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The map label for this gene is fixJ [H]

Identifier: 126176053

GI number: 126176053

Start: 4537540

End: 4538142

Strand: Direct

Name: fixJ [H]

Synonym: Sbal_3862

Alternate gene names: 126176053

Gene position: 4537540-4538142 (Clockwise)

Preceding gene: 126176051

Following gene: 126176054

Centisome position: 88.5

GC content: 47.26

Gene sequence:

>603_bases
ATGTCTACAAAATTGCCACTGTATTTAGTTGATGACGATGAAGCGATTCTGGATTCGCTGGGTTTTATGCTTGGGCAATT
TGGCTACCAAGTGCAAACCTTCAATAGCGGCCGCGACTTCCTTGCGCACTGTGCATTATCGCAGGCAGGCTGCGTGATTT
TAGATAGCCGAATGCCAGAAATCACCGGCCAAGAAGTGCAGCAAAAACTGATCGAAACCCACAGCCCGCTAGGCGTCATC
TTTCTCACTGGCCACGGCGATTTACCTATGGCGCTGAGCGCCTTTCGTAAGGGAGCCTGCGACTTTTTTCAAAAACCTGT
GTCCGGCAAGGCATTAGTGCAAGCGATAGAAAAGGCGCAACGTGAAAGCCAAACGACCTTCGAGCAGCAAAGTTTACAAC
ATAAGTTTGACCAACTGACCGAGCGCGAGCAGCAAGTGTTAGCCCATGTGATCCAAGGCATGACCAATAAGCAAATCTCG
GAGGCGATGTTTTTATCCCTACGCACGATTGAAGTACACCGAGCAAAAATCATGAAAAAACTCGAAGTGAATAATATGGC
AGAGTTAGTGCAGCACTTAGGACATTTGCATTCTGTTTTATAA

Upstream 100 bases:

>100_bases
CTAATTTAGTGATGAACCTCCCAAGACCTAGGCTTAAAATTGAGCCTGACACAGACGCGGCTTAAGCTGATATAGCTCTC
CCGCCTCCATAAGGAAATCC

Downstream 100 bases:

>100_bases
ATGCTGAACCAATATTAAGTGCGCAATAAAAGATCGCCCTAATAAGGGAATAAACTCTAATTAAAAACCATTCCGCGACG
CTATTTAGATGATGGTTAAA

Product: response regulator receiver protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MSTKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPEITGQEVQQKLIETHSPLGVI
FLTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQRESQTTFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQIS
EAMFLSLRTIEVHRAKIMKKLEVNNMAELVQHLGHLHSVL

Sequences:

>Translated_200_residues
MSTKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPEITGQEVQQKLIETHSPLGVI
FLTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQRESQTTFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQIS
EAMFLSLRTIEVHRAKIMKKLEVNNMAELVQHLGHLHSVL
>Mature_199_residues
STKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPEITGQEVQQKLIETHSPLGVIF
LTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQRESQTTFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQISE
AMFLSLRTIEVHRAKIMKKLEVNNMAELVQHLGHLHSVL

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=205, Percent_Identity=26.8292682926829, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1790102, Length=166, Percent_Identity=31.3253012048193, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790299, Length=197, Percent_Identity=27.9187817258883, Blast_Score=68, Evalue=4e-13,
Organism=Escherichia coli, GI1788905, Length=124, Percent_Identity=36.2903225806452, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790860, Length=115, Percent_Identity=31.304347826087, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22408; Mature: 22277

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPE
CCCCCCEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCEEEECCCCCC
ITGQEVQQKLIETHSPLGVIFLTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQ
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RESQTTFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQISEAMFLSLRTIEVHRAKIMKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEVNNMAELVQHLGHLHSVL
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STKLPLYLVDDDEAILDSLGFMLGQFGYQVQTFNSGRDFLAHCALSQAGCVILDSRMPE
CCCCCEEEECCCHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHCCEEEECCCCCC
ITGQEVQQKLIETHSPLGVIFLTGHGDLPMALSAFRKGACDFFQKPVSGKALVQAIEKAQ
CCHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
RESQTTFEQQSLQHKFDQLTEREQQVLAHVIQGMTNKQISEAMFLSLRTIEVHRAKIMKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEVNNMAELVQHLGHLHSVL
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275 [H]