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The map label for this gene is nudF [H]

Identifier: 126175773

GI number: 126175773

Start: 4203856

End: 4204458

Strand: Reverse

Name: nudF [H]

Synonym: Sbal_3577

Alternate gene names: 126175773

Gene position: 4204458-4203856 (Counterclockwise)

Preceding gene: 126175775

Following gene: 126175772

Centisome position: 82.0

GC content: 45.94

Gene sequence:

>603_bases
ATGAAACCCCCTGTTTTTTCGCAAACGGATATTGAGATCTTAGAAAAAAAATCCCTATACCAAGGATTTTTCTCAATGGA
CGAATACACTTTCAAACATAAATTATTTGCTGGCGGCTGGAGCAAACCTGTCACTCGCGAAGTGTTTGAACGGGGTCATG
CCGTGGTCGTTCTGCCCTATGATGCCAGACAAGATAAAGTAGTACTCATCGAACAGGTGCGTTTTCCTGCATTAGCAACC
ACTAAATCTCCTTGGCTTCTCGAGTTGGTCGCGGGTATGATTGCGCCCGAAGAAACGCCTCTGGACGTTGCGCACCGCGA
ATTAATGGAAGAAACCGGCTTAGTTGCTAAGCAAATGCACAGCGTGAATAGTTATCTTGCCAGCCCAGGTGGTAGCACAG
AACGTTTCTATTTCTATTGGGCCGATGTCGATTCGAACGACGCCCGTGGATTGCATGGATTAGCTGAAGAGCACGAAGAT
ATTCGATTGCATGTCATGAGCCGCGAGCAAGCTTATAATAGTGTGGTTGCGGGCGAGATCGATAATGCGTCGACCGTCAT
TGGATTACAATGGTTACAATTAAACTATCAACAACTGGTTTAA

Upstream 100 bases:

>100_bases
GACACTTTCAAGTCAAATTCGAAGTGTAACAGATTTTATTTTAAAAATGTGCTCAAATTGCCTTGATTATAAGCGCAAAT
TCAACTTTTAAGGATAGAGT

Downstream 100 bases:

>100_bases
GTTGGGATGAATTTGGCTACTTCGAGTCTAAAACAGCGCTATAAACCTAACGTCAGTGACTTTTTAGCACTGTGCGGGCG
AAATTATGGTTACATGTTGA

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MKPPVFSQTDIEILEKKSLYQGFFSMDEYTFKHKLFAGGWSKPVTREVFERGHAVVVLPYDARQDKVVLIEQVRFPALAT
TKSPWLLELVAGMIAPEETPLDVAHRELMEETGLVAKQMHSVNSYLASPGGSTERFYFYWADVDSNDARGLHGLAEEHED
IRLHVMSREQAYNSVVAGEIDNASTVIGLQWLQLNYQQLV

Sequences:

>Translated_200_residues
MKPPVFSQTDIEILEKKSLYQGFFSMDEYTFKHKLFAGGWSKPVTREVFERGHAVVVLPYDARQDKVVLIEQVRFPALAT
TKSPWLLELVAGMIAPEETPLDVAHRELMEETGLVAKQMHSVNSYLASPGGSTERFYFYWADVDSNDARGLHGLAEEHED
IRLHVMSREQAYNSVVAGEIDNASTVIGLQWLQLNYQQLV
>Mature_200_residues
MKPPVFSQTDIEILEKKSLYQGFFSMDEYTFKHKLFAGGWSKPVTREVFERGHAVVVLPYDARQDKVVLIEQVRFPALAT
TKSPWLLELVAGMIAPEETPLDVAHRELMEETGLVAKQMHSVNSYLASPGGSTERFYFYWADVDSNDARGLHGLAEEHED
IRLHVMSREQAYNSVVAGEIDNASTVIGLQWLQLNYQQLV

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=197, Percent_Identity=55.8375634517767, Blast_Score=232, Evalue=1e-62,
Organism=Escherichia coli, GI1788810, Length=187, Percent_Identity=29.9465240641711, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 22703; Mature: 22703

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPPVFSQTDIEILEKKSLYQGFFSMDEYTFKHKLFAGGWSKPVTREVFERGHAVVVLPY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCEEEEEEC
DARQDKVVLIEQVRFPALATTKSPWLLELVAGMIAPEETPLDVAHRELMEETGLVAKQMH
CCCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
SVNSYLASPGGSTERFYFYWADVDSNDARGLHGLAEEHEDIRLHVMSREQAYNSVVAGEI
HHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHCCCCEEEEEEEHHHHHHHHHCCCC
DNASTVIGLQWLQLNYQQLV
CCCCEEEEHHHEEHHHHHCC
>Mature Secondary Structure
MKPPVFSQTDIEILEKKSLYQGFFSMDEYTFKHKLFAGGWSKPVTREVFERGHAVVVLPY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHCCCEEEEEEC
DARQDKVVLIEQVRFPALATTKSPWLLELVAGMIAPEETPLDVAHRELMEETGLVAKQMH
CCCCCCEEEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
SVNSYLASPGGSTERFYFYWADVDSNDARGLHGLAEEHEDIRLHVMSREQAYNSVVAGEI
HHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCCHHHCCCCEEEEEEEHHHHHHHHHCCCC
DNASTVIGLQWLQLNYQQLV
CCCCEEEEHHHEEHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]