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The map label for this gene is fliA [H]

Identifier: 126176138

GI number: 126176138

Start: 4643718

End: 4644434

Strand: Reverse

Name: fliA [H]

Synonym: Sbal_3950

Alternate gene names: 126176138

Gene position: 4644434-4643718 (Counterclockwise)

Preceding gene: 126176139

Following gene: 126176137

Centisome position: 90.58

GC content: 47.0

Gene sequence:

>717_bases
ATGACAACAGCGGGCCAACTCGCATATCAACAAACCTGTGACTATCCGGTCGAAGAGCGCCAATCTGAACAGCAGATTAT
TCGGCAATATCTGCCATTAATTAAACGTATCGTGTCGCAGATGCGCAGTCACTGCGGCGTCATGTTATCCCTCGAGGATA
TGGAGCAGATTGGCATGATGGCCTTGCTTGAGTCATCACGTCGGTATCCTGGCGAGTTGGATAACGGATTTATTTCCTTC
GCGGGGCAACGTATTCGCGGTGCCATACTCGACGAGCTGAGGCGGCAAGATTGGCGACCTAGACCTGTTCGACAACAGGC
ACATGAGCTTAATGACGTGGTGAGACGCTTGAGTCGAGAGATGGGGCGTGAACCAAGCGATCTCGAAGTGGCCAGTGCGC
TTGGGCTGAGCGCAGAAGATTATCGGGAACGCTTATATTCAACACAAGCGGAGTCGATGAAGAGCTTAGACGAGTTGCTA
TTAGGAGGGGAGCAACCAGTAGACAGAAGCAATGATGTTGAACGATTGATGCTGAAGCAAACCTTGATCAAAGTTATTTC
AAAATTAAATGAAAGAGAACAGCTAATTCTGTCGCTTTACTATCATCATGAGCTGAATTTAAAAGAGATTGCTATGGCAT
TGGGATTGACTGAAACGCGTATTTGCCAGCTGCATAAATCAGCTATTTCACAGTTAAAAACAATATTGCAGCGCTAA

Upstream 100 bases:

>100_bases
CGCTGATATTGATGGTGAAAGTGCTGTCTGATAATAAATTACCGTCGGATATTGATGAAGTGCTCTTTACTCGCATGGTG
ATCCAGTAAGAGGCCCTATC

Downstream 100 bases:

>100_bases
AGTTTAAAAGTGAGAAGATCGTAATGAGTAAACTAGTTGGGCTGTTAATCATTTTAGCTTCTGTGTTTGGTGGGTATGTT
TGGGCGGGTGGGGCTTTGTC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-27; Sigma-F factor [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MTTAGQLAYQQTCDYPVEERQSEQQIIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMMALLESSRRYPGELDNGFISF
AGQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSREMGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELL
LGGEQPVDRSNDVERLMLKQTLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR

Sequences:

>Translated_238_residues
MTTAGQLAYQQTCDYPVEERQSEQQIIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMMALLESSRRYPGELDNGFISF
AGQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSREMGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELL
LGGEQPVDRSNDVERLMLKQTLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR
>Mature_237_residues
TTAGQLAYQQTCDYPVEERQSEQQIIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMMALLESSRRYPGELDNGFISFA
GQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSREMGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELLL
GGEQPVDRSNDVERLMLKQTLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=232, Percent_Identity=36.2068965517241, Blast_Score=144, Evalue=4e-36,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27542; Mature: 27411

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAGQLAYQQTCDYPVEERQSEQQIIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMM
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHH
ALLESSRRYPGELDNGFISFAGQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSRE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
MGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELLLGGEQPVDRSNDVERLMLKQ
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
TLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTAGQLAYQQTCDYPVEERQSEQQIIRQYLPLIKRIVSQMRSHCGVMLSLEDMEQIGMM
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHH
ALLESSRRYPGELDNGFISFAGQRIRGAILDELRRQDWRPRPVRQQAHELNDVVRRLSRE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
MGREPSDLEVASALGLSAEDYRERLYSTQAESMKSLDELLLGGEQPVDRSNDVERLMLKQ
HCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
TLIKVISKLNEREQLILSLYYHHELNLKEIAMALGLTETRICQLHKSAISQLKTILQR
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501040; 12620739 [H]