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The map label for this gene is dusA [H]

Identifier: 126175780

GI number: 126175780

Start: 4210060

End: 4211079

Strand: Reverse

Name: dusA [H]

Synonym: Sbal_3585

Alternate gene names: 126175780

Gene position: 4211079-4210060 (Counterclockwise)

Preceding gene: 126175782

Following gene: 126175779

Centisome position: 82.13

GC content: 47.94

Gene sequence:

>1020_bases
ATGAATTTAGATAAAACCCAGCAACACCAAGGTTTAGCGCCTAAAACCCTCGATAGAACCTTTTCCATTGCGCCCATGCT
GGATTGGACTGATCGCCATTATCGTTATTTTGCGCGTTTGATGTCGGCTAATGCATTGTTGTATACGGAAATGGTGACGA
CTGGGGCGATATTGCATGGTCGTGGTGACTACTTAGCCTATAACCAAGAAGAACATCCTTTGGCGTTGCAGTTAGGTGGC
TCTAATCCTGTCGATTTGGCGCGTTGCGCTAAACTCGCGGCTGAGCGTGGCTACGATGAAGTGAACCTCAATGTGGGTTG
TCCTTCCGATCGAGTGCAGAATGGTCGCTTTGGCGCGTGTTTGATGGCTGAACCTGAATTAGTGGCTGAGTGCGTTGATG
CCATGAAGCAAGTGGTTGATATTCCTGTGACTGTTAAGACACGTATCGGGATTGATGAACAAGACAGTTATGCGTTTTTA
ACCCAGTTTATCGATGTTGTCAGTGCAAAAGGGTGTACGGATTTTACTATTCATGCCCGTAAGGCTTGGCTTCAAGGCTT
GAGTCCGAAAGAGAATCGCGAGATCCCGCCGCTCGATTATGAGCGTGTATATCAACTCAAGCGCGATTATCCCGCGCTCA
ATATTGGTATTAATGGCGGGATCACCACACTTGAGCAAGCGCAACTCCATTTGGCGCAGCTTGATGGCGTGATGATGGGG
CGTGAGGCCTATCAGAATCCTTATATTCTGGCGCAGGTGGACCAAGTTTTATGTGGCAGCCAAAAGGCGGTGATCAGCCG
TGAGGATGTGATTGAGGCAATGTTGCCTTATATCGAAGCACATTTGCTGTCGGGTGGGCGACTTAATCATATTACTCGCC
ATATGATAGGATTATTCCAAGGCTTACCGGGTGCCCGTGCGTGGCGTCGTTATTTGAGCGAAAATGCCCATAAAAACGGC
GCTGGGATAGAAGTAGTAAAGCAGGCTTTTCAGAATATTCAGGCTGAAGTTACAGAGTAA

Upstream 100 bases:

>100_bases
CTCAGTCCAATCCATTGTGGATCACTATAGGTTCCCCGAGCAATGCAAAACATGCAAAAACGTACAAAAATTAGCAAAAA
TATTAACTTTTCGGATTGTA

Downstream 100 bases:

>100_bases
TTCCACTCGACTTAAAAGATGGTGAAATTTACTAATTTGAGTAGTATTTTTCACCACTTGTTATTTTTCAATCCATCCCT
ATATCTCATCTTATCCACAT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNLDKTQQHQGLAPKTLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGG
SNPVDLARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAECVDAMKQVVDIPVTVKTRIGIDEQDSYAFL
TQFIDVVSAKGCTDFTIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPALNIGINGGITTLEQAQLHLAQLDGVMMG
REAYQNPYILAQVDQVLCGSQKAVISREDVIEAMLPYIEAHLLSGGRLNHITRHMIGLFQGLPGARAWRRYLSENAHKNG
AGIEVVKQAFQNIQAEVTE

Sequences:

>Translated_339_residues
MNLDKTQQHQGLAPKTLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGG
SNPVDLARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAECVDAMKQVVDIPVTVKTRIGIDEQDSYAFL
TQFIDVVSAKGCTDFTIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPALNIGINGGITTLEQAQLHLAQLDGVMMG
REAYQNPYILAQVDQVLCGSQKAVISREDVIEAMLPYIEAHLLSGGRLNHITRHMIGLFQGLPGARAWRRYLSENAHKNG
AGIEVVKQAFQNIQAEVTE
>Mature_339_residues
MNLDKTQQHQGLAPKTLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHGRGDYLAYNQEEHPLALQLGG
SNPVDLARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGACLMAEPELVAECVDAMKQVVDIPVTVKTRIGIDEQDSYAFL
TQFIDVVSAKGCTDFTIHARKAWLQGLSPKENREIPPLDYERVYQLKRDYPALNIGINGGITTLEQAQLHLAQLDGVMMG
REAYQNPYILAQVDQVLCGSQKAVISREDVIEAMLPYIEAHLLSGGRLNHITRHMIGLFQGLPGARAWRRYLSENAHKNG
AGIEVVKQAFQNIQAEVTE

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=302, Percent_Identity=25.1655629139073, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI239788483, Length=267, Percent_Identity=25.0936329588015, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI239788462, Length=238, Percent_Identity=25.2100840336134, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI145693211, Length=312, Percent_Identity=70.8333333333333, Blast_Score=464, Evalue=1e-132,
Organism=Escherichia coli, GI1789660, Length=239, Percent_Identity=25.9414225941423, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788462, Length=178, Percent_Identity=31.4606741573034, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=170, Percent_Identity=30, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=235, Percent_Identity=26.8085106382979, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323560, Length=237, Percent_Identity=24.8945147679325, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24580595, Length=242, Percent_Identity=26.8595041322314, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920448, Length=242, Percent_Identity=26.8595041322314, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37828; Mature: 37828

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDKTQQHQGLAPKTLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHG
CCCCCCHHHCCCCCHHHHCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCEEEEC
RGDYLAYNQEEHPLALQLGGSNPVDLARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGAC
CCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEE
LMAEPELVAECVDAMKQVVDIPVTVKTRIGIDEQDSYAFLTQFIDVVSAKGCTDFTIHAR
EECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHH
KAWLQGLSPKENREIPPLDYERVYQLKRDYPALNIGINGGITTLEQAQLHLAQLDGVMMG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
REAYQNPYILAQVDQVLCGSQKAVISREDVIEAMLPYIEAHLLSGGRLNHITRHMIGLFQ
HHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GLPGARAWRRYLSENAHKNGAGIEVVKQAFQNIQAEVTE
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLDKTQQHQGLAPKTLDRTFSIAPMLDWTDRHYRYFARLMSANALLYTEMVTTGAILHG
CCCCCCHHHCCCCCHHHHCCEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCEEEEC
RGDYLAYNQEEHPLALQLGGSNPVDLARCAKLAAERGYDEVNLNVGCPSDRVQNGRFGAC
CCCEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCCEE
LMAEPELVAECVDAMKQVVDIPVTVKTRIGIDEQDSYAFLTQFIDVVSAKGCTDFTIHAR
EECCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEHHHH
KAWLQGLSPKENREIPPLDYERVYQLKRDYPALNIGINGGITTLEQAQLHLAQLDGVMMG
HHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHH
REAYQNPYILAQVDQVLCGSQKAVISREDVIEAMLPYIEAHLLSGGRLNHITRHMIGLFQ
HHHHCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
GLPGARAWRRYLSENAHKNGAGIEVVKQAFQNIQAEVTE
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]