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The map label for this gene is hslU

Identifier: 126176059

GI number: 126176059

Start: 4542264

End: 4543592

Strand: Direct

Name: hslU

Synonym: Sbal_3868

Alternate gene names: 126176059

Gene position: 4542264-4543592 (Clockwise)

Preceding gene: 126176058

Following gene: 126176060

Centisome position: 88.59

GC content: 49.81

Gene sequence:

>1329_bases
ATGTCTGAAATGACCCCCCGTGAGATTGTCCACGAGCTGGATGCACACATTATTGGCCAACAAAAGGCCAAACGTTCTGT
TGCTGTTGCCCTGCGTAACCGCTGGCGCCGGATGCAACTGGATGTGGATTTCCGTCAAGAAGTCACCCCAAAAAACATCC
TGATGATTGGCCCAACTGGCGTAGGTAAAACTGAAATTGCCCGCCGTTTAGCTAAGCTAGCCAACGCGCCTTTTATCAAG
GTCGAAGCCACTAAATACACAGAAGTCGGCTATGTCGGTAAAGAAGTTGAGCAAATCATTCGCGACTTAACCGATATCGC
CATCAAGCTGACCCGTGAACAGCAAATGGGTAAGTGCCGCCAACGCGCCGAAGAAAATGCCGAAGAGCGCATTCTCGATG
CGCTGTTGCCTAAGCCGAAAAATGACTGGGAAAGCACAGAAACCGATTCCAGCTCAAACACCCGCCAAGTGTTCCGCAAG
AAGCTGCGCGAAGGCCAATTAGACGATAAAGAAATCGATATCGATGTGGCACAACCACAGGTCGGCGTCGAAATCATGTC
GCCTCCTGGCATGGAAGAAATGACCAACCAGCTGCAAAGCCTGTTTAAAAACATGGGCCAAGCACCGGCAAAACGTCGCA
AGATGAAGATCAAAGAAGCCTTTAAGCTGTTGATCGAAGAAGAAGCCGCCAAACTGGTCAATCAAGAAGATTTAAAAGAG
CAAGCCATTGAAATGGTTGAGCAACACGGCATAGTGTTCCTCGACGAAATCGACAAAATCTGTAAGCGTGGCGAAACCTC
AGGCCCAGACGTTTCCCGTGAAGGCGTGCAACGTGACTTACTGCCGCTGATCGAAGGCTGCACAGTCACCACAAAACACG
GCATGGTTAAAACCGACCACATTCTGTTTATCGCTTCGGGCGCATTCCAGATGTCGAAACCATCGGATTTAATTCCTGAG
TTACAGGGTCGTTTGCCGATCCGTGTCGAGTTAGATGCACTATCAGCCAATGATTTTAAACGCATTTTAACCGAGCCACA
CGCCTCGCTCACCGAGCAATATATCGCGCTGATGAACACAGAAGGCGTGAAAGTTGAATTCAGCGAATCGGGCATCGACA
GCATTGCAAAAGCCGCATGGCAAGTGAACGAGCGCACCGAAAACATCGGCGCCCGTCGTTTACACACTGTGATGGAAAAG
CTGATGGAAGATATCTCCTACGAGGCATCGGAAAAATCGGGCTCCGCCTTTGTTATCGACGCCGACTATGTCAGCGCCCA
CTTAGATAACTTAGTCCAAGACGAAGATCTCAGCCGCTTTATCCTCTAG

Upstream 100 bases:

>100_bases
GCTGAAAAATCCCTGACCATTGCCGCGGATATCTGCGTATTCACCAATCAATTCAAGACGATTGAAGAACTCAATTACTA
AACAGCCCGTGAGGAATATT

Downstream 100 bases:

>100_bases
TGCAAAACCCGTTCACCGCCAAACCCAAACGACTTGGCGGTGAACGGCGACTCAAATGCAAAAGTGTGACTCAACCGAAA
AGTCGACTCCAAACGCTAGG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDVDFRQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKYTEVGYVGKEVEQIIRDLTDIAIKLTREQQMGKCRQRAEENAEERILDALLPKPKNDWESTETDSSSNTRQVFRK
KLREGQLDDKEIDIDVAQPQVGVEIMSPPGMEEMTNQLQSLFKNMGQAPAKRRKMKIKEAFKLLIEEEAAKLVNQEDLKE
QAIEMVEQHGIVFLDEIDKICKRGETSGPDVSREGVQRDLLPLIEGCTVTTKHGMVKTDHILFIASGAFQMSKPSDLIPE
LQGRLPIRVELDALSANDFKRILTEPHASLTEQYIALMNTEGVKVEFSESGIDSIAKAAWQVNERTENIGARRLHTVMEK
LMEDISYEASEKSGSAFVIDADYVSAHLDNLVQDEDLSRFIL

