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The map label for this gene is cobS

Identifier: 126175560

GI number: 126175560

Start: 3928917

End: 3929705

Strand: Reverse

Name: cobS

Synonym: Sbal_3363

Alternate gene names: 126175560

Gene position: 3929705-3928917 (Counterclockwise)

Preceding gene: 126175561

Following gene: 126175559

Centisome position: 76.64

GC content: 51.46

Gene sequence:

>789_bases
ATGTCAGAACGCGAAAGCTGGCACAAAGAGATAGATTTGTTTTTAGTTGCCATGGGCTATTTTACCCGCATTCCTATGCC
TAAATGGGTCGAAGCCGATAGCGATAAGTTGAATAAGGCCAGCCGTTATTTCGGTCTCGTCGGTCTTTTGGTCGGCCTGT
TAAGTGCCATTGTATTTTGGCTGACGCAGAACTGGCTCCCCGCCGGCGTCTCTGTGCTGTTAGCTATGTTGGTCGGCGTC
TTGCTCACGGGCGGATTCCACGAGGATGGCCTAGCCGATACCTTCGACGGTTTTGGCGGTGGTTGGACGGCGGAAGACAA
GCTACGGATCATGAAGGACTCGCGCCTTGGCAGTTATGGCGCACTGGCGTTGATGTTGGCCCTGCTGCTGAAATGGCAGT
TGTTAGTCGAGTTGGCGCTGTACGATCCTGTGGTCGCGGGCTCGGCGCTTATCGTCGCGCACACAGTCAGCCGCATGGTA
TCAGCCAGTATCATCTTTAGTGAAAAATATGTCCGCGATGACGAGACCAGTAAGTCTAAGCCGCTATCGCAACATCAAGG
CATTAATGAGCTGCTTATCCTGATCGCCAGCGGCGTATTAGTGTTGTTATTCCTCAAGGGGTTAGCGGCATTGTCATTGC
TATTGGTGATGATAGGACTGCGCCGTTTGATCATAGTGATCTTTAGACGCCAGATTGGCGGTTACACGGGTGATACCTTA
GGCGCGGCGCAGCAAATAGCTGAAATCGTGTGTTATTTTGTATTGCTCGTTGTGGGTAACATACTGTGA

Upstream 100 bases:

>100_bases
CTGCTAAGTCGCTTATTTTACTTATTTAAAATAATAACTTTTTAGCCAACCGTTTTAATCTTAAACCTTGCAGCAAGTGT
TAATTCACTAAGGATGCTTT

Downstream 100 bases:

>100_bases
TCCACCTCGTCCTAGGCGGCGCCCGTAGCGGTAAGAGTCGCTACGGTGAAGCCTTAGTGCGCCAGTACACTGCGCTCGGC
TTCGATGCCTGCTATGTTGC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEADSDKLNKASRYFGLVGLLVGLLSAIVFWLTQNWLPAGVSVLLAMLVGV
LLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGALALMLALLLKWQLLVELALYDPVVAGSALIVAHTVSRMV
SASIIFSEKYVRDDETSKSKPLSQHQGINELLILIASGVLVLLFLKGLAALSLLLVMIGLRRLIIVIFRRQIGGYTGDTL
GAAQQIAEIVCYFVLLVVGNIL

Sequences:

>Translated_262_residues
MSERESWHKEIDLFLVAMGYFTRIPMPKWVEADSDKLNKASRYFGLVGLLVGLLSAIVFWLTQNWLPAGVSVLLAMLVGV
LLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGALALMLALLLKWQLLVELALYDPVVAGSALIVAHTVSRMV
SASIIFSEKYVRDDETSKSKPLSQHQGINELLILIASGVLVLLFLKGLAALSLLLVMIGLRRLIIVIFRRQIGGYTGDTL
GAAQQIAEIVCYFVLLVVGNIL
>Mature_261_residues
SERESWHKEIDLFLVAMGYFTRIPMPKWVEADSDKLNKASRYFGLVGLLVGLLSAIVFWLTQNWLPAGVSVLLAMLVGVL
LTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYGALALMLALLLKWQLLVELALYDPVVAGSALIVAHTVSRMVS
ASIIFSEKYVRDDETSKSKPLSQHQGINELLILIASGVLVLLFLKGLAALSLLLVMIGLRRLIIVIFRRQIGGYTGDTLG
AAQQIAEIVCYFVLLVVGNIL

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=35.2226720647773, Blast_Score=130, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_SHEB5 (A3D7X7)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001051709.1
- STRING:   A3D7X7
- GeneID:   4842202
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_3363
- NMPDR:   fig|325240.3.peg.3497
- eggNOG:   COG0368
- HOGENOM:   HBG729392
- OMA:   HDSRIGA
- PhylomeDB:   A3D7X7
- ProtClustDB:   PRK00235
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 28681; Mature: 28550

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x171d89a8)-; HASH(0x18978cb4)-; HASH(0x18b2e6a4)-; HASH(0x1880a460)-; HASH(0x18a7f08c)-; HASH(0x1875d1ac)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
SERESWHKEIDLFLVAMGYFTRIPMPKWVEADSDKLNKASRYFGLVGLLVGLLSAIVFW
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTQNWLPAGVSVLLAMLVGVLLTGGFHEDGLADTFDGFGGGWTAEDKLRIMKDSRLGSYG
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCHHHHHHHHHCCCCCHH
ALALMLALLLKWQLLVELALYDPVVAGSALIVAHTVSRMVSASIIFSEKYVRDDETSKSK
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLSQHQGINELLILIASGVLVLLFLKGLAALSLLLVMIGLRRLIIVIFRRQIGGYTGDTL
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GAAQQIAEIVCYFVLLVVGNIL
HHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA