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The map label for this gene is argG

Identifier: 126176306

GI number: 126176306

Start: 4830604

End: 4831842

Strand: Reverse

Name: argG

Synonym: Sbal_4121

Alternate gene names: 126176306

Gene position: 4831842-4830604 (Counterclockwise)

Preceding gene: 126176307

Following gene: 126176305

Centisome position: 94.24

GC content: 48.18

Gene sequence:

>1239_bases
ATGTCTATCGAGATAAAAAATACTGGCGTGAAAAAAGTGGTTTTAGCCTATTCAGGTGGTCTAGATACCTCGGCCATTAT
TCCTTGGTTAAAAGAAAACTACGATAACTGTGAAATCATCGCATTTTGCGCCGACGTCGGCCAAGGCGAAGAAGAGCTGG
TTGGCTTGACTGAAAAAGCCTTAGCTTCGGGTGCGTCTGAGTGTCATATTGTCGATCTGAAAGAAGAATTCGTTAAAGAG
TATATCTACCCAACGATTGCGAGCGGTGCGATTTACGAAGGCACTTATTTACTCGGTACGTCAATGGCGCGTCCTATCAT
TGCCAAAGCACAGGTTGAAGTGGCCCGTAAAGTGGGCGCCGATGCTCTGTGCCACGGTTGTACCGGTAAAGGTAACGATC
AAGTGCGTTTCGAAGGTTGTTTCGCTGCATTAGCGCCTGATTTAAAAGTGATTGCACCATGGCGTGAATGGACCATGCAG
AGCCGTGAAGATCTACTGGCCTATTTAGCCGAACGTGACATTAAGACGTCAGCGTCAGCGACTAAAATCTATAGCCGTGA
TGCCAATGCTTTCCATATTTCCCATGAAGGTGGTGAGTTAGAAGATCCATGGAACGAGCCAAGCAAAGGTGTGTGGACTC
TGACAGCCGATCCTGAAGATGCACCGAACAAGCCTGAATATGTGTCGCTAGAAGTAGAACATGGCCGTATCACTAAAGTG
AATGGTGAAGCCCTAACGCCTTATGCTGCGCTGATGACATTAAACGCGATTGCTGCGCCACACGGTGTGGGTCGTATCGA
CATCACGGAAAATCGTTTAGTGGGCATGAAGTCTCGTGGCTGTTACGAAACACCCGGCGGCACTGTGATGTTCGCAGCAC
TGCGTGCGATTGAAGAACTTGTACTCGACAAAACCAGCCGTAACTGGCGTGAGCAAGTTGCCGCACAAATGGCGCATTTA
GTGTATGACGGTCGTTGGTTCACGCCACTTTGTAAGTCACTGCTGGCAGCATCTGAGTCACTGGCAGAATCAGTTAACGG
TGAAGTAGTGGTTAAACTCTACAAAGGCCAAGCAACGGCAGTGAAGAAACGTTCACCTAATAGCTTGTACTCCGAAGCGT
TTGCCACCTTTGGTGAAGATCAAGTGTACGATCAAAAACATGCAGAAGGCTTTATCCGCCTGTACTCATTAGCGAGCCGC
ATCCGCGCCCTCAACGCTAATGACGTTAAGTCTAAGTAA

Upstream 100 bases:

>100_bases
GCATGCCCAAAATGCGGTACTGGTAACCTTATTTAGTTAAATTAACCCTGTTTAGCAGCCTGTTTTCTCCTAGGTGAGAA
ACGAGTCATAGGAATTTAAG

Downstream 100 bases:

>100_bases
TAGGTCGTTTAAAAGGGCGCCTAGCGCCCTTTTTTGTTTCTAGGTTTTGATCAAGTTTTTTGATTCAGATGAGTCGATTT
CAAATCAGCAGTGAGTAAGG

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKALASGASECHIVDLKEEFVKE
YIYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQ
SREDLLAYLAERDIKTSASATKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTADPEDAPNKPEYVSLEVEHGRITKV
NGEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSRNWREQVAAQMAHL
VYDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATAVKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASR
IRALNANDVKSK

Sequences:

>Translated_412_residues
MSIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKALASGASECHIVDLKEEFVKE
YIYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQ
SREDLLAYLAERDIKTSASATKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTADPEDAPNKPEYVSLEVEHGRITKV
NGEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSRNWREQVAAQMAHL
VYDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATAVKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASR
IRALNANDVKSK
>Mature_411_residues
SIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKALASGASECHIVDLKEEFVKEY
IYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGADALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQS
REDLLAYLAERDIKTSASATKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTADPEDAPNKPEYVSLEVEHGRITKVN
GEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEELVLDKTSRNWREQVAAQMAHLV
YDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATAVKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASRI
RALNANDVKSK

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=395, Percent_Identity=44.5569620253165, Blast_Score=308, Evalue=6e-84,
Organism=Homo sapiens, GI16950633, Length=395, Percent_Identity=44.5569620253165, Blast_Score=308, Evalue=6e-84,
Organism=Escherichia coli, GI1789563, Length=372, Percent_Identity=27.6881720430108, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6324514, Length=401, Percent_Identity=41.1471321695761, Blast_Score=294, Evalue=2e-80,
Organism=Drosophila melanogaster, GI21358151, Length=415, Percent_Identity=41.9277108433735, Blast_Score=305, Evalue=5e-83,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_SHEB2 (B8EBG0)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002359907.1
- ProteinModelPortal:   B8EBG0
- SMR:   B8EBG0
- GeneID:   7086227
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_4011
- HOGENOM:   HBG335267
- OMA:   VAVDVGQ
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 45080; Mature: 44948

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 44-44 BINDING 96-96 BINDING 101-101 BINDING 126-126 BINDING 128-128 BINDING 132-132 BINDING 132-132 BINDING 133-133 BINDING 136-136 BINDING 185-185 BINDING 194-194 BINDING 270-270 BINDING 282-282

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKA
CEEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHH
LASGASECHIVDLKEEFVKEYIYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGA
HHCCCCCEEEECHHHHHHHHHHCHHHCCCCEECCEEEECCHHCCHHHHHHHHHHHHHHCH
DALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQSREDLLAYLAERDIKTSASA
HHHHCCCCCCCCCCEEEEEEHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCH
TKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTADPEDAPNKPEYVSLEVEHGRITKV
HHHEECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEE
NGEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEEL
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHH
VLDKTSRNWREQVAAQMAHLVYDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATA
HHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEE
VKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASRIRALNANDVKSK
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SIEIKNTGVKKVVLAYSGGLDTSAIIPWLKENYDNCEIIAFCADVGQGEEELVGLTEKA
EEEEECCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHH
LASGASECHIVDLKEEFVKEYIYPTIASGAIYEGTYLLGTSMARPIIAKAQVEVARKVGA
HHCCCCCEEEECHHHHHHHHHHCHHHCCCCEECCEEEECCHHCCHHHHHHHHHHHHHHCH
DALCHGCTGKGNDQVRFEGCFAALAPDLKVIAPWREWTMQSREDLLAYLAERDIKTSASA
HHHHCCCCCCCCCCEEEEEEHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCH
TKIYSRDANAFHISHEGGELEDPWNEPSKGVWTLTADPEDAPNKPEYVSLEVEHGRITKV
HHHEECCCCEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEECCCEEEEE
NGEALTPYAALMTLNAIAAPHGVGRIDITENRLVGMKSRGCYETPGGTVMFAALRAIEEL
CCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCEECCCCCCCCCCCCCHHHHHHHHHHHHH
VLDKTSRNWREQVAAQMAHLVYDGRWFTPLCKSLLAASESLAESVNGEVVVKLYKGQATA
HHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEEE
VKKRSPNSLYSEAFATFGEDQVYDQKHAEGFIRLYSLASRIRALNANDVKSK
ECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA