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The map label for this gene is yafV [C]

Identifier: 126176007

GI number: 126176007

Start: 4482727

End: 4483557

Strand: Reverse

Name: yafV [C]

Synonym: Sbal_3816

Alternate gene names: 126176007

Gene position: 4483557-4482727 (Counterclockwise)

Preceding gene: 126176008

Following gene: 126176006

Centisome position: 87.44

GC content: 49.7

Gene sequence:

>831_bases
ATGCACATCAGCCTGTTGCAATGTCAGAGCAGTCGCGACGTCAGCGCCAATCTGCTGTTTATCGAATCGCAGCTTAACGA
ACTCAAACGGGATGTTGATGCGCCCCATTTAGTCGTGTTGCCCGAATGCAGTTTACTGTTTGGTGGCCACGAAAGTCAGC
AACTCGCCTATGCGGGTGATGCTCATCAGAGTCCGCTCAAATCGGCGTTAAGTGCATTGGCCGCCAAATATCAGGTATTT
ATGGTGGCAGGCACTATTCCGGCGCTGGCTGAGGATGGCCGCGTCTATAGCCGCAGTTATCTGTTTGATGATAAAGGTGA
TACTTTAGGGTATTACGATAAGCTGCATCTCTTTGATGTGGATGTTGCCGATGGCACAAAACAATATCGTGAGAGCGAAA
CCTTCTGCCCCGGTGATCACATTAGCGTTATCGATACACCTTTTGGCAAAATTGGCCTAGCCATCTGTTATGATTTACGC
TTTCCCGACTTATTTAGGGCGATGCGTCTTGCTGGGGCCGAGATCATTACCTTGCCGTCGGCCTTTACCAAAGTGACAGG
TGAAGCCCATTGGCAAGTATTGCTACAGGCAAGAGCCATTGAAACCCAATGCGTTATTCTTGCTGCAGCTCAGTGGGGAC
AGCATAACGAAGGCAGTCGTGAAACTTGGGGGCAGAGCATGGTAGTCGGTCCTTGGGGCAATATTTTGGCTGAACGTAAA
ACGGGTACGGGTTGGGTGCAGGCTGAGGTCGATTTAACCGAACTGCACAGTATTCGCCGTAAAATGCCCGTGATGCAGCA
TAATCGTTTCCTTGAGCCAAGCTTGAAATAA

Upstream 100 bases:

>100_bases
CAACCTCAAGCAACGCCTTCGCTTGAAGTACCGAACACGCCCGCCACACCAGAGAATCAGCCAGCAGTAGAACCGATTAA
GCAAATTCAGGGAGATCAGA

Downstream 100 bases:

>100_bases
CCGCCCGCACTGGCGAGCGGTAATACCGAATTAGACAGCGATTTACGTACAGAATTAGTACAGCGAATTTACACTCAGAA
TATCAAGATCCCTTGGGGTC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MHISLLQCQSSRDVSANLLFIESQLNELKRDVDAPHLVVLPECSLLFGGHESQQLAYAGDAHQSPLKSALSALAAKYQVF
MVAGTIPALAEDGRVYSRSYLFDDKGDTLGYYDKLHLFDVDVADGTKQYRESETFCPGDHISVIDTPFGKIGLAICYDLR
FPDLFRAMRLAGAEIITLPSAFTKVTGEAHWQVLLQARAIETQCVILAAAQWGQHNEGSRETWGQSMVVGPWGNILAERK
TGTGWVQAEVDLTELHSIRRKMPVMQHNRFLEPSLK

Sequences:

