Mature: 25998

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPNNRELNQVRPVKITRHYTRYAEGSVLVEFGETKVLCNATVEETVPRFLKGQQQGWVT
CCCCCCCCCCCCCEEEHHHHHHHCCCCEEEEECCEEEEECCCHHHHHHHHHCCCCCCCEE
AEYGMLPRSTHSRMQREAAKGKQGGRTMEIQRLIARSLRAVVDLKALGERTVTVDCDVIQ
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTAAITGACVALHDAMSKLVADGVLKENPMKGLVAAISVGIVDGNAVCDLEYVED
CCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEECCCEEEEEEEECC
SNAETDMNVVMVEDGRLVEVQGTAEGEPFSHMELLQLLDLAHQGINQLLDAQRKALAL
CCCCCCCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPNNRELNQVRPVKITRHYTRYAEGSVLVEFGETKVLCNATVEETVPRFLKGQQQGWVT
CCCCCCCCCCCCCEEEHHHHHHHCCCCEEEEECCEEEEECCCHHHHHHHHHCCCCCCCEE
AEYGMLPRSTHSRMQREAAKGKQGGRTMEIQRLIARSLRAVVDLKALGERTVTVDCDVIQ
EECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEEE
ADGGTRTAAITGACVALHDAMSKLVADGVLKENPMKGLVAAISVGIVDGNAVCDLEYVED
CCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEEECCCEEEEEEEECC
SNAETDMNVVMVEDGRLVEVQGTAEGEPFSHMELLQLLDLAHQGINQLLDAQRKALAL
CCCCCCCEEEEEECCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA