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The map label for this gene is rep [H]

Identifier: 126176198

GI number: 126176198

Start: 4711711

End: 4713723

Strand: Reverse

Name: rep [H]

Synonym: Sbal_4012

Alternate gene names: 126176198

Gene position: 4713723-4711711 (Counterclockwise)

Preceding gene: 126176202

Following gene: 126176185

Centisome position: 91.93

GC content: 48.53

Gene sequence:

>2013_bases
ATGAAACTCAATCCCGCCCAAAACGATGCTGTTCATTATGTTTCAGGCCCTTGCCTAGTATTGGCGGGTGCGGGCAGTGG
TAAGACGCGCGTAATTATCAATAAAATCGGTTACTTGGTCGAAAAGTGCGGCTATAAGGCGCGCAATATTGCGGCGGTGA
CCTTCACCAACAAGGCTGCCCGTGAGATGAAAGAGCGGGTCGCTCAGTCCATGGGACGCAAAGAGGCCAGAGGTCTGTGG
ATTTCAACCTTCCACACCTTAGGGCTTGAAATCATTAAGCGTGAGCATAAGGTCTTAGGCTTAAAGCCGGGATTTTCGCT
GTTTGATGATCAAGATACCTTGGCACTGCTCAAAGAGTTAACCCAAGACGAGTTAGACGAAGATAAAGATTTACTCAAAG
CGCTCGCCAGTACGATTTCAAACTGGAAGGGCGGTTTAATTATTCCCGAGCATGCACGCAAGATCGCTAAAGATGAGCAG
CAGCATTTATTTGCCATGTTGTATCAGCGTTATGCCCAGCACATGAAAGCCTATAACGCCTTAGATTTTGACGATTTAAT
CCTGATGCCAACCTTATTGTTGCGGCATCATGAAGAAGTGCGTACCCGCTGGCAAACCAAGATCCAATACTTGCTGGTGG
ATGAATATCAAGATACCAATACCAGCCAATATGAGATGGTAAAGCTGTTAGTGGGTGATCGGGCGCGCTTTACCGTAGTG
GGTGACGACGATCAGTCCATCTATTCATGGCGCGGCGCTAAACCACAAAACTTAGTGTTATTGGGTACGGATTTTCCGCA
GCTTAAGTTAATTAAGCTCGAGCAGAATTACCGTTCTAGTCAACGGATCCTGCGGGCTGCTAACATTCTTATCGCGAACA
ATCCCCATGTTTACGATAAAGCGCTCTTTAGTGAGCTTGCCTATGGGGAGCCGCTGCGGGTGCTATTTGCCGCCAACGAG
GAGCAAGAGGCAGAACGTGTGGTCGCGGAAATTGTGCGACACAAGTTTGTTGGCCGTACTCAGTTTGGCGACTATGCGAT
TCTGTATCGCGGCAACCATCAATCGCGGTTACTCGAGCGCGCGCTGATGACGAACCGCATTCCTTACAAATTGAGTGGTG
GCACGTCATTCTTCGCCCGTGCTGAAATCAAGGACATCATGGGCTATCTAAAGCTGGTGGTGAATCCCGATGATGATAAT
GCTTTCCTGCGCATCGTTAACTTGCCTAAACGTGGCATAGGTCCATCGACTTTAGAGCGCTTAGGTAATTTCGCTAATGA
GAAGCATATTTCTATGTTTGCGGCGATTTTTGAGCCTGAACTCAATCATCATTTGCCGCCGGCGGCCATGTCATCCCTGT
ATCAATTCGGTCGTTTCATTGTCGAGACAGGGGATCATGCAGAGCGTGGTGAACCTGTCGAGGCTGTGAAGCATTTGATC
CGTAAGATCAATTATGAAGATTATCTATACGAAACCAGCACGAGTGCCAAAGCCGCTGAAATGCGGATGAAGAATATCTC
CGAGCTATACCGTTGGGTAACTGAGATGCTTGCAGGTGATGAATTAGATGAGCCAATGACCTTACCTGAGGTCGTGACTC
GACTGACGCTCAGAGACATGATGGAGCGCAATAGTGAGGACGAAGGCGGCGATCAAGTGCAGTTGATGACCTTGCATGCT
TCTAAGGGCTTAGAGTTCCCGTTTGTGTTTATGGTGGGAGTGGAGGAGCGGATCTTGCCCCATCAGACCAGCATAGATGA
AGACAATGTCGATGAAGAGCGCCGCTTAGCCTACGTGGGCATCACTCGGGCTCAACGTGAACTGTGGTTTACCCTCTGTC
GTGAAAGACGTCAATTTGGTGAAACCATGCGTTGTGAGCCGAGCCGTTTCTTGCTTGAGTTACCTCAAGATGATGTGGTG
TGGGAAAATCGTAAACCACAGCAGACAGAACAGGAGCGGATGCAGACTGGGCGAGCCAACATTTCTAGCTTAAGGGATAT
GTTTAAAAAATAA

Upstream 100 bases:

>100_bases
GAGTTCTGCTCAGCCCAAGCGGGGATTTGTTAGCCTTAAAACCGCCGATTCTGCTATGATCCCATCCCTTTCTGTCGACT
TGTTTGGATATTGCGCAAAG

Downstream 100 bases:

>100_bases
CGAGCGTTTCGCTTACTGTTATTTAAAGTCAATCGCTCGGGTTTTATCTAGGCATATTCGAGCTGAGTACAGATCTGTGA
TTGGGTGTCACTTAAGGTAA

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGYKARNIAAVTFTNKAAREMKERVAQSMGRKEARGLW
ISTFHTLGLEIIKREHKVLGLKPGFSLFDDQDTLALLKELTQDELDEDKDLLKALASTISNWKGGLIIPEHARKIAKDEQ
QHLFAMLYQRYAQHMKAYNALDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGDRARFTVV
GDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILRAANILIANNPHVYDKALFSELAYGEPLRVLFAANE
EQEAERVVAEIVRHKFVGRTQFGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDN
AFLRIVNLPKRGIGPSTLERLGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGDHAERGEPVEAVKHLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEPMTLPEVVTRLTLRDMMERNSEDEGGDQVQLMTLHA
SKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRCEPSRFLLELPQDDVV
WENRKPQQTEQERMQTGRANISSLRDMFKK

Sequences:

>Translated_670_residues
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGYKARNIAAVTFTNKAAREMKERVAQSMGRKEARGLW
ISTFHTLGLEIIKREHKVLGLKPGFSLFDDQDTLALLKELTQDELDEDKDLLKALASTISNWKGGLIIPEHARKIAKDEQ
QHLFAMLYQRYAQHMKAYNALDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGDRARFTVV
GDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILRAANILIANNPHVYDKALFSELAYGEPLRVLFAANE
EQEAERVVAEIVRHKFVGRTQFGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDN
AFLRIVNLPKRGIGPSTLERLGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGDHAERGEPVEAVKHLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEPMTLPEVVTRLTLRDMMERNSEDEGGDQVQLMTLHA
SKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRCEPSRFLLELPQDDVV
WENRKPQQTEQERMQTGRANISSLRDMFKK
>Mature_670_residues
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGYKARNIAAVTFTNKAAREMKERVAQSMGRKEARGLW
ISTFHTLGLEIIKREHKVLGLKPGFSLFDDQDTLALLKELTQDELDEDKDLLKALASTISNWKGGLIIPEHARKIAKDEQ
QHLFAMLYQRYAQHMKAYNALDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGDRARFTVV
GDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILRAANILIANNPHVYDKALFSELAYGEPLRVLFAANE
EQEAERVVAEIVRHKFVGRTQFGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDN
AFLRIVNLPKRGIGPSTLERLGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGDHAERGEPVEAVKHLI
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEPMTLPEVVTRLTLRDMMERNSEDEGGDQVQLMTLHA
SKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRCEPSRFLLELPQDDVV
WENRKPQQTEQERMQTGRANISSLRDMFKK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=668, Percent_Identity=67.5149700598802, Blast_Score=914, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=646, Percent_Identity=38.8544891640867, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=346, Percent_Identity=29.4797687861272, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=447, Percent_Identity=32.4384787472036, Blast_Score=196, Evalue=7e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 77151; Mature: 77151

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGYKARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEECCHHH
REMKERVAQSMGRKEARGLWISTFHTLGLEIIKREHKVLGLKPGFSLFDDQDTLALLKEL
HHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHH
TQDELDEDKDLLKALASTISNWKGGLIIPEHARKIAKDEQQHLFAMLYQRYAQHMKAYNA
HHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGDRARFTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILRAANILIANNPHVYDK
CCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCCHHHH
ALFSELAYGEPLRVLFAANEEQEAERVVAEIVRHKFVGRTQFGDYAILYRGNHQSRLLER
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHH
ALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDNAFLRIVNLPKRGIGPSTLER
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCCHHHHHH
LGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGDHAERGEPVEAVKHLI
HHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHH
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEPMTLPEVVTRLTLRDM
HHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNSEDEGGDQVQLMTLHASKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHEEEEE
ITRAQRELWFTLCRERRQFGETMRCEPSRFLLELPQDDVVWENRKPQQTEQERMQTGRAN
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHCCCC
ISSLRDMFKK
HHHHHHHHCC
>Mature Secondary Structure
MKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGYKARNIAAVTFTNKAA
CCCCCCCCCCEEEECCCEEEEEECCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEECCHHH
REMKERVAQSMGRKEARGLWISTFHTLGLEIIKREHKVLGLKPGFSLFDDQDTLALLKEL
HHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHH
TQDELDEDKDLLKALASTISNWKGGLIIPEHARKIAKDEQQHLFAMLYQRYAQHMKAYNA
HHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYEMVKLLVGDRARFTVV
CCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEE
GDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILRAANILIANNPHVYDK
CCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHHHHCEEEECCCCHHHH
ALFSELAYGEPLRVLFAANEEQEAERVVAEIVRHKFVGRTQFGDYAILYRGNHQSRLLER
HHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHH
ALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDNAFLRIVNLPKRGIGPSTLER
HHHHCCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCCHHHHHH
LGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGDHAERGEPVEAVKHLI
HHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHH
RKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEPMTLPEVVTRLTLRDM
HHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MERNSEDEGGDQVQLMTLHASKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVG
HHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCCCCCCCCHHHHEEEEE
ITRAQRELWFTLCRERRQFGETMRCEPSRFLLELPQDDVVWENRKPQQTEQERMQTGRAN
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCEECCCCCCHHHHHHHHHCCCC
ISSLRDMFKK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]