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The map label for this gene is tapB [H]

Identifier: 126176109

GI number: 126176109

Start: 4613428

End: 4615140

Strand: Direct

Name: tapB [H]

Synonym: Sbal_3919

Alternate gene names: 126176109

Gene position: 4613428-4615140 (Clockwise)

Preceding gene: 126176108

Following gene: 126176110

Centisome position: 89.98

GC content: 46.88

Gene sequence:

>1713_bases
ATGCCTACAACTGGTCTTCATTTAGGTTTATCCACCTTATTTATCCGTAAAGGTCTGCTAAATGAAGAGCAGATGGCTAA
CGCCATCACTAAATCCCGTCAAAACAAACACGCCCTTGTAAGCACTTTAGTTCAAAGTAAGCTGGTCTCTGCCCGTGCAA
TAGCAGATCTTTGCTACGAAGAATACGGCACGCCACTACTCGATCTCAATGAGTTTGATCTTGCTTCCATTCCTGAGGAT
TTTCTCAATAAAAAGCTGATTGAGAAACACCGCTGCTTACCCTTGTTTAAACGTGGGAATCGCCTCTATATAGGCACATC
CGACCCGACCAATATTGCCGCCCTTGAAGACTTCCAATTTAGTGCAGGCCTGCATGCCGAAGCGATTCTAGTGGAGGAAG
ATAAACTATCTAAAGCGCTGGAAAAAATTCTCGAGGAAGATATCAGTGGCCTAGATTTAGGCGGTATGGATGAAGCGGCG
CTGGCGGCCATAGAAATTACTGACACAGACAATCGCAGCGATGTGTCAGCAGGCGAGGCCAGTGACGACGCGCCTATTGT
TATTTATATAAACAAAATTCTAACCGACGCCATTCGCAAAGGCGCCTCGGATTTACACTTTGAGCCCTATGAAAAGCGCT
ACCGCATTCGTTTTCGCATCGACGGTATTCTCCACGAAGTCTCAGAGCCTCCTATTAGCCTCGCAGGCCGATTATCTGCT
AGGTTAAAGGTAATGTCTAAGCTCGATATCGCCGAACGGCGCGTGCCACAAGATGGTCGCATTAAAATGAAATTGTCCCG
TACTAAGTCCATCGACTTTCGGGTAAGTACCCTACCAACACTGTGGGGGGAAAAAATTGTAATGCGGATTTTGGACTCAT
CCTCCGCACAATTAGGCATTGAAAAGCTCGGCTACGAGCCCGACCAAGAAAAGCTCTACCTTGAAATGCTAGCAAAACCC
CAAGGTATGATTTTAGTGACTGGCCCTACGGGCTCAGGTAAAACCGTCTCCCTTTACACTGGGTTAAATATTCTCAATAC
CGAAGAGCGTAATATTTCCACCGCTGAAGATCCGGTTGAGATTAACCTTGAGGGCGTGAATCAGGTTCATATTAATTTAA
AAGCAGGGCTCACCTTCGCGTCCGCTTTACGTTCATTTCTACGCCAAGACCCCGATGTGGTTATGGTCGGGGAAATCCGT
GATCTTGAAACCGCCGAAATTGCTATTAAAGCGGCGCAAACGGGTCACTTAGTTTTATCAACCTTGCATACTAACTCGGC
AGCCGAAACCTTAACCCGTTTAATCAATATGGGTGTGCCTGGCTACAACATAGCCAGCTCTGTGAATCTGATTATTGCCC
AGCGCTTAGCCCGTCGTCTCTGCCCTGAATGCAAGCAAGCGGAAGTGATCCCCGAGCATGAATTATTGCGTTTAGGCTTT
GCCCAAGCACAAATTACTCAAGGCTTTACCACCTTTAAACCTATTGGCTGCGAGCACTGCTCTGCGGGCTACAAAGGCCG
AGTCGGTATTTATGAAGTGATGAAAATGTCCGATGAAATAGCCCGTACTATCATGGAGGGCGGTAATTCTTTGCAAATTG
CCTCCCAAGCTAAAGCACAAGGAATGCGAGATTTACGGCAATCAGGGTTATTGAAAGTGATGCATGGCGTCACTAGCATT
GCAGAAGTCAATCGCGTCACCAGTTTTGGCTAA

Upstream 100 bases:

>100_bases
TAACATACTAATTTATATATTCATTTATGACTATGTTGAGCACACTATAAATAAAACTAGTGAGCTCAACTTTTAGTTAT
TCATTTAAGTGACAATTCGA

Downstream 100 bases:

>100_bases
ATCTAGCATTTCTCATCTAAACTTAAAAAATATAGAGCGGTGCAAACTTCAATTTTAAAGCTAGCATAATTTAGCAGATG
CAGCTTTCTACTAATTGGCT

Product: type IV-A pilus assembly ATPase PilB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MPTTGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLVSARAIADLCYEEYGTPLLDLNEFDLASIPED
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKILEEDISGLDLGGMDEAA
LAAIEITDTDNRSDVSAGEASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
AQAQITQGFTTFKPIGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVMHGVTSI
AEVNRVTSFG

Sequences:

>Translated_570_residues
MPTTGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLVSARAIADLCYEEYGTPLLDLNEFDLASIPED
FLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKILEEDISGLDLGGMDEAA
LAAIEITDTDNRSDVSAGEASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
AQAQITQGFTTFKPIGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVMHGVTSI
AEVNRVTSFG
>Mature_569_residues
PTTGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLVSARAIADLCYEEYGTPLLDLNEFDLASIPEDF
LNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQFSAGLHAEAILVEEDKLSKALEKILEEDISGLDLGGMDEAAL
AAIEITDTDNRSDVSAGEASDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSAR
LKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGFA
QAQITQGFTTFKPIGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVMHGVTSIA
EVNRVTSFG

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=386, Percent_Identity=49.2227979274611, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1786296, Length=377, Percent_Identity=46.4190981432361, Blast_Score=346, Evalue=3e-96,
Organism=Escherichia coli, GI87082188, Length=281, Percent_Identity=33.4519572953737, Blast_Score=123, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62707; Mature: 62576

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLVSARAIADLCYE
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EYGTPLLDLNEFDLASIPEDFLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQF
HHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCEEEEECCCC
SAGLHAEAILVEEDKLSKALEKILEEDISGLDLGGMDEAALAAIEITDTDNRSDVSAGEA
CCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCC
SDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
CCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCCHHHHHHHHH
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGI
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCHHCCH
EKLGYEPDQEKLYLEMLAKPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVE
HHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEECCCCCCCCCCCCCEE
INLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHLVLS
EEEECCEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCCEEEE
TLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
EECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AQAQITQGFTTFKPIGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQ
HHHHHHCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
GMRDLRQSGLLKVMHGVTSIAEVNRVTSFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTTGLHLGLSTLFIRKGLLNEEQMANAITKSRQNKHALVSTLVQSKLVSARAIADLCYE
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EYGTPLLDLNEFDLASIPEDFLNKKLIEKHRCLPLFKRGNRLYIGTSDPTNIAALEDFQF
HHCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHCCCEEEEECCCCCCEEEEECCCC
SAGLHAEAILVEEDKLSKALEKILEEDISGLDLGGMDEAALAAIEITDTDNRSDVSAGEA
CCCCCEEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHEEEEEEECCCCCCCCCCCCC
SDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
CCCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHHHCCCCCHHHHHHHHH
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGI
HHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCHHCCH
EKLGYEPDQEKLYLEMLAKPQGMILVTGPTGSGKTVSLYTGLNILNTEERNISTAEDPVE
HHCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCEEECCCCCCCCCCCCCEE
INLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIRDLETAEIAIKAAQTGHLVLS
EEEECCEEEEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCCEEEE
TLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
EECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AQAQITQGFTTFKPIGCEHCSAGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQ
HHHHHHCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
GMRDLRQSGLLKVMHGVTSIAEVNRVTSFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]