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The map label for this gene is gph2 [H]

Identifier: 126175954

GI number: 126175954

Start: 4414670

End: 4415284

Strand: Reverse

Name: gph2 [H]

Synonym: Sbal_3760

Alternate gene names: 126175954

Gene position: 4415284-4414670 (Counterclockwise)

Preceding gene: 126175955

Following gene: 126175952

Centisome position: 86.11

GC content: 50.73

Gene sequence:

>615_bases
GTGATCACTAGACTTAGCGATTTAAAACTTAAGGATGTCCGTGGGGTCATCTTCGATCTTGACGGCACTTTAGCGCATTC
CAATCCCGATTTTGCTGGTCTGCGGCGTGAGTTGGGGATTCATTCTGGTACGGATATCTTGGCCCATGTGGATAGCCTGA
GTGCGGGTGAAGCACAGGCTCAGGCGCTGGAAATCGTCCATGAGTACGAGAGACAAAGCTCACTCAATGCCAGTTGGATT
GATGGCGTGCGTGAGCTTATCGAATGCCTACGGGCTAAGTCGCTGCCCTTGGCTATTTTGACCCGCAATATGCCGGAAGC
GGCGCAAATCACTATCGATAAGCTTGGCATAGATATCGAACTAGTATTAACGCGTTACGACGCGCAGCCTAAGCCGCATC
CCGAAGGTATTCATCTTATTTGCCAGCAGTGGCAACTCGCCCCAGCAGATATCTTATATGTGGGCGATTATTTGTTTGAT
CTGCAAACTGCGCGTAATGCGGGTAGCCGCTGTGCTCTCTATTGTCCTGAGGATATTCCCGATTATGCCCACGACGCCGA
CATGCTAGTGGCTTGCTACCATAGCTTTATCGAAGCGTGGACAGTGGGTGACTAA

Upstream 100 bases:

>100_bases
GCGAAGCGTTTGCAGGCTGCCTAATATCCATGGTATTTGATTAGCCCAATGGTATTTAATTAGCGTATGAAACATACATT
TTCACCCATGGGAAAGGCCA

Downstream 100 bases:

>100_bases
GCATTGTTTGGGGCTGCATCTTGCTTATCTTGCTTCAGTCCCAAGCGTACACTATCCAATAAGGCAATCAGCCAGCAGAG
GATAAAGCCGTACATCAGTT

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MITRLSDLKLKDVRGVIFDLDGTLAHSNPDFAGLRRELGIHSGTDILAHVDSLSAGEAQAQALEIVHEYERQSSLNASWI
DGVRELIECLRAKSLPLAILTRNMPEAAQITIDKLGIDIELVLTRYDAQPKPHPEGIHLICQQWQLAPADILYVGDYLFD
LQTARNAGSRCALYCPEDIPDYAHDADMLVACYHSFIEAWTVGD

Sequences:

>Translated_204_residues
MITRLSDLKLKDVRGVIFDLDGTLAHSNPDFAGLRRELGIHSGTDILAHVDSLSAGEAQAQALEIVHEYERQSSLNASWI
DGVRELIECLRAKSLPLAILTRNMPEAAQITIDKLGIDIELVLTRYDAQPKPHPEGIHLICQQWQLAPADILYVGDYLFD
LQTARNAGSRCALYCPEDIPDYAHDADMLVACYHSFIEAWTVGD
>Mature_204_residues
MITRLSDLKLKDVRGVIFDLDGTLAHSNPDFAGLRRELGIHSGTDILAHVDSLSAGEAQAQALEIVHEYERQSSLNASWI
DGVRELIECLRAKSLPLAILTRNMPEAAQITIDKLGIDIELVLTRYDAQPKPHPEGIHLICQQWQLAPADILYVGDYLFD
LQTARNAGSRCALYCPEDIPDYAHDADMLVACYHSFIEAWTVGD

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324705, Length=180, Percent_Identity=26.1111111111111, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 22666; Mature: 22666

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRLSDLKLKDVRGVIFDLDGTLAHSNPDFAGLRRELGIHSGTDILAHVDSLSAGEAQA
CCCCCCCCCHHHHCEEEEECCCEEECCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHH
QALEIVHEYERQSSLNASWIDGVRELIECLRAKSLPLAILTRNMPEAAQITIDKLGIDIE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHEEEHHHHCCEEE
LVLTRYDAQPKPHPEGIHLICQQWQLAPADILYVGDYLFDLQTARNAGSRCALYCPEDIP
EEEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCCC
DYAHDADMLVACYHSFIEAWTVGD
CCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MITRLSDLKLKDVRGVIFDLDGTLAHSNPDFAGLRRELGIHSGTDILAHVDSLSAGEAQA
CCCCCCCCCHHHHCEEEEECCCEEECCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCHHHH
QALEIVHEYERQSSLNASWIDGVRELIECLRAKSLPLAILTRNMPEAAQITIDKLGIDIE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHEEEHHHHCCEEE
LVLTRYDAQPKPHPEGIHLICQQWQLAPADILYVGDYLFDLQTARNAGSRCALYCPEDIP
EEEEECCCCCCCCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCEEEEECCCCCC
DYAHDADMLVACYHSFIEAWTVGD
CCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]