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The map label for this gene is dcuA [H]

Identifier: 126175942

GI number: 126175942

Start: 4397709

End: 4399010

Strand: Direct

Name: dcuA [H]

Synonym: Sbal_3748

Alternate gene names: 126175942

Gene position: 4397709-4399010 (Clockwise)

Preceding gene: 126175941

Following gene: 126175943

Centisome position: 85.77

GC content: 49.39

Gene sequence:

>1302_bases
ATGATTATATTAGAGATAATTATCGTACTCGGGGCCATTTACCTAGGTGCAAGGATGGGTAGCATGGGAGTCGGCTTTGC
GGGCGGTGTCGGGGTACTGATACTGACCGCAGGGATGGGACTCAAACCGGGCAATATTCCGATTGATGTGATCCTCATCA
TTATGTCGGTTATCACCGCGATTGCGGCCATGCAAGTATCAGGAGGGATGGATTATTTAGTCTCACTGGCAGAAAAATTA
TTACGTAAGCATCCAAAACAAATCACTTTTCTGGCACCTGTAGTCACTTATTTCATGACACTCTTTGCAGGAACAGGCCA
CACCGCATTCTCAACCTTACCTGTTATTGCCGAAGTCGCAAAAGAGCAAGGTATTCGTCCCTCTCGTCCTCTTTCTATCG
CCGTTGTCGCGTCCCAAGTCGCCATTACCGCTTCACCGATTTCGGCGGCTGTCGTGTTCTTCTCAGGCATTCTAGAACCC
TTTGGCGTGGACTATTTAGTACTGTTAGCCGTATGTATTCCTTCAACTTTCTTAGCCTGTATGGTCGGCGCCGTTGTTGC
TAACTTAATGGGTAAAGAGCTGGCTGATGATCCTATTTATCAAGACCGTTTAGCTAAGGGCTTAGTCAAAACACGCGGTA
AAACTGAGATCAAGATTAAAGCCGGCGCAAAACGATCTGTGATGATTTTCTTGCTGGCGATTGCGGCTGTGATGGCTTAT
GCCACTGCGATATCTGACAAAGTCGGCTTGATTGTTGACCCTGTACTGCCACGCGATGCCGCCATTATGGTATTGATGCT
AACGGCAGCTGCGGCTATCACGATTTTCTGTAAGTTAGACGCAAGTGAAATCTCCAATGCGGCCACCTTTAAATCGGGCA
TGTCAGCCTGTGTTTGTGTGTTAGGTGTGGCTTGGTTAGGCGATACCTTAGTGTCGAATCATATTGAGGAAATCAAAGAT
TTAGCAGGCACCTTACTGCAAAGTCAGCCTTGGTTATTGTCGGTAACCCTGTTCTTTGCCTCCATGTTGCTCTACTCCCA
AGGCGCAACCACTAAGGCACTGATGCCTGCGGCCTTAGCCATTGGCGTGAGTCCTTTAACTGCCGTTGCCTCTTTCGCCG
CCGTCAGCGCGCTGTTTGTACTGCCCACTTATCCCACGCTACTGGCTGCGGTTGAGATGGATGATACCGGCTCGACGCGG
ATTGGTAAGGCGGTATTTAACCATCCGTTCTTGGTCCCTGGCGTAGTGACGATTGCCACCAGCGTGGCATTGGCCTTCCT
GTTTGGCGGCATGATGCTGTAG

Upstream 100 bases:

>100_bases
GGAGCCTTCCCCAGCCCAAAACTCGATGATTCAAGAATAAGCCAGCGCATAGTGAATATGCGCAGGCTATTGAAATTAAA
AACAAAATAGAGTTAACACT

Downstream 100 bases:

>100_bases
CGCAACTTTACCCAAAGTGTTCTAAGGGCTAATGCCAATAATTGGATTAACCCTCTTCTTTTAATCTGGATCTAAAGACT
TTGTGTTGGCGTGATGCAGG

Product: anaerobic c4-dicarboxylate antiporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MIILEIIIVLGAIYLGARMGSMGVGFAGGVGVLILTAGMGLKPGNIPIDVILIIMSVITAIAAMQVSGGMDYLVSLAEKL
LRKHPKQITFLAPVVTYFMTLFAGTGHTAFSTLPVIAEVAKEQGIRPSRPLSIAVVASQVAITASPISAAVVFFSGILEP
FGVDYLVLLAVCIPSTFLACMVGAVVANLMGKELADDPIYQDRLAKGLVKTRGKTEIKIKAGAKRSVMIFLLAIAAVMAY
ATAISDKVGLIVDPVLPRDAAIMVLMLTAAAAITIFCKLDASEISNAATFKSGMSACVCVLGVAWLGDTLVSNHIEEIKD
LAGTLLQSQPWLLSVTLFFASMLLYSQGATTKALMPAALAIGVSPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTR
IGKAVFNHPFLVPGVVTIATSVALAFLFGGMML

Sequences:

>Translated_433_residues
MIILEIIIVLGAIYLGARMGSMGVGFAGGVGVLILTAGMGLKPGNIPIDVILIIMSVITAIAAMQVSGGMDYLVSLAEKL
LRKHPKQITFLAPVVTYFMTLFAGTGHTAFSTLPVIAEVAKEQGIRPSRPLSIAVVASQVAITASPISAAVVFFSGILEP
FGVDYLVLLAVCIPSTFLACMVGAVVANLMGKELADDPIYQDRLAKGLVKTRGKTEIKIKAGAKRSVMIFLLAIAAVMAY
ATAISDKVGLIVDPVLPRDAAIMVLMLTAAAAITIFCKLDASEISNAATFKSGMSACVCVLGVAWLGDTLVSNHIEEIKD
LAGTLLQSQPWLLSVTLFFASMLLYSQGATTKALMPAALAIGVSPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTR
IGKAVFNHPFLVPGVVTIATSVALAFLFGGMML
>Mature_433_residues
MIILEIIIVLGAIYLGARMGSMGVGFAGGVGVLILTAGMGLKPGNIPIDVILIIMSVITAIAAMQVSGGMDYLVSLAEKL
LRKHPKQITFLAPVVTYFMTLFAGTGHTAFSTLPVIAEVAKEQGIRPSRPLSIAVVASQVAITASPISAAVVFFSGILEP
FGVDYLVLLAVCIPSTFLACMVGAVVANLMGKELADDPIYQDRLAKGLVKTRGKTEIKIKAGAKRSVMIFLLAIAAVMAY
ATAISDKVGLIVDPVLPRDAAIMVLMLTAAAAITIFCKLDASEISNAATFKSGMSACVCVLGVAWLGDTLVSNHIEEIKD
LAGTLLQSQPWLLSVTLFFASMLLYSQGATTKALMPAALAIGVSPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTR
IGKAVFNHPFLVPGVVTIATSVALAFLFGGMML

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=434, Percent_Identity=61.7511520737327, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1790565, Length=402, Percent_Identity=36.5671641791045, Blast_Score=241, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45042; Mature: 45042

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIILEIIIVLGAIYLGARMGSMGVGFAGGVGVLILTAGMGLKPGNIPIDVILIIMSVITA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHHHHHH
IAAMQVSGGMDYLVSLAEKLLRKHPKQITFLAPVVTYFMTLFAGTGHTAFSTLPVIAEVA
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KEQGIRPSRPLSIAVVASQVAITASPISAAVVFFSGILEPFGVDYLVLLAVCIPSTFLAC
HHCCCCCCCCEEEEEEHHHHHEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
MVGAVVANLMGKELADDPIYQDRLAKGLVKTRGKTEIKIKAGAKRSVMIFLLAIAAVMAY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
ATAISDKVGLIVDPVLPRDAAIMVLMLTAAAAITIFCKLDASEISNAATFKSGMSACVCV
HHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHH
LGVAWLGDTLVSNHIEEIKDLAGTLLQSQPWLLSVTLFFASMLLYSQGATTKALMPAALA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IGVSPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGKAVFNHPFLVPGVVTIAT
HCCHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHH
SVALAFLFGGMML
HHHHHHHHHHCCC
>Mature Secondary Structure
MIILEIIIVLGAIYLGARMGSMGVGFAGGVGVLILTAGMGLKPGNIPIDVILIIMSVITA
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHHHHHHH
IAAMQVSGGMDYLVSLAEKLLRKHPKQITFLAPVVTYFMTLFAGTGHTAFSTLPVIAEVA
HHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
KEQGIRPSRPLSIAVVASQVAITASPISAAVVFFSGILEPFGVDYLVLLAVCIPSTFLAC
HHCCCCCCCCEEEEEEHHHHHEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
MVGAVVANLMGKELADDPIYQDRLAKGLVKTRGKTEIKIKAGAKRSVMIFLLAIAAVMAY
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHH
ATAISDKVGLIVDPVLPRDAAIMVLMLTAAAAITIFCKLDASEISNAATFKSGMSACVCV
HHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHH
LGVAWLGDTLVSNHIEEIKDLAGTLLQSQPWLLSVTLFFASMLLYSQGATTKALMPAALA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
IGVSPLTAVASFAAVSALFVLPTYPTLLAAVEMDDTGSTRIGKAVFNHPFLVPGVVTIAT
HCCHHHHHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCHHHHHHHCCCCHHHHHHHHHH
SVALAFLFGGMML
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11004174; 14500908; 8898907 [H]