Click here to switch to the map view.

The map label for this gene is cysH [H]

Identifier: 126175611

GI number: 126175611

Start: 4000931

End: 4001713

Strand: Reverse

Name: cysH [H]

Synonym: Sbal_3414

Alternate gene names: 126175611

Gene position: 4001713-4000931 (Counterclockwise)

Preceding gene: 126175612

Following gene: 126175610

Centisome position: 78.05

GC content: 47.77

Gene sequence:

>783_bases
ATGGTTGATCATCAACAAAAAACCAGTGATACAGTGCCGACTGTGGTCAGCACGGCAGAGTTAACGGCGCTATTGAGTGC
GCCCAAGGCCGAGCAAGCGATAGAGCTTGAACGCATAAATCAGTTCTTGGCCGGATTGAGCGCACAAGAGCGAGTGTTAT
GGGGCTTAACTTATTTGCCCGGTAATCATGCTTTGTCGTCAAGCTTTGGTATTCAAGCCGCTGTAATGCTGCATATGGTG
AGTCAGGCACAGGCGAATATTCCGGTTATCCTAACGGATACCGGTTATTTGTTCCCAGAGACCTATCTGTTCATCGATGA
ACTGACACAGCGTTTGTCACTGAATGTAAAAGTGTATCAGGCGCCGATGACTTCGGCTTGGCAAGAGGCGCGCTTTGGTA
AGTTGTGGGAACAAGGGTTAGACGGATTAGAGCAATATAACCGTTTAAACAAAGTTGAGCCCATGCAACGTGCTTTGGCT
GAGCTTGAAGTCGGCACTTGGTTTGCGGGACTGAGACGTAGCCAAGCTAGCACCCGTGAGGCGCTGCCGATATTGGCGAT
TCATGGTACTCGCTTTAAGTTACTGCCAATTATCGAATGGTCGAATAAAGATGTGCACCTATATTTGACTCAATTTGACT
TGCCGTACCACCCACTTTGGGACCAAGGCTATGTTTCTGTTGGTGATACCCATTCGAGTAAACCACTTGAATTAGGGATG
ACAGAGGAAGAAACGCGCTTTAATGGGCTGAAACGTGAGTGTGGTCTGCATTACGATATCTAG

Upstream 100 bases:

>100_bases
GGCCGCTACGCCGTCGAGCGTGATGCAGGCGAAACCTTTGGTAACTTCACCGTCCGCGTGGGTGTTGTGAAGGCGGTGAT
TGATGCCGCTAAGGATTTTC

Downstream 100 bases:

>100_bases
TCATCTTATTGATTTAAAATATTAAACGCCTCAATTGCCATTGGGGCGTTTTATATCTCTGTAAGATAAGTGTTATCTTT
GGTTGAATTTAATACTCTGG

Product: phosphoadenosine phosphosulfate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MVDHQQKTSDTVPTVVSTAELTALLSAPKAEQAIELERINQFLAGLSAQERVLWGLTYLPGNHALSSSFGIQAAVMLHMV
SQAQANIPVILTDTGYLFPETYLFIDELTQRLSLNVKVYQAPMTSAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALA
ELEVGTWFAGLRRSQASTREALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
TEEETRFNGLKRECGLHYDI

Sequences:

>Translated_260_residues
MVDHQQKTSDTVPTVVSTAELTALLSAPKAEQAIELERINQFLAGLSAQERVLWGLTYLPGNHALSSSFGIQAAVMLHMV
SQAQANIPVILTDTGYLFPETYLFIDELTQRLSLNVKVYQAPMTSAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALA
ELEVGTWFAGLRRSQASTREALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
TEEETRFNGLKRECGLHYDI
>Mature_260_residues
MVDHQQKTSDTVPTVVSTAELTALLSAPKAEQAIELERINQFLAGLSAQERVLWGLTYLPGNHALSSSFGIQAAVMLHMV
SQAQANIPVILTDTGYLFPETYLFIDELTQRLSLNVKVYQAPMTSAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALA
ELEVGTWFAGLRRSQASTREALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
TEEETRFNGLKRECGLHYDI

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=242, Percent_Identity=61.1570247933884, Blast_Score=314, Evalue=4e-87,
Organism=Saccharomyces cerevisiae, GI6325425, Length=243, Percent_Identity=32.0987654320988, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR011800
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 29358; Mature: 29358

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDHQQKTSDTVPTVVSTAELTALLSAPKAEQAIELERINQFLAGLSAQERVLWGLTYLP
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEEECC
GNHALSSSFGIQAAVMLHMVSQAQANIPVILTDTGYLFPETYLFIDELTQRLSLNVKVYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
APMTSAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALAELEVGTWFAGLRRSQASTRE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
CCEEEEECCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCEEECC
TEEETRFNGLKRECGLHYDI
CCHHHHHHCHHHHCCCCCCC
>Mature Secondary Structure
MVDHQQKTSDTVPTVVSTAELTALLSAPKAEQAIELERINQFLAGLSAQERVLWGLTYLP
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHEEEEEEECC
GNHALSSSFGIQAAVMLHMVSQAQANIPVILTDTGYLFPETYLFIDELTQRLSLNVKVYQ
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
APMTSAWQEARFGKLWEQGLDGLEQYNRLNKVEPMQRALAELEVGTWFAGLRRSQASTRE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPILAIHGTRFKLLPIIEWSNKDVHLYLTQFDLPYHPLWDQGYVSVGDTHSSKPLELGM
CCEEEEECCCCEEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCEEECC
TEEETRFNGLKRECGLHYDI
CCHHHHHHCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]