Sequences:

>Translated_442_residues
MSEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDVDFRQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKYTEVGYVGKEVEQIIRDLTDIAIKLTREQQMGKCRQRAEENAEERILDALLPKPKNDWESTETDSSSNTRQVFRK
KLREGQLDDKEIDIDVAQPQVGVEIMSPPGMEEMTNQLQSLFKNMGQAPAKRRKMKIKEAFKLLIEEEAAKLVNQEDLKE
QAIEMVEQHGIVFLDEIDKICKRGETSGPDVSREGVQRDLLPLIEGCTVTTKHGMVKTDHILFIASGAFQMSKPSDLIPE
LQGRLPIRVELDALSANDFKRILTEPHASLTEQYIALMNTEGVKVEFSESGIDSIAKAAWQVNERTENIGARRLHTVMEK
LMEDISYEASEKSGSAFVIDADYVSAHLDNLVQDEDLSRFIL
>Mature_441_residues
SEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDVDFRQEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKYTEVGYVGKEVEQIIRDLTDIAIKLTREQQMGKCRQRAEENAEERILDALLPKPKNDWESTETDSSSNTRQVFRKK
LREGQLDDKEIDIDVAQPQVGVEIMSPPGMEEMTNQLQSLFKNMGQAPAKRRKMKIKEAFKLLIEEEAAKLVNQEDLKEQ
AIEMVEQHGIVFLDEIDKICKRGETSGPDVSREGVQRDLLPLIEGCTVTTKHGMVKTDHILFIASGAFQMSKPSDLIPEL
QGRLPIRVELDALSANDFKRILTEPHASLTEQYIALMNTEGVKVEFSESGIDSIAKAAWQVNERTENIGARRLHTVMEKL
MEDISYEASEKSGSAFVIDADYVSAHLDNLVQDEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=443, Percent_Identity=77.2009029345372, Blast_Score=699, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=215, Percent_Identity=34.8837209302326, Blast_Score=97, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_SHEB2 (B8E6E3)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002356429.1
- ProteinModelPortal:   B8E6E3
- SMR:   B8E6E3
- GeneID:   7086948
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_0472
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 50007; Mature: 49876

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 255-255 BINDING 320-320 BINDING 392-392

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDVDFRQEVTPKNILMIGPTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKYTEVGYVGKEVEQIIRDLTDIAIKLTREQQMGKCR
CCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRAEENAEERILDALLPKPKNDWESTETDSSSNTRQVFRKKLREGQLDDKEIDIDVAQPQ
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
VGVEIMSPPGMEEMTNQLQSLFKNMGQAPAKRRKMKIKEAFKLLIEEEAAKLVNQEDLKE
CCEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
QAIEMVEQHGIVFLDEIDKICKRGETSGPDVSREGVQRDLLPLIEGCTVTTKHGMVKTDH
HHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCEEECC
ILFIASGAFQMSKPSDLIPELQGRLPIRVELDALSANDFKRILTEPHASLTEQYIALMNT
EEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCC
EGVKVEFSESGIDSIAKAAWQVNERTENIGARRLHTVMEKLMEDISYEASEKSGSAFVID
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
ADYVSAHLDNLVQDEDLSRFIL
HHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SEMTPREIVHELDAHIIGQQKAKRSVAVALRNRWRRMQLDVDFRQEVTPKNILMIGPTG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKYTEVGYVGKEVEQIIRDLTDIAIKLTREQQMGKCR
CCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRAEENAEERILDALLPKPKNDWESTETDSSSNTRQVFRKKLREGQLDDKEIDIDVAQPQ
HHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCC
VGVEIMSPPGMEEMTNQLQSLFKNMGQAPAKRRKMKIKEAFKLLIEEEAAKLVNQEDLKE
CCEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
QAIEMVEQHGIVFLDEIDKICKRGETSGPDVSREGVQRDLLPLIEGCTVTTKHGMVKTDH
HHHHHHHHCCCEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCCEEECC
ILFIASGAFQMSKPSDLIPELQGRLPIRVELDALSANDFKRILTEPHASLTEQYIALMNT
EEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHCC
EGVKVEFSESGIDSIAKAAWQVNERTENIGARRLHTVMEKLMEDISYEASEKSGSAFVID
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
ADYVSAHLDNLVQDEDLSRFIL
HHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA