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The map label for this gene is epsE [H]

Identifier: 126176023

GI number: 126176023

Start: 4503300

End: 4505060

Strand: Reverse

Name: epsE [H]

Synonym: Sbal_3832

Alternate gene names: 126176023

Gene position: 4505060-4503300 (Counterclockwise)

Preceding gene: 126176024

Following gene: 126176022

Centisome position: 87.86

GC content: 49.18

Gene sequence:

>1761_bases
ATGAAACCCAGATTAAAAATGCGTTTGGGCGATCTGTTAGTTCAAGAAGCCATTATTACCGAAGCGCAGTTGCAACAGGC
GTTAGGCGAGCAGCGTAATACGGGCAAAAAACTGGGTCGTACCCTCATTGATCTGCAATCGATTACTGAGCATCAGTTGT
TGCAGTTCTTGTCGCAGCAATTGAATGTGCCTTTGCTTGATATCAGTAAAATGCCGATCCCAGCGGAAGTCGTGCATCTT
ATTCCTGAGGTGCAAGCCCGACGTTTCCGCGCTTTAGCGGTTGAAGACAGGGGCGATAGCGTACTGGTCGCTATGAGTGA
TCCTACCGATTTGCAGGCGCTCGATCACCTCGAAATGCTTATCGCACCTAAGCGTTTGAAGTTAGCGGTAGCGCCCGAGC
AGCAACTGCTGCAAGCGTTTGATAACTTATATCGCCGTACAGACCAAATTGCTCAGATCGCCGGTAAGCTCGAAGAAGAA
TACGCCGCCGATGAGATGTTCGATTTAGCCAGTCTGACTTCGGGTGACAGTGACAATGAAACCACAGTGGTTAAGTTGCT
ACAGTCGATTTTTGAGGATGCGGTGCAAATGCGCGCCTCGGATATCCATATCGAACCCGGTGATAAAGCCCTACGTATTC
GCCAGCGTATCGATGGCCAGCTACACGAAACGATTCTGAATGAAGTTAATATTGCCGCGGCTTTAGTATTGCGACTTAAG
TTGATGGCTGGACTCGATATTTCTGAAAAACGTTTGCCGCAGGATGGCCGTTTCCACATGGAAATCAAAGGTCACCATAT
CGATGTGCGTATGTCGACTATGCCGATTTACCACGGTGAATCTGTGGTAATGCGTTTACTCGACCAATCGGCGGGGTTAT
TGACCCTAAACGAAACCGGTATGCCGCCTGCTATTTTGGCGCGTATTCGTAAACAGATTAAACGCCCCCACGGCATGTTG
TTGGTCACTGGTCCCACGGGTAGCGGTAAAACCACCACCTTGTACGGTATTTTGAGCGAGCTCAATACCGCCGACCGTAA
GATCATCACAGTGGAAGATCCGGTCGAGTATCAATTGCCACGGATCAACCAAGTACAAGTTAACCATAAGATTGGCTTAA
ACTTTTCCAACGTATTGCGAACTACCTTACGTCAAGACCCCGATATCATCATGGTAGGGGAAATGCGTGACCAAGAAACC
GTTGAGATTGGCCTGAGGGGCGCATTAACGGGTCACTTTGTGCTATCGACCTTGCACACTAACGATGCGGTCACCAGTGC
GCTGCGTTTACTCGATATGGGCGCTGCGAGTTATTTAGTTGCCAGTGCCTTAAGGGTGATTATTGCCCAGCGTTTAGTGC
GCCGCGTGTGCCATAACTGTGGTGTTGAGTATCAGCCGACATCGCAGGAAAAAGCCTGGCTGAGTAGCGTGAGTCGTAAA
GATTTTACCCATGCAAACTTTAGAATCGGTACCGGTTGCCAGAGTTGTAACGGCAGTGGTTATCGTGGTCGTATTGGTAT
TTTTGAGATCCTCGAGCTCGACGAAAAGATGATCGATGCGATGCGTACCGGTAATCCGCAGGATTTTGCCAGAGCCGCGT
TGCAGAGCCCGAATTTTACTCCGCTCGCCGAGTCAGCGTTGCAATATTTGAGCGAAGGCATGACGACCATTGAAGAGGTG
GCTAAGCTGGTAGAGGATGTGAGTGAATCACAGATACCTTTGTCGAAGGATATTATTAGCCAACAAGGCGTTGAGGCGTA
A

Upstream 100 bases:

>100_bases
AAGCACGCCAAGCCTATCGGACTGCGTTAGGCCACAGAGGCTTATCGGCGCAGGCGAGTGCCTTTATCGAACAACGTTTG
ACTCAGCTAGGAGATAGCCA

Downstream 100 bases:

>100_bases
TTATGCCGATTTATCAATACCGTGGACGCAGTGGTCAAGGTCAATCCGTTACAGGGCAATTGGATGCGGCATCGGAAAGT
GCCGCTGCTGACATGCTATT

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MKPRLKMRLGDLLVQEAIITEAQLQQALGEQRNTGKKLGRTLIDLQSITEHQLLQFLSQQLNVPLLDISKMPIPAEVVHL
IPEVQARRFRALAVEDRGDSVLVAMSDPTDLQALDHLEMLIAPKRLKLAVAPEQQLLQAFDNLYRRTDQIAQIAGKLEEE
YAADEMFDLASLTSGDSDNETTVVKLLQSIFEDAVQMRASDIHIEPGDKALRIRQRIDGQLHETILNEVNIAAALVLRLK
LMAGLDISEKRLPQDGRFHMEIKGHHIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETGMPPAILARIRKQIKRPHGML
LVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLPRINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDQET
VEIGLRGALTGHFVLSTLHTNDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCHNCGVEYQPTSQEKAWLSSVSRK
DFTHANFRIGTGCQSCNGSGYRGRIGIFEILELDEKMIDAMRTGNPQDFARAALQSPNFTPLAESALQYLSEGMTTIEEV
AKLVEDVSESQIPLSKDIISQQGVEA

Sequences:

>Translated_586_residues
MKPRLKMRLGDLLVQEAIITEAQLQQALGEQRNTGKKLGRTLIDLQSITEHQLLQFLSQQLNVPLLDISKMPIPAEVVHL
IPEVQARRFRALAVEDRGDSVLVAMSDPTDLQALDHLEMLIAPKRLKLAVAPEQQLLQAFDNLYRRTDQIAQIAGKLEEE
YAADEMFDLASLTSGDSDNETTVVKLLQSIFEDAVQMRASDIHIEPGDKALRIRQRIDGQLHETILNEVNIAAALVLRLK
LMAGLDISEKRLPQDGRFHMEIKGHHIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETGMPPAILARIRKQIKRPHGML
LVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLPRINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDQET
VEIGLRGALTGHFVLSTLHTNDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCHNCGVEYQPTSQEKAWLSSVSRK
DFTHANFRIGTGCQSCNGSGYRGRIGIFEILELDEKMIDAMRTGNPQDFARAALQSPNFTPLAESALQYLSEGMTTIEEV
AKLVEDVSESQIPLSKDIISQQGVEA
>Mature_586_residues
MKPRLKMRLGDLLVQEAIITEAQLQQALGEQRNTGKKLGRTLIDLQSITEHQLLQFLSQQLNVPLLDISKMPIPAEVVHL
IPEVQARRFRALAVEDRGDSVLVAMSDPTDLQALDHLEMLIAPKRLKLAVAPEQQLLQAFDNLYRRTDQIAQIAGKLEEE
YAADEMFDLASLTSGDSDNETTVVKLLQSIFEDAVQMRASDIHIEPGDKALRIRQRIDGQLHETILNEVNIAAALVLRLK
LMAGLDISEKRLPQDGRFHMEIKGHHIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETGMPPAILARIRKQIKRPHGML
LVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLPRINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDQET
VEIGLRGALTGHFVLSTLHTNDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCHNCGVEYQPTSQEKAWLSSVSRK
DFTHANFRIGTGCQSCNGSGYRGRIGIFEILELDEKMIDAMRTGNPQDFARAALQSPNFTPLAESALQYLSEGMTTIEEV
AKLVEDVSESQIPLSKDIISQQGVEA

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=418, Percent_Identity=44.7368421052632, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1786296, Length=488, Percent_Identity=37.0901639344262, Blast_Score=299, Evalue=3e-82,
Organism=Escherichia coli, GI87082188, Length=277, Percent_Identity=33.2129963898917, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 65167; Mature: 65167

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRLKMRLGDLLVQEAIITEAQLQQALGEQRNTGKKLGRTLIDLQSITEHQLLQFLSQQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVPLLDISKMPIPAEVVHLIPEVQARRFRALAVEDRGDSVLVAMSDPTDLQALDHLEML
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHH
IAPKRLKLAVAPEQQLLQAFDNLYRRTDQIAQIAGKLEEEYAADEMFDLASLTSGDSDNE
HCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TTVVKLLQSIFEDAVQMRASDIHIEPGDKALRIRQRIDGQLHETILNEVNIAAALVLRLK
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LMAGLDISEKRLPQDGRFHMEIKGHHIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETG
HHHCCCCHHHHCCCCCCEEEEEECEEEEEEEEEECEECCHHHHHHHHHCCCCEEEECCCC
MPPAILARIRKQIKRPHGMLLVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLP
CCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
RINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDQETVEIGLRGALTGHFVLSTLHT
CCCEEEECCEECCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
NDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCHNCGVEYQPTSQEKAWLSSVSRK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
DFTHANFRIGTGCQSCNGSGYRGRIGIFEILELDEKMIDAMRTGNPQDFARAALQSPNFT
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
PLAESALQYLSEGMTTIEEVAKLVEDVSESQIPLSKDIISQQGVEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
>Mature Secondary Structure
MKPRLKMRLGDLLVQEAIITEAQLQQALGEQRNTGKKLGRTLIDLQSITEHQLLQFLSQQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LNVPLLDISKMPIPAEVVHLIPEVQARRFRALAVEDRGDSVLVAMSDPTDLQALDHLEML
CCCCEEECCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEEEECCCCHHHHHHHHHHH
IAPKRLKLAVAPEQQLLQAFDNLYRRTDQIAQIAGKLEEEYAADEMFDLASLTSGDSDNE
HCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
TTVVKLLQSIFEDAVQMRASDIHIEPGDKALRIRQRIDGQLHETILNEVNIAAALVLRLK
HHHHHHHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
LMAGLDISEKRLPQDGRFHMEIKGHHIDVRMSTMPIYHGESVVMRLLDQSAGLLTLNETG
HHHCCCCHHHHCCCCCCEEEEEECEEEEEEEEEECEECCHHHHHHHHHCCCCEEEECCCC
MPPAILARIRKQIKRPHGMLLVTGPTGSGKTTTLYGILSELNTADRKIITVEDPVEYQLP
CCHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCC
RINQVQVNHKIGLNFSNVLRTTLRQDPDIIMVGEMRDQETVEIGLRGALTGHFVLSTLHT
CCCEEEECCEECCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHC
NDAVTSALRLLDMGAASYLVASALRVIIAQRLVRRVCHNCGVEYQPTSQEKAWLSSVSRK
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
DFTHANFRIGTGCQSCNGSGYRGRIGIFEILELDEKMIDAMRTGNPQDFARAALQSPNFT
CCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCC
PLAESALQYLSEGMTTIEEVAKLVEDVSESQIPLSKDIISQQGVEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]