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The map label for this gene is phoA [H]

Identifier: 126175886

GI number: 126175886

Start: 4311200

End: 4312837

Strand: Reverse

Name: phoA [H]

Synonym: Sbal_3691

Alternate gene names: 126175886

Gene position: 4312837-4311200 (Counterclockwise)

Preceding gene: 126175887

Following gene: 126175885

Centisome position: 84.11

GC content: 50.92

Gene sequence:

>1638_bases
ATGAATAAGAAATTACTAGTAGTCGCGATTTCAGCCATGCTGGGTTTAGCCGCATGCGGTAGCGATGGTGATAACGGTAC
CGCGGGAACACCGGGTAGCAATGGATCAAATGGGTCTAACGGTAGCAATGGCAAAGATTGGACTGCTGTGAATCAGTGGT
ATGTCGATGCACAAGCTCGCGTGACCAAAGCCGAAGGCTTAACGGTTAACAATGATGTTGGCGCCGCGAAAAACGTGATC
CTGTTTGTGGGTGACGGTATGGGTGTATCGACCATTACTGCCGCACGTATTTTAGAAGGCCAGCTAAAAGGCAAAACGGG
TGAAGAAAACTCGTTATCTTTCGAAACTCTGCCGTATGTGGGTCTTTCTAAAACCTATAACGTCGACGGCCAAACGCCCG
ATTCTGCAGGCACTATGTCGGCGATGATGACAGGTGTAAAAACTGACGTGGGCGTGATTTCACAAGCTGAAGGCGTAGTA
CGTGCTAACTGTGCTTCGACTAAAGATCAAAATCTTGTGACATCACTTGAGCTTGCCGCAATGGCGGGAATGGCAACGGG
CGTGGTAACGACTGCGCGTTTAACCCACGCAACTCCTGCGGCGACTTACGCCCATGTGCCAGAACGTGATTGGGAAGCCG
ATTCAAACCTACCTGCAGAAGCTGTGACTAACGGCTGTAAAGATATCGCCGCGCAAATGCTCGACTTTAACTACGGCAGC
GGTCTTAACGTGATGATGGGTGGCGGTCGCCGCGCCTTTATTCCTAAGACGACCGTTGATCCAGAAGGTAAAGCCGGTAA
GCGTAACGATGGTCGCGATCTCACTGCTGAGTGGATGAGCAAATACACTAACGCCGCTTACGTGCAAGACAGAGACGGTT
TCCTCAGTGTGGATGCAGCAAGCACAGATCATTTGCTTGGCCTATTCAACTCATCGCACATGGAATACGACTACGACCGT
AAAGAAGCTGGCGTGACTGGTGAGCCATCATTGGCGGAAATGACGGCTAAGTCGATTGATATTCTGAAGAAAAACTCAAA
AGGCTTCGTGCTGATTGTTGAAGCAGGCCGTATCGACCATGCTCACCATGCGGGTAACGCAGCGCGTGCGCTGTATGACA
CAGTGGCATTGTCTGAAGCGGTACGCGTGGCGATGGAAAAAACCTCGGCTAACGATACCTTGTTAATGGTTACAGCGGAT
CACAGCCATGTGTTCACCATCGCAGGTTATCCAACTCGCGGTAACCCTATCCTAGGTCTAGTGAAGTCGAACGACGCCAA
CGGCGTTGCTGAAGTGACTAACTCTACCGATGCTAACGGTTTGCCATATACCACAGTCGGTTACGCTAACGGCATGGGCT
ATGCGTCATTAGCAACAGGTGGCGATGAGCGTTACAACTTCCCAGTGGACGCAGGCCGTAAGGACTTGAACTTTGTTGAT
ACCCAAAGTGCCGGATTCCACCAAGAAGCCCTAGTGCCATTAAGCGATGAGACCCATGCTGGGGAAGACGTAGCGATTTT
CGCCCGCGGTCCAAGTTCGGATCTTATCCAAGGTACGGTTGAGCAGAACCACATTTTCCACGTGATGAACCGCGCGGCAA
ATTTAGTGGTTAAAGCTGAAGCGGCTGCCGTTAAGTAA

Upstream 100 bases:

>100_bases
CAGAACGTGATCTTCCACATTATCAACCAAGCGCAGCAGCTTGGCGGCACTAAGTACTAATCACGTATTAGTTACTGAGT
CCTCACTTTTGGAGCAAAAA

Downstream 100 bases:

