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The map label for this gene is tapD [H]

Identifier: 126176111

GI number: 126176111

Start: 4616576

End: 4617490

Strand: Direct

Name: tapD [H]

Synonym: Sbal_3921

Alternate gene names: 126176111

Gene position: 4616576-4617490 (Clockwise)

Preceding gene: 126176110

Following gene: 126176112

Centisome position: 90.04

GC content: 46.01

Gene sequence:

>915_bases
ATGTCAGAATTCATCTCCCTTTTGGGCCAGCACCCTTATCTCTTTATTGCTCTAAGCTTTATTTTTGCCGCTACCATTGG
TAGTTTTTTAAACGTGGTAATCCACAGATTTCCTGTGATGATGAAACGTGAGTGGCAGCAAGAATGTAATCAGTATTTGC
AGGAATACCACGAAGATATAGTCAACAAGATAGGTATAAACCAACTCAATAAACCTATTGATGCCTTCCCAGAGAAGTAT
AATCTGGTTGTGCCGGGCTCAGCCTGCCCTAAATGTAAAACGTCGATTAAGCCTTGGCATAATTTCCCCCTATTAGGCTG
GCTAATGCTCAAGGGAAAATGCGCCGCCTGTCAAGCAGCGATTTCAGCCCGCTACCCTATTATTGAATTCATCACAGGGC
TACTTATCGCGACTTTAGCTTGGCATTTTGGTCCAAGCTGGCAGTTTGTTTTTGCGAGCATACTCACCTTTGTGCTTGTA
GCACTGACAGCTATCGATCTTGATGAGATGCTGCTCCCCGACCAGATGACGTTGCCGTTACTTTGGCTAGGCTTGATAAT
CAACCTAAATCATACCTTTGCGAGTCCAACCGACGCCATTATGGGTGCGGCAGCAGGCTATTTAAGCTTGTGGTCTATTT
TTTGGCTATTTAAGATCCTCACCGGCAAGGAAGGCATGGGTTATGGTGACTTTAAACTGCTAGCCGTGTTCGGTGCTTGG
CTTGGCTGGCAAATGCTGCCGCTGGTGATTTTACTTTCGTCCCTCGTTGGCGCAGTCGTTGGCATCACTATGATTGTTAG
TAAACGCCTGAAACAAGGGAATCCAATCCCATTTGGCCCCTATATTGCCGCAGCTGGCTGGATAGCCTTGCTATTTGGGC
AACAGATTGTCGATTGGTACCTATCGACACTGTAA

Upstream 100 bases:

>100_bases
TGTCGTTGGTGGCCTAATTGTTGCTATGTATTTGCCCATCTTCCAAATGGGTAAAATCGTCAGCTAATCTATTTGATTCA
AATTAAAATAAGAAAATATA

Downstream 100 bases:

>100_bases
GATCTAGCAAATTAAGCACAAAGCTATCCGGAGTATGTTATGTCAAAGTTTGTCGTTGGCCTTACAGGCGGCATCGGCAG
CGGTAAAACGACTGTTGCCA

Product: prepilin peptidase

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSEFISLLGQHPYLFIALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDIVNKIGINQLNKPIDAFPEKY
NLVVPGSACPKCKTSIKPWHNFPLLGWLMLKGKCAACQAAISARYPIIEFITGLLIATLAWHFGPSWQFVFASILTFVLV
ALTAIDLDEMLLPDQMTLPLLWLGLIINLNHTFASPTDAIMGAAAGYLSLWSIFWLFKILTGKEGMGYGDFKLLAVFGAW
LGWQMLPLVILLSSLVGAVVGITMIVSKRLKQGNPIPFGPYIAAAGWIALLFGQQIVDWYLSTL

Sequences:

>Translated_304_residues
MSEFISLLGQHPYLFIALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDIVNKIGINQLNKPIDAFPEKY
NLVVPGSACPKCKTSIKPWHNFPLLGWLMLKGKCAACQAAISARYPIIEFITGLLIATLAWHFGPSWQFVFASILTFVLV
ALTAIDLDEMLLPDQMTLPLLWLGLIINLNHTFASPTDAIMGAAAGYLSLWSIFWLFKILTGKEGMGYGDFKLLAVFGAW
LGWQMLPLVILLSSLVGAVVGITMIVSKRLKQGNPIPFGPYIAAAGWIALLFGQQIVDWYLSTL
>Mature_303_residues
SEFISLLGQHPYLFIALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDIVNKIGINQLNKPIDAFPEKYN
LVVPGSACPKCKTSIKPWHNFPLLGWLMLKGKCAACQAAISARYPIIEFITGLLIATLAWHFGPSWQFVFASILTFVLVA
LTAIDLDEMLLPDQMTLPLLWLGLIINLNHTFASPTDAIMGAAAGYLSLWSIFWLFKILTGKEGMGYGDFKLLAVFGAWL
GWQMLPLVILLSSLVGAVVGITMIVSKRLKQGNPIPFGPYIAAAGWIALLFGQQIVDWYLSTL

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the tapA pilin precursor during membrane translocation [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=288, Percent_Identity=34.7222222222222, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1789732, Length=132, Percent_Identity=45.4545454545455, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 33960; Mature: 33829

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEFISLLGQHPYLFIALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDI
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNKIGINQLNKPIDAFPEKYNLVVPGSACPKCKTSIKPWHNFPLLGWLMLKGKCAACQAA
HHHHCHHHHCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
ISARYPIIEFITGLLIATLAWHFGPSWQFVFASILTFVLVALTAIDLDEMLLPDQMTLPL
HHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LWLGLIINLNHTFASPTDAIMGAAAGYLSLWSIFWLFKILTGKEGMGYGDFKLLAVFGAW
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LGWQMLPLVILLSSLVGAVVGITMIVSKRLKQGNPIPFGPYIAAAGWIALLFGQQIVDWY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSTL
HHHC
>Mature Secondary Structure 
SEFISLLGQHPYLFIALSFIFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHEDI
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNKIGINQLNKPIDAFPEKYNLVVPGSACPKCKTSIKPWHNFPLLGWLMLKGKCAACQAA
HHHHCHHHHCCCHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
ISARYPIIEFITGLLIATLAWHFGPSWQFVFASILTFVLVALTAIDLDEMLLPDQMTLPL
HHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LWLGLIINLNHTFASPTDAIMGAAAGYLSLWSIFWLFKILTGKEGMGYGDFKLLAVFGAW
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LGWQMLPLVILLSSLVGAVVGITMIVSKRLKQGNPIPFGPYIAAAGWIALLFGQQIVDWY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LSTL
HHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA