Click here to switch to the map view.

The map label for this gene is acnB [H]

Identifier: 126176095

GI number: 126176095

Start: 4588064

End: 4590736

Strand: Reverse

Name: acnB [H]

Synonym: Sbal_3905

Alternate gene names: 126176095

Gene position: 4590736-4588064 (Counterclockwise)

Preceding gene: 126176098

Following gene: 126176082

Centisome position: 89.53

GC content: 48.26

Gene sequence:

>2673_bases
ATGTCGGACTGTCAGCTTCAATTAAGAAGACGTGCAGTTTCGTTGTTAGAGCGCCAAACAAAGAGGAATGTTGCCGTGCT
AGAAGCATATCGTAAACACGTCGCAGAACGTGCTGCAGAGGGCGTAGTCCCTAAGCCATTAGATGCACATCAAGTGGCTG
AACTGGTTAAGTTAGTTCAAAACCCACCCGCAGGTGAAGAGGCTTTCGTTCTCGACCTGCTTGAAAATCGAATTCCCCCA
GGTGTTGATGAAGCCGCTTATGTTAAGGCTGCTTTCTTAGATGCGGTTGCCAAAGGCACTGAAAAATCACCTATTTTGTC
TGCTGAGCGTGCTACTGAGTTATTAGGCACTATGCAAGGCGGCTACAACATCGAACCTCTGATCGCGCAGCTGGATAACC
CAGCGTTAGCACCTTTAGCCGTTAAAGCCTTATCACACACTCTGCTGATGTTTGATTCTTTCCACGATGTGGTTGAGAAG
ATGGAAGCAGGCAATGAGTTTGCTAAGCAAGTGGTTGAAGCGTGGGCGAATGCAGATTGGTACTTAAACCGTCCAAAACT
GGCCGACAAAGTCACTCTGACAGTATTTAAAGTCTCTGGTGAAACCAACACCGATGACTTGTCACCAGCACCCGATGCTT
GGTCACGTCCTGATATTCCATTACACGCGTTAGCTATGCTGAAAAACGCCCGTGATGGTATCGAGCCTGACGTTGCTGGC
GCCGTTGGCCCAGTGAAAAAAATCGAAGCGTTAAAAGCGCAAGGTTTCCCACTAGTCTATGTGGGCGATGTTGTGGGTAC
GGGTTCATCACGTAAATCAGCGACTAACTCAGTACTGTGGTTTATGGGCGATGACATTCCATTCGTACCCAACAAGCGCG
CTGGCGGTTTCTGCCTTGGCGGTAAAATTGCACCGATTTTCTTCAACACTATGGAAGATGCGGGCGCACTGCCAATCGAG
CTTGACGTAAACAAAATGGAAATGGGCGATGTGATTGACATCTACCCATACGCCGGTGTGGTTAAGCGTCACGACAGCGA
CGAAGTGATTTCAGAATTCAGCTTAAAAACTGAAGTGCTGTTAGACGAAGTTCGTGCTGGTGGTCGTATTCCACTGATCA
TCGGTCGTGGTTTAACTGACAAAGCCCGTGAAGTATTAGGTTTAGGCGTATCTGACGTATTCGTACGTTCACAAGACGTG
GCTGATACGGGTAAAGGTTATACGCTTGCACAGAAAATGGTTGGTAAAGCATGCGGCGTAGCTGGCGTGCGTCCTGGCCA
ATACTGCGAACCTAAGATGACCTCTGTTGGTTCACAAGATACCACAGGTCCTATGACCCGTGACGAGCTGAAAGATTTAG
CTTGCTTAGGTTTCAGTGCTGATTTGACTATGCAGTCATTCTGTCACACTGCGGCTTATCCAAAGCCTGTTGATGTGAAC
ACACATCATACTCTGCCAGATTTCATCATGAACCGCGGCGGTGTATCACTGCGTCCAGGCGATGGCGTTATCCACTCATG
GTTAAACCGTATGCTGTTGCCAGATACCGTTGGTACGGGTGGTGACTCACATACGCGTTTCCCAATCGGTATTTCATTCC
CAGCAGGTTCTGGCTTAGTGGCGTTTGCTGCGGCGACTGGCGTTATGCCGCTCGACATGCCTGAATCAGTATTAGTGCGC
TTCAAGGGCAAAATGCAACCCGGTATCACACTGCGTGATCTGGTACATGCTATCCCATTAAAAGCCATTGAACTGGGCAT
GCTGACCGTTGAGAAGAAAGGTAAAGTCAACATCTTCTCTGGTCGCGTACTGGAAATCGAAGGTCTTGAAACCTTGAAAG
TTGAACAAGCTTTCGAACTATCTGATGCTTCAGCTGAGCGCTCTGCTGCCGGTTGTACCATCAAGTTAGACAAAGAACCT
ATCATCGAATACCTGAACTCAAACATTATCATGCTGAAGTGGATGATCGCTGAAGGTTACGGCGATCGTCGTACGATTGA
ACGTCGTATCAAAGGCATGGAAGAGTGGTTAGCGAATCCTGAACTGATGAGCGCCGATAAAGATGCTGAGTACGCTGCAG
TTATCGAAATCGATTTAAACGACATCAAAGAGCCAATCCTGTGTGCGCCGAACGATCCAGATGATGCAGTACTACTGTCA
TCTGTTGCGCAAACTAAGATTGACGAAGTGTTCGTCGGTTCTTGTATGACCAACATTGGTCACTTCCGCGCAACCGGTAA
GATGTTAGATAAGTTTGCTAAAACCCTGCCAACTCGTCTGTGGATTGCACCACCGACTAAGATGGACAGAGATCAGCTGA
CTGAAGAAGGCTACTACGCGATTTTCGGTCGTGTAGGCGCGCGCATCGAGATCCCAGGCTGTTCACTGTGTATGGGTAAC
CAAGCACGTGTAGCTGAAGGTGCAACTGTCGTATCGACTTCTACCCGTAACTTCCCTAACCGTTTAGGTACAGGCGCTAA
CGTTTACTTAGCCTCTGCTGAACTGGCTGCGGTTGCTGCACTGTTAGGTCGTCTGCCGTCACCTGAAGAATATCAAGAAT
ACGCAAAAGAGTTAGACGCCACAGCGGCTGACACTTACCGTTACTTGAACTTCGATCAAATCGAGTCTTATACACAAAAG
GCATCGGAAGTGATTTTCCAGACTACCGTGTAA

Upstream 100 bases:

>100_bases
GCCGCCGGATTTAGTCCTTATTTCGCTTTTTGCCGAGTGTGACCTGATTCATACTTTTGCAAAGCTGTAGTATACTGCAG
GCCGGAAATACGGGTCGATC

Downstream 100 bases:

>100_bases
TCGATTGCCGTTAAGGTAGCTTGATGTACAAAAGCCAGCGCAAGCTGGCTTTTTTGTAGGTCAGTTTCTCCTCCACATAT
ATATGCACAATTAACCCCTC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 890; Mature: 889

Protein sequence:

>890_residues
MSDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAFVLDLLENRIPP
GVDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEK
MEAGNEFAKQVVEAWANADWYLNRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAG
AVGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIE
LDVNKMEMGDVIDIYPYAGVVKRHDSDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDV
ADTGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVR
FKGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEP
IIEYLNSNIIMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLS
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGN
QARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQK
ASEVIFQTTV

Sequences:

>Translated_890_residues
MSDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAFVLDLLENRIPP
GVDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEK
MEAGNEFAKQVVEAWANADWYLNRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAG
AVGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIE
LDVNKMEMGDVIDIYPYAGVVKRHDSDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDV
ADTGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVR
FKGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEP
IIEYLNSNIIMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLS
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGN
QARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQK
ASEVIFQTTV
>Mature_889_residues
SDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAFVLDLLENRIPPG
VDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEKM
EAGNEFAKQVVEAWANADWYLNRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAGA
VGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIEL
DVNKMEMGDVIDIYPYAGVVKRHDSDEVISEFSLKTEVLLDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDVA
DTGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNT
HHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRF
KGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFSGRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEPI
IEYLNSNIIMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLSS
VAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYAIFGRVGARIEIPGCSLCMGNQ
ARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQKA
SEVIFQTTV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=865, Percent_Identity=81.1560693641619, Blast_Score=1447, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=386, Percent_Identity=25.3886010362694, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=26.509186351706, Blast_Score=84, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 96574; Mature: 96443

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQ
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NPPAGEEAFVLDLLENRIPPGVDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQG
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
GYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEKMEAGNEFAKQVVEAWANADW
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
YLNRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAG
EECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
AVGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLG
CCCHHHHHHHHHCCCCCEEEECCEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEC
GKIAPIFFNTMEDAGALPIELDVNKMEMGDVIDIYPYAGVVKRHDSDEVISEFSLKTEVL
CEEEEEEEECHHCCCCCEEEEECCCCCCCCEEEECCCCCHHHCCCCHHHHHHHHHHHHHH
LDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDVADTGKGYTLAQKMVGKACGV
HHHHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
AGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
CCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLV
CCCCCHHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCE
AFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFS
EEHHHCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHCCEEEEECCCCEEEEC
GRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIIMLKWMIAEGY
CEEEEECCCCEEEHHHHHCCCCCCCHHCCCCCEEEECHHHHHHHHCCCEEEEEEEEECCC
GDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHH
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEE
IFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAA
EEECCCCEEECCCCCEECCCCCCCCCCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHH
LLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQKASEVIFQTTV
HHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHEECC
>Mature Secondary Structure 
SDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQ
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NPPAGEEAFVLDLLENRIPPGVDEAAYVKAAFLDAVAKGTEKSPILSAERATELLGTMQG
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
GYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEKMEAGNEFAKQVVEAWANADW
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCE
YLNRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVAG
EECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
AVGPVKKIEALKAQGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLG
CCCHHHHHHHHHCCCCCEEEECCEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEC
GKIAPIFFNTMEDAGALPIELDVNKMEMGDVIDIYPYAGVVKRHDSDEVISEFSLKTEVL
CEEEEEEEECHHCCCCCEEEEECCCCCCCCEEEECCCCCHHHCCCCHHHHHHHHHHHHHH
LDEVRAGGRIPLIIGRGLTDKAREVLGLGVSDVFVRSQDVADTGKGYTLAQKMVGKACGV
HHHHHCCCCEEEEEECCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
AGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
CCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLV
CCCCCHHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCE
AFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLKAIELGMLTVEKKGKVNIFS
EEHHHCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHCCHHHHHCCEEEEECCCCEEEEC
GRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNIIMLKWMIAEGY
CEEEEECCCCEEEHHHHHCCCCCCCHHCCCCCEEEECHHHHHHHHCCCEEEEEEEEECCC
GDRRTIERRIKGMEEWLANPELMSADKDAEYAAVIEIDLNDIKEPILCAPNDPDDAVLLS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHH
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDRDQLTEEGYYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCEE
IFGRVGARIEIPGCSLCMGNQARVAEGATVVSTSTRNFPNRLGTGANVYLASAELAAVAA
EEECCCCEEECCCCCEECCCCCCCCCCCEEEECCCCCCHHHHCCCCEEEEEHHHHHHHHH
LLGRLPSPEEYQEYAKELDATAADTYRYLNFDQIESYTQKASEVIFQTTV
HHHCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]