>100_bases
GACCCGCTTAGTCTCTGCATAAGTGAAGTCAGAAATAGTGCACTAAGAAATAGTGCACTAAGACATAGTGCAGTAGTAGC
TAAGTAAAAGCAGTGCCGAT

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MNKKLLVVAISAMLGLAACGSDGDNGTAGTPGSNGSNGSNGSNGKDWTAVNQWYVDAQARVTKAEGLTVNNDVGAAKNVI
LFVGDGMGVSTITAARILEGQLKGKTGEENSLSFETLPYVGLSKTYNVDGQTPDSAGTMSAMMTGVKTDVGVISQAEGVV
RANCASTKDQNLVTSLELAAMAGMATGVVTTARLTHATPAATYAHVPERDWEADSNLPAEAVTNGCKDIAAQMLDFNYGS
GLNVMMGGGRRAFIPKTTVDPEGKAGKRNDGRDLTAEWMSKYTNAAYVQDRDGFLSVDAASTDHLLGLFNSSHMEYDYDR
KEAGVTGEPSLAEMTAKSIDILKKNSKGFVLIVEAGRIDHAHHAGNAARALYDTVALSEAVRVAMEKTSANDTLLMVTAD
HSHVFTIAGYPTRGNPILGLVKSNDANGVAEVTNSTDANGLPYTTVGYANGMGYASLATGGDERYNFPVDAGRKDLNFVD
TQSAGFHQEALVPLSDETHAGEDVAIFARGPSSDLIQGTVEQNHIFHVMNRAANLVVKAEAAAVK

Sequences:

>Translated_545_residues
MNKKLLVVAISAMLGLAACGSDGDNGTAGTPGSNGSNGSNGSNGKDWTAVNQWYVDAQARVTKAEGLTVNNDVGAAKNVI
LFVGDGMGVSTITAARILEGQLKGKTGEENSLSFETLPYVGLSKTYNVDGQTPDSAGTMSAMMTGVKTDVGVISQAEGVV
RANCASTKDQNLVTSLELAAMAGMATGVVTTARLTHATPAATYAHVPERDWEADSNLPAEAVTNGCKDIAAQMLDFNYGS
GLNVMMGGGRRAFIPKTTVDPEGKAGKRNDGRDLTAEWMSKYTNAAYVQDRDGFLSVDAASTDHLLGLFNSSHMEYDYDR
KEAGVTGEPSLAEMTAKSIDILKKNSKGFVLIVEAGRIDHAHHAGNAARALYDTVALSEAVRVAMEKTSANDTLLMVTAD
HSHVFTIAGYPTRGNPILGLVKSNDANGVAEVTNSTDANGLPYTTVGYANGMGYASLATGGDERYNFPVDAGRKDLNFVD
TQSAGFHQEALVPLSDETHAGEDVAIFARGPSSDLIQGTVEQNHIFHVMNRAANLVVKAEAAAVK
>Mature_545_residues
MNKKLLVVAISAMLGLAACGSDGDNGTAGTPGSNGSNGSNGSNGKDWTAVNQWYVDAQARVTKAEGLTVNNDVGAAKNVI
LFVGDGMGVSTITAARILEGQLKGKTGEENSLSFETLPYVGLSKTYNVDGQTPDSAGTMSAMMTGVKTDVGVISQAEGVV
RANCASTKDQNLVTSLELAAMAGMATGVVTTARLTHATPAATYAHVPERDWEADSNLPAEAVTNGCKDIAAQMLDFNYGS
GLNVMMGGGRRAFIPKTTVDPEGKAGKRNDGRDLTAEWMSKYTNAAYVQDRDGFLSVDAASTDHLLGLFNSSHMEYDYDR
KEAGVTGEPSLAEMTAKSIDILKKNSKGFVLIVEAGRIDHAHHAGNAARALYDTVALSEAVRVAMEKTSANDTLLMVTAD
HSHVFTIAGYPTRGNPILGLVKSNDANGVAEVTNSTDANGLPYTTVGYANGMGYASLATGGDERYNFPVDAGRKDLNFVD
TQSAGFHQEALVPLSDETHAGEDVAIFARGPSSDLIQGTVEQNHIFHVMNRAANLVVKAEAAAVK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI116734717, Length=491, Percent_Identity=42.7698574338086, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI294660770, Length=463, Percent_Identity=43.4125269978402, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI157266292, Length=467, Percent_Identity=43.254817987152, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI157266296, Length=468, Percent_Identity=42.9487179487179, Blast_Score=367, Evalue=1e-101,
Organism=Homo sapiens, GI94721246, Length=468, Percent_Identity=42.9487179487179, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI294660772, Length=419, Percent_Identity=41.527446300716, Blast_Score=318, Evalue=1e-86,
Organism=Escherichia coli, GI48994877, Length=381, Percent_Identity=34.1207349081365, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320689, Length=344, Percent_Identity=32.5581395348837, Blast_Score=130, Evalue=6e-31,
Organism=Drosophila melanogaster, GI21358067, Length=490, Percent_Identity=42.4489795918367, Blast_Score=348, Evalue=8e-96,
Organism=Drosophila melanogaster, GI21355149, Length=553, Percent_Identity=39.9638336347197, Blast_Score=344, Evalue=9e-95,
Organism=Drosophila melanogaster, GI85725200, Length=471, Percent_Identity=42.2505307855626, Blast_Score=336, Evalue=3e-92,
Organism=Drosophila melanogaster, GI85815833, Length=471, Percent_Identity=42.2505307855626, Blast_Score=336, Evalue=3e-92,
Organism=Drosophila melanogaster, GI19921912, Length=533, Percent_Identity=39.5872420262664, Blast_Score=335, Evalue=7e-92,
Organism=Drosophila melanogaster, GI24651554, Length=491, Percent_Identity=40.9368635437882, Blast_Score=334, Evalue=9e-92,
Organism=Drosophila melanogaster, GI21355151, Length=501, Percent_Identity=39.3213572854291, Blast_Score=324, Evalue=8e-89,
Organism=Drosophila melanogaster, GI24585213, Length=498, Percent_Identity=39.1566265060241, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24651556, Length=465, Percent_Identity=41.505376344086, Blast_Score=314, Evalue=9e-86,
Organism=Drosophila melanogaster, GI21355981, Length=466, Percent_Identity=41.6309012875536, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24657835, Length=484, Percent_Identity=39.2561983471074, Blast_Score=308, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24657842, Length=487, Percent_Identity=38.6036960985626, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24657827, Length=475, Percent_Identity=39.3684210526316, Blast_Score=300, Evalue=1e-81,
Organism=Drosophila melanogaster, GI18859923, Length=492, Percent_Identity=37.6016260162602, Blast_Score=278, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24645593, Length=328, Percent_Identity=29.2682926829268, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 57102; Mature: 57102

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKLLVVAISAMLGLAACGSDGDNGTAGTPGSNGSNGSNGSNGKDWTAVNQWYVDAQAR
CCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCE
VTKAEGLTVNNDVGAAKNVILFVGDGMGVSTITAARILEGQLKGKTGEENSLSFETLPYV
EEECCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
GLSKTYNVDGQTPDSAGTMSAMMTGVKTDVGVISQAEGVVRANCASTKDQNLVTSLELAA
CCCCEEECCCCCCCCCHHHHHHHHCCCCCCCHHHCCCCEEEECCCCCCCCHHHHHHHHHH
MAGMATGVVTTARLTHATPAATYAHVPERDWEADSNLPAEAVTNGCKDIAAQMLDFNYGS
HHHHHHHHEEEEHCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GLNVMMGGGRRAFIPKTTVDPEGKAGKRNDGRDLTAEWMSKYTNAAYVQDRDGFLSVDAA
CCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEEECC
STDHLLGLFNSSHMEYDYDRKEAGVTGEPSLAEMTAKSIDILKKNSKGFVLIVEAGRIDH
CCCCEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHEEECCCCEEEEEECCCCCC
AHHAGNAARALYDTVALSEAVRVAMEKTSANDTLLMVTADHSHVFTIAGYPTRGNPILGL
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEE
VKSNDANGVAEVTNSTDANGLPYTTVGYANGMGYASLATGGDERYNFPVDAGRKDLNFVD
EECCCCCCEEEECCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEE
TQSAGFHQEALVPLSDETHAGEDVAIFARGPSSDLIQGTVEQNHIFHVMNRAANLVVKAE
CCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHCCEEEEEC
AAAVK
CCCCC
>Mature Secondary Structure
MNKKLLVVAISAMLGLAACGSDGDNGTAGTPGSNGSNGSNGSNGKDWTAVNQWYVDAQAR
CCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCE
VTKAEGLTVNNDVGAAKNVILFVGDGMGVSTITAARILEGQLKGKTGEENSLSFETLPYV
EEECCCEEECCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCC
GLSKTYNVDGQTPDSAGTMSAMMTGVKTDVGVISQAEGVVRANCASTKDQNLVTSLELAA
CCCCEEECCCCCCCCCHHHHHHHHCCCCCCCHHHCCCCEEEECCCCCCCCHHHHHHHHHH
MAGMATGVVTTARLTHATPAATYAHVPERDWEADSNLPAEAVTNGCKDIAAQMLDFNYGS
HHHHHHHHEEEEHCCCCCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
GLNVMMGGGRRAFIPKTTVDPEGKAGKRNDGRDLTAEWMSKYTNAAYVQDRDGFLSVDAA
CCEEEECCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCEEEEECCCCEEEEECC
STDHLLGLFNSSHMEYDYDRKEAGVTGEPSLAEMTAKSIDILKKNSKGFVLIVEAGRIDH
CCCCEEEEECCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHEEECCCCEEEEEECCCCCC
AHHAGNAARALYDTVALSEAVRVAMEKTSANDTLLMVTADHSHVFTIAGYPTRGNPILGL
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCCCCCEEEE
VKSNDANGVAEVTNSTDANGLPYTTVGYANGMGYASLATGGDERYNFPVDAGRKDLNFVD
EECCCCCCEEEECCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEE
TQSAGFHQEALVPLSDETHAGEDVAIFARGPSSDLIQGTVEQNHIFHVMNRAANLVVKAE
CCCCCCCCCCEEECCCCCCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHCCEEEEEC
AAAVK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]