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The map label for this gene is rimO [H]

Identifier: 126175991

GI number: 126175991

Start: 4459926

End: 4461344

Strand: Direct

Name: rimO [H]

Synonym: Sbal_3800

Alternate gene names: 126175991

Gene position: 4459926-4461344 (Clockwise)

Preceding gene: 126175981

Following gene: 126175994

Centisome position: 86.98

GC content: 48.56

Gene sequence:

>1419_bases
ATGACAGTTGAAACTTTTAAACCCAAGCAAACGACCACGCTTGATATCCCAGTAAAAACATTAGAAGCAGCAAGCACTAA
TGCTGTTACCACTGGGAATCGCATCGGTTTCGTGTCTTTGGGCTGCCCAAAAAACCTCGTGGATTCAGAGCGTATTCTGA
CTCAGTTACGCATCGACGGCTACGAAGTGACTAACAGCTACGCCAATGCCGATCTGGTTATCGTCAACACCTGCGGCTTT
ATCGATGCTGCGGTTGAAGAATCTCTGGACGCTGTGCGTGAAGCCTTAGAAGAAAACGGCAAAGTGATCGTCACTGGCTG
CCTTGGCGCCAAAGAAAATCAAATCCGCGAAGTACATCCAGATGTGTTAGAAATCACTGGCCCACACAGCTACGAAGCTG
TGTTGAAGCACGTTCACAAATATGTGCCTAAGCCAGAACACAACCCTTTCACATCATTAATCCCACAAACTGGGGTTAAG
TTAACGCCTAAGCATTACGCTTATTTAAAGATTTCAGAAGGTTGCGACAACCGTTGTACTTTCTGCATTATTCCTTCTCT
ACGTGGCGATCTCGACAGCCGCCCAGCGGGCAGTATCTTAGATGAAGCTAAGCGTTTAGTTGAATCAGGCGTACAAGAAA
TTCTAGTCGTGAGCCAAGACACCTCGGCCTACGGCAAAGACAAAGGCGGTCGTACCGATTTTTGGAACGGTATGCCAGTC
AAGCAAGACATTACTAGCCTAGCGCGCCAACTGGGTAAAATGGGCGCTTGGGTGCGTCTGCATTATATCTATCCATACCC
ATGGGTTGACGATTTGATCCCGCTGATGGCCGAAGGTTTAATTCTGCCGTATTTGGATATTCCAATGCAGCATGCAAGCC
CACGCATCCTGAAGATGATGAAGCGTCCAGGCCGTGTTGACCGTCAGTTAGAAGCGATTCAACGCTGGCGTGAAATTTGT
CCCGATCTAGTGATCCGCTCGACCTTCATCGTCGGCTTCCCAGGTGAAACTGAAGAGGATTTCCAAATCCTGCTCGATTT
CTTAAAAGAAGCACGTCTTGATCGCGTAGGTTGCTTTAAGTATTCAGAAGTCGATGGCGCTGTCGCCAACACCATCGCCG
AGCTTATCAGCGAAGATGTTAAAGAAGACAGATACCACAGATTTATGGAAGTCCAAGCCGAGATCAGCGCCGAGCGTTTA
GCCCGTTTCGTTGGCCGTACCTTAGACATTCTGATTGACGATGTTGACGAAGAAGGCGCCATTGGCCGCAGCTTCGCCGA
TGCACCTGAAATCGACGGTATGGTGTTTATCAATGGTGAAACTGAGTTGGAACCTGGTATGTTGGTTCGTGCTCGCATCA
CCCATTCAGACGAACACGATCTGTGGGCTGAAGTAGTAGACGCTGACACCCAAGACTAA

Upstream 100 bases:

>100_bases
CCCAGTTGTACACTTTTCAGCCAAAGCTACTGTACCTTTTAACCACTTCAGGTATAATCCGCCATCTTTTTTAAGGCTGC
TCTAACCTCAGCGAGAAACC

Downstream 100 bases:

>100_bases
GCTGTTCTGATACAGGACAGGCTATGACTCAAAAAGCGAACCTCTGGTTCGCTTTTTGTTTTTTATCATTTCGTAAGCAT
TAACACTGCGAATGCTTCAC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTVETFKPKQTTTLDIPVKTLEAASTNAVTTGNRIGFVSLGCPKNLVDSERILTQLRIDGYEVTNSYANADLVIVNTCGF
IDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQTGVK
LTPKHYAYLKISEGCDNRCTFCIIPSLRGDLDSRPAGSILDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWNGMPV
KQDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRILKMMKRPGRVDRQLEAIQRWREIC
PDLVIRSTFIVGFPGETEEDFQILLDFLKEARLDRVGCFKYSEVDGAVANTIAELISEDVKEDRYHRFMEVQAEISAERL
ARFVGRTLDILIDDVDEEGAIGRSFADAPEIDGMVFINGETELEPGMLVRARITHSDEHDLWAEVVDADTQD

Sequences:

>Translated_472_residues
MTVETFKPKQTTTLDIPVKTLEAASTNAVTTGNRIGFVSLGCPKNLVDSERILTQLRIDGYEVTNSYANADLVIVNTCGF
IDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQTGVK
LTPKHYAYLKISEGCDNRCTFCIIPSLRGDLDSRPAGSILDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWNGMPV
KQDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRILKMMKRPGRVDRQLEAIQRWREIC
PDLVIRSTFIVGFPGETEEDFQILLDFLKEARLDRVGCFKYSEVDGAVANTIAELISEDVKEDRYHRFMEVQAEISAERL
ARFVGRTLDILIDDVDEEGAIGRSFADAPEIDGMVFINGETELEPGMLVRARITHSDEHDLWAEVVDADTQD
>Mature_471_residues
TVETFKPKQTTTLDIPVKTLEAASTNAVTTGNRIGFVSLGCPKNLVDSERILTQLRIDGYEVTNSYANADLVIVNTCGFI
DAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHPDVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQTGVKL
TPKHYAYLKISEGCDNRCTFCIIPSLRGDLDSRPAGSILDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWNGMPVK
QDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRILKMMKRPGRVDRQLEAIQRWREICP
DLVIRSTFIVGFPGETEEDFQILLDFLKEARLDRVGCFKYSEVDGAVANTIAELISEDVKEDRYHRFMEVQAEISAERLA
RFVGRTLDILIDDVDEEGAIGRSFADAPEIDGMVFINGETELEPGMLVRARITHSDEHDLWAEVVDADTQD

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=383, Percent_Identity=27.9373368146214, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI28872784, Length=462, Percent_Identity=25.5411255411255, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI28872782, Length=462, Percent_Identity=25.5411255411255, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1787057, Length=440, Percent_Identity=72.2727272727273, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=456, Percent_Identity=27.4122807017544, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17553146, Length=478, Percent_Identity=25.5230125523013, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71996771, Length=216, Percent_Identity=30.0925925925926, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21356207, Length=436, Percent_Identity=25.4587155963303, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI19922432, Length=473, Percent_Identity=24.7357293868922, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52802; Mature: 52671

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVETFKPKQTTTLDIPVKTLEAASTNAVTTGNRIGFVSLGCPKNLVDSERILTQLRIDG
CCCCCCCCCCCEEEECCHHHHHCCCCCCEECCCCEEEEEECCCCCCCHHHHHHHHHHCCC
YEVTNSYANADLVIVNTCGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHP
EEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCC
DVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQTGVKLTPKHYAYLKISEGCDNRCT
CEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCCEEEEEECCCCCCCCE
FCIIPSLRGDLDSRPAGSILDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWNGMPV
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCH
KQDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRILKMM
HHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHH
KRPGRVDRQLEAIQRWREICPDLVIRSTFIVGFPGETEEDFQILLDFLKEARLDRVGCFK
HCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEE
YSEVDGAVANTIAELISEDVKEDRYHRFMEVQAEISAERLARFVGRTLDILIDDVDEEGA
EHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCC
IGRSFADAPEIDGMVFINGETELEPGMLVRARITHSDEHDLWAEVVDADTQD
CCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
TVETFKPKQTTTLDIPVKTLEAASTNAVTTGNRIGFVSLGCPKNLVDSERILTQLRIDG
CCCCCCCCCCEEEECCHHHHHCCCCCCEECCCCEEEEEECCCCCCCHHHHHHHHHHCCC
YEVTNSYANADLVIVNTCGFIDAAVEESLDAVREALEENGKVIVTGCLGAKENQIREVHP
EEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCC
DVLEITGPHSYEAVLKHVHKYVPKPEHNPFTSLIPQTGVKLTPKHYAYLKISEGCDNRCT
CEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCCEEEEEECCCCCCCCE
FCIIPSLRGDLDSRPAGSILDEAKRLVESGVQEILVVSQDTSAYGKDKGGRTDFWNGMPV
EEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCCCCCCH
KQDITSLARQLGKMGAWVRLHYIYPYPWVDDLIPLMAEGLILPYLDIPMQHASPRILKMM
HHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHH
KRPGRVDRQLEAIQRWREICPDLVIRSTFIVGFPGETEEDFQILLDFLKEARLDRVGCFK
HCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEE
YSEVDGAVANTIAELISEDVKEDRYHRFMEVQAEISAERLARFVGRTLDILIDDVDEEGA
EHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCCC
IGRSFADAPEIDGMVFINGETELEPGMLVRARITHSDEHDLWAEVVDADTQD
CCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]