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The map label for this gene is sepA [H]

Identifier: 126175906

GI number: 126175906

Start: 4338241

End: 4339344

Strand: Direct

Name: sepA [H]

Synonym: Sbal_3712

Alternate gene names: 126175906

Gene position: 4338241-4339344 (Clockwise)

Preceding gene: 126175904

Following gene: 126175907

Centisome position: 84.61

GC content: 50.18

Gene sequence:

>1104_bases
ATGAAATCATATCGACTCTTGCCACTGGCTATGCTGCTTCCAGACCTGCTCAGTGGTTGTTCGCCTGCTGAAAGTTCAGT
ACAAGTGGACACTGTCCGTCCGGTTAAACTGTTTGAAGTGCTCAATGTAGAAGCTGGAGATTTTAGAACCTTTCCAGCCC
GCGTTTCCGCTAACAGCCGCGCCGAATTGTCGTTCCGTATTTCTGGGCAATTAACCGAACTGACATTAGTCGAGGGCCAA
AGAGTCGCTCAAGGTAGCCTGCTCGCGCAGCTTGACGACAGGGATGCCAAAAATAACTTAATGACCCGAGAGGCCGAACA
CGAACTGTTAGCCGCCGACTTTAAACGTAAGGTCGAGCTGCTCAATCGTAAGCTGATTTCACAATCTGAGTTTGATAGCA
CTCAAGCGCAGCTCAAATCGGCCAAGGCCGCCCTTGCGGCAGCACGCGATCAGCTGAGTTACACCCGCTTAACCGCGCCC
TTTAGCGGCACGATTGCTAAACGTTTAGTGGATAACCACCAAATAGTGCAGGCCAATCAAGGGGTGTTAACACTGCAAAA
CAACAACTTGCTCGATGTGAGCATTCAAGTTCCCGAGTCGATGGCGGCGGGGCTGAAGCAATATACCGATCAAGCTCACT
TTACCGCTAAGGTGCGTTTTAGCGCCTTTCCCGAGCAGAGTTTCGACGCCAAGTTTAAAGAATACTCCACCCAAGTCACC
CCAGGTACTCAAGCCTATGAAGTGGTATTTTCATTGCCTCAACCTCAAGATATTCAATTACTTCCGGGGATGAGCGCCGA
GCTAACCTTGGCCTTAGTTAAAGATCCAAGCGCCCTTGCATCCAGCATAGTGCCCATGACTGCCATCGATAAACGCGATC
AAGATGGCCAAGTGCAAGTGTGGGTTTATCAAACCCAATCGGGTGAAGTCAAACCCACTATGGTGACCCTAGGACGCGTG
AGTAACCAAGGCGTTGAGATTTTAACGGGTATCAACAAGGGCGATCTTATCGTCAGCGCTGGCGTGTCTCAATTATCCGC
TGGCATGAAAGTCAAACCACTACTATTGCGCGACAATTACCCTGACCGGTTCAGCGACAATTAG

Upstream 100 bases:

>100_bases
GGGACTGATAATCCCAAAGTATCCCGTTTATTGCAACTACTTTAGCTGGTATCTTCGCGCCCAGAAATGCTGACAACTTG
TTAACTAGGAAATCGCCACC

Downstream 100 bases:

>100_bases
AATGGCCGGTTGATCTGATACATTCTGGACAAAGATTCTAAGGGAGTTCTTTGTGCCAGGTCATCGAATTACAGATCAAC
AAATAAGGCTATTTATGTCT

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKSYRLLPLAMLLPDLLSGCSPAESSVQVDTVRPVKLFEVLNVEAGDFRTFPARVSANSRAELSFRISGQLTELTLVEGQ
RVAQGSLLAQLDDRDAKNNLMTREAEHELLAADFKRKVELLNRKLISQSEFDSTQAQLKSAKAALAAARDQLSYTRLTAP
FSGTIAKRLVDNHQIVQANQGVLTLQNNNLLDVSIQVPESMAAGLKQYTDQAHFTAKVRFSAFPEQSFDAKFKEYSTQVT
PGTQAYEVVFSLPQPQDIQLLPGMSAELTLALVKDPSALASSIVPMTAIDKRDQDGQVQVWVYQTQSGEVKPTMVTLGRV
SNQGVEILTGINKGDLIVSAGVSQLSAGMKVKPLLLRDNYPDRFSDN

Sequences:

>Translated_367_residues
MKSYRLLPLAMLLPDLLSGCSPAESSVQVDTVRPVKLFEVLNVEAGDFRTFPARVSANSRAELSFRISGQLTELTLVEGQ
RVAQGSLLAQLDDRDAKNNLMTREAEHELLAADFKRKVELLNRKLISQSEFDSTQAQLKSAKAALAAARDQLSYTRLTAP
FSGTIAKRLVDNHQIVQANQGVLTLQNNNLLDVSIQVPESMAAGLKQYTDQAHFTAKVRFSAFPEQSFDAKFKEYSTQVT
PGTQAYEVVFSLPQPQDIQLLPGMSAELTLALVKDPSALASSIVPMTAIDKRDQDGQVQVWVYQTQSGEVKPTMVTLGRV
SNQGVEILTGINKGDLIVSAGVSQLSAGMKVKPLLLRDNYPDRFSDN
>Mature_367_residues
MKSYRLLPLAMLLPDLLSGCSPAESSVQVDTVRPVKLFEVLNVEAGDFRTFPARVSANSRAELSFRISGQLTELTLVEGQ
RVAQGSLLAQLDDRDAKNNLMTREAEHELLAADFKRKVELLNRKLISQSEFDSTQAQLKSAKAALAAARDQLSYTRLTAP
FSGTIAKRLVDNHQIVQANQGVLTLQNNNLLDVSIQVPESMAAGLKQYTDQAHFTAKVRFSAFPEQSFDAKFKEYSTQVT
PGTQAYEVVFSLPQPQDIQLLPGMSAELTLALVKDPSALASSIVPMTAIDKRDQDGQVQVWVYQTQSGEVKPTMVTLGRV
SNQGVEILTGINKGDLIVSAGVSQLSAGMKVKPLLLRDNYPDRFSDN

Specific function: Probable periplasmic linker protein component of the sepABC efflux pump with unknown specificity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=26.4797507788162, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI87081796, Length=350, Percent_Identity=25.4285714285714, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789665, Length=377, Percent_Identity=26.2599469496021, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789929, Length=333, Percent_Identity=23.7237237237237, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786668, Length=332, Percent_Identity=25.9036144578313, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40213; Mature: 40213

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSYRLLPLAMLLPDLLSGCSPAESSVQVDTVRPVKLFEVLNVEAGDFRTFPARVSANSR
CCCCEEHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEHHHCCCCCCCEECCCEECCCCC
AELSFRISGQLTELTLVEGQRVAQGSLLAQLDDRDAKNNLMTREAEHELLAADFKRKVEL
EEEEEEECCCEEEEEEECCCCHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LNRKLISQSEFDSTQAQLKSAKAALAAARDQLSYTRLTAPFSGTIAKRLVDNHQIVQANQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCEEEEECC
GVLTLQNNNLLDVSIQVPESMAAGLKQYTDQAHFTAKVRFSAFPEQSFDAKFKEYSTQVT
CEEEEECCCEEEEEEECCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHCCCCC
PGTQAYEVVFSLPQPQDIQLLPGMSAELTLALVKDPSALASSIVPMTAIDKRDQDGQVQV
CCCCEEEEEEECCCCCCEEECCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEE
WVYQTQSGEVKPTMVTLGRVSNQGVEILTGINKGDLIVSAGVSQLSAGMKVKPLLLRDNY
EEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEECCHHHHHCCCEEEEEEEECCC
PDRFSDN
CCCCCCC
>Mature Secondary Structure
MKSYRLLPLAMLLPDLLSGCSPAESSVQVDTVRPVKLFEVLNVEAGDFRTFPARVSANSR
CCCCEEHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEHHHCCCCCCCEECCCEECCCCC
AELSFRISGQLTELTLVEGQRVAQGSLLAQLDDRDAKNNLMTREAEHELLAADFKRKVEL
EEEEEEECCCEEEEEEECCCCHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LNRKLISQSEFDSTQAQLKSAKAALAAARDQLSYTRLTAPFSGTIAKRLVDNHQIVQANQ
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHCCCEEEEECC
GVLTLQNNNLLDVSIQVPESMAAGLKQYTDQAHFTAKVRFSAFPEQSFDAKFKEYSTQVT
CEEEEECCCEEEEEEECCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHCCCCC
PGTQAYEVVFSLPQPQDIQLLPGMSAELTLALVKDPSALASSIVPMTAIDKRDQDGQVQV
CCCCEEEEEEECCCCCCEEECCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEE
WVYQTQSGEVKPTMVTLGRVSNQGVEILTGINKGDLIVSAGVSQLSAGMKVKPLLLRDNY
EEEECCCCCCCEEEEEEECCCCCCEEEEECCCCCCEEEECCHHHHHCCCEEEEEEEECCC
PDRFSDN
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9037074 [H]