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The map label for this gene is cfr9IM [H]

Identifier: 126175812

GI number: 126175812

Start: 4241707

End: 4242597

Strand: Direct

Name: cfr9IM [H]

Synonym: Sbal_3617

Alternate gene names: 126175812

Gene position: 4241707-4242597 (Clockwise)

Preceding gene: 126175811

Following gene: 126175814

Centisome position: 82.73

GC content: 38.95

Gene sequence:

>891_bases
ATGAAGAAGTCTGAATTAGCAAATAAGATATTTACTGGGGACTCATTGGCCTCTCTCAAAGCCCTACCTAAGAACTCTAT
AGACTGTATAGTAACTAGCCCACCGTATTACGGGCAGCGTGATTATGGTATGGATGGACAGATTGGTAACGAAAGCAAGC
TAGAAGAATATATTGAAAACTTAGTAAATATATTTAATGAGTGTAAGCGAGTATTAAAAGACAGTGGAAGCCTTTGGCTA
AATCTTGGAGACAAGTACAACAAAGGAAATCTAATGGGAATGCCTTGGAGAGTTGCTTTAGCGCTTCAGGATGAAGGCTG
GATTTTGAGAAATGACATAATTTGGCATAAGCCTAATGCTATGCCTCATTCAGCTAAAAATCGTTTAACAACAGACCATG
AGTACATATTCTTTTTTACTAAAAAGGCTAAAGACTATTATTACGATCAAGATGCCATTAGAGAAGAGCACAAGACCTTT
TCTGAGGATAGTAAAATGAAGGGAGGTAGAAACCACTTTGGAAAAAATGGTGGGACACCAGAAAAAGGAAAAAACTCTGG
AAACAGTAATTTACATGACGGTAGATGGGATCAGGCATTTCATCCAAATGGAAGAAACAAAAGAACTGTATGGAACGTAC
CTCTTTCAAAATTTAGAGGTGCACACTTCGCTGTTTTTCCAGAAAGACTAATTGAGCCATGCATACTTGCTGGATGCCCA
AAAAACGGAATAGTTTTAGACCCGTTTTTTGGGGCTGGAACAACAGGGTTTGTTGCTGCACAGCAAGGTAGGAAATACGT
AGGCCTTGAGCTAAATCCAGAATATGCTGAAATTGCTGAAAATCGCCTAAAGACCGTACAAACAGAGTTACTCAATCTTT
GCGAAGGTTGA

Upstream 100 bases:

>100_bases
CATATTCTGAAACCACTATACAGGATTTAGTTGTTGAGCTAATAAGACAACGAATTTTACGCGGTGAGTTTTCAAACATG
ATCGAGAGCGAGCAATAGAA

Downstream 100 bases:

>100_bases
AACGAGGGCTTCTAGCTCCTCTTCAAGCTTTGGATTGATCTTATCAAGTTGGTTCGGGTTCTCTTGGATGTAGATATAAT
TTTCACAATTAAGCTCATCA

Product: DNA methylase N-4/N-6 domain-containing protein

Products: NA

Alternate protein names: M.Cfr9I; N(4)- cytosine-specific methyltransferase Cfr9I [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKKSELANKIFTGDSLASLKALPKNSIDCIVTSPPYYGQRDYGMDGQIGNESKLEEYIENLVNIFNECKRVLKDSGSLWL
NLGDKYNKGNLMGMPWRVALALQDEGWILRNDIIWHKPNAMPHSAKNRLTTDHEYIFFFTKKAKDYYYDQDAIREEHKTF
SEDSKMKGGRNHFGKNGGTPEKGKNSGNSNLHDGRWDQAFHPNGRNKRTVWNVPLSKFRGAHFAVFPERLIEPCILAGCP
KNGIVLDPFFGAGTTGFVAAQQGRKYVGLELNPEYAEIAENRLKTVQTELLNLCEG

Sequences:

>Translated_296_residues
MKKSELANKIFTGDSLASLKALPKNSIDCIVTSPPYYGQRDYGMDGQIGNESKLEEYIENLVNIFNECKRVLKDSGSLWL
NLGDKYNKGNLMGMPWRVALALQDEGWILRNDIIWHKPNAMPHSAKNRLTTDHEYIFFFTKKAKDYYYDQDAIREEHKTF
SEDSKMKGGRNHFGKNGGTPEKGKNSGNSNLHDGRWDQAFHPNGRNKRTVWNVPLSKFRGAHFAVFPERLIEPCILAGCP
KNGIVLDPFFGAGTTGFVAAQQGRKYVGLELNPEYAEIAENRLKTVQTELLNLCEG
>Mature_296_residues
MKKSELANKIFTGDSLASLKALPKNSIDCIVTSPPYYGQRDYGMDGQIGNESKLEEYIENLVNIFNECKRVLKDSGSLWL
NLGDKYNKGNLMGMPWRVALALQDEGWILRNDIIWHKPNAMPHSAKNRLTTDHEYIFFFTKKAKDYYYDQDAIREEHKTF
SEDSKMKGGRNHFGKNGGTPEKGKNSGNSNLHDGRWDQAFHPNGRNKRTVWNVPLSKFRGAHFAVFPERLIEPCILAGCP
KNGIVLDPFFGAGTTGFVAAQQGRKYVGLELNPEYAEIAENRLKTVQTELLNLCEG

Specific function: This methylase recognizes the double-stranded sequence CCCGGG, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the Cfr9I endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=283, Percent_Identity=31.095406360424, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 33496; Mature: 33496

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSELANKIFTGDSLASLKALPKNSIDCIVTSPPYYGQRDYGMDGQIGNESKLEEYIEN
CCHHHHHHHHCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
LVNIFNECKRVLKDSGSLWLNLGDKYNKGNLMGMPWRVALALQDEGWILRNDIIWHKPNA
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCEEEEEEEECCCEEEECCEEEECCCC
MPHSAKNRLTTDHEYIFFFTKKAKDYYYDQDAIREEHKTFSEDSKMKGGRNHFGKNGGTP
CCCCHHCCCCCCCEEEEEEEECCCCHHCCHHHHHHHHHHHCCHHHCCCCHHHCCCCCCCC
EKGKNSGNSNLHDGRWDQAFHPNGRNKRTVWNVPLSKFRGAHFAVFPERLIEPCILAGCP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHCCCEEEECHHHHHHHHHCCCCC
KNGIVLDPFFGAGTTGFVAAQQGRKYVGLELNPEYAEIAENRLKTVQTELLNLCEG
CCCEEECCCCCCCCCCCEEHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKSELANKIFTGDSLASLKALPKNSIDCIVTSPPYYGQRDYGMDGQIGNESKLEEYIEN
CCHHHHHHHHCCCCCHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
LVNIFNECKRVLKDSGSLWLNLGDKYNKGNLMGMPWRVALALQDEGWILRNDIIWHKPNA
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCCEEEEEEEECCCEEEECCEEEECCCC
MPHSAKNRLTTDHEYIFFFTKKAKDYYYDQDAIREEHKTFSEDSKMKGGRNHFGKNGGTP
CCCCHHCCCCCCCEEEEEEEECCCCHHCCHHHHHHHHHHHCCHHHCCCCHHHCCCCCCCC
EKGKNSGNSNLHDGRWDQAFHPNGRNKRTVWNVPLSKFRGAHFAVFPERLIEPCILAGCP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHCCCEEEECHHHHHHHHHCCCCC
KNGIVLDPFFGAGTTGFVAAQQGRKYVGLELNPEYAEIAENRLKTVQTELLNLCEG
CCCEEECCCCCCCCCCCEEHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2690010; 8163180 [H]