>Translated_276_residues
MHISLLQCQSSRDVSANLLFIESQLNELKRDVDAPHLVVLPECSLLFGGHESQQLAYAGDAHQSPLKSALSALAAKYQVF
MVAGTIPALAEDGRVYSRSYLFDDKGDTLGYYDKLHLFDVDVADGTKQYRESETFCPGDHISVIDTPFGKIGLAICYDLR
FPDLFRAMRLAGAEIITLPSAFTKVTGEAHWQVLLQARAIETQCVILAAAQWGQHNEGSRETWGQSMVVGPWGNILAERK
TGTGWVQAEVDLTELHSIRRKMPVMQHNRFLEPSLK
>Mature_276_residues
MHISLLQCQSSRDVSANLLFIESQLNELKRDVDAPHLVVLPECSLLFGGHESQQLAYAGDAHQSPLKSALSALAAKYQVF
MVAGTIPALAEDGRVYSRSYLFDDKGDTLGYYDKLHLFDVDVADGTKQYRESETFCPGDHISVIDTPFGKIGLAICYDLR
FPDLFRAMRLAGAEIITLPSAFTKVTGEAHWQVLLQARAIETQCVILAAAQWGQHNEGSRETWGQSMVVGPWGNILAERK
TGTGWVQAEVDLTELHSIRRKMPVMQHNRFLEPSLK

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=177, Percent_Identity=49.7175141242938, Blast_Score=177, Evalue=8e-45,
Organism=Homo sapiens, GI297632350, Length=177, Percent_Identity=49.7175141242938, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI297632348, Length=177, Percent_Identity=49.7175141242938, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI9910460, Length=272, Percent_Identity=34.1911764705882, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI297632346, Length=100, Percent_Identity=47, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1786412, Length=256, Percent_Identity=29.296875, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556280, Length=273, Percent_Identity=36.6300366300366, Blast_Score=164, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6323383, Length=280, Percent_Identity=35.7142857142857, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6322335, Length=299, Percent_Identity=34.7826086956522, Blast_Score=159, Evalue=5e-40,
Organism=Drosophila melanogaster, GI17933642, Length=273, Percent_Identity=39.1941391941392, Blast_Score=178, Evalue=3e-45,
Organism=Drosophila melanogaster, GI21355835, Length=275, Percent_Identity=33.8181818181818, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30690; Mature: 30690

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHISLLQCQSSRDVSANLLFIESQLNELKRDVDAPHLVVLPECSLLFGGHESQQLAYAGD
CCEEEEECCCCCCCCEEEEEEHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCEEEECCC
AHQSPLKSALSALAAKYQVFMVAGTIPALAEDGRVYSRSYLFDDKGDTLGYYDKLHLFDV
CCHHHHHHHHHHHHHHEEEEEEECCCCHHHCCCCEEEEEEEECCCCCCCCCEEEEEEEEE
DVADGTKQYRESETFCPGDHISVIDTPFGKIGLAICYDLRFPDLFRAMRLAGAEIITLPS
ECCCCHHHHHCCCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCCEEEECCH
AFTKVTGEAHWQVLLQARAIETQCVILAAAQWGQHNEGSRETWGQSMVVGPWGNILAERK
HHHHHCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHHHCCCEEEECCCHHHHEECC
TGTGWVQAEVDLTELHSIRRKMPVMQHNRFLEPSLK
CCCCEEEEECCHHHHHHHHHHCCHHHCCCCCCCCCC
>Mature Secondary Structure
MHISLLQCQSSRDVSANLLFIESQLNELKRDVDAPHLVVLPECSLLFGGHESQQLAYAGD
CCEEEEECCCCCCCCEEEEEEHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCEEEECCC
AHQSPLKSALSALAAKYQVFMVAGTIPALAEDGRVYSRSYLFDDKGDTLGYYDKLHLFDV
CCHHHHHHHHHHHHHHEEEEEEECCCCHHHCCCCEEEEEEEECCCCCCCCCEEEEEEEEE
DVADGTKQYRESETFCPGDHISVIDTPFGKIGLAICYDLRFPDLFRAMRLAGAEIITLPS
ECCCCHHHHHCCCCCCCCCCEEEEECCCCCEEEEEEEECCCHHHHHHHHHCCCEEEECCH
AFTKVTGEAHWQVLLQARAIETQCVILAAAQWGQHNEGSRETWGQSMVVGPWGNILAERK
HHHHHCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHHHCCCEEEECCCHHHHEECC
TGTGWVQAEVDLTELHSIRRKMPVMQHNRFLEPSLK
CCCCEEEEECCHHHHHHHHHHCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]