Click here to switch to the map view.

The map label for this gene is cobQ [H]

Identifier: 126175558

GI number: 126175558

Start: 3926548

End: 3928230

Strand: Reverse

Name: cobQ [H]

Synonym: Sbal_3361

Alternate gene names: 126175558

Gene position: 3928230-3926548 (Counterclockwise)

Preceding gene: 126175559

Following gene: 126175557

Centisome position: 76.61

GC content: 50.45

Gene sequence:

>1683_bases
ATGTCCTCTCCAAACACAGACAACACCGACTGTGCAACTGCACTCTATACCGCTAACACTGTTGCTATTCATCCTGCGGC
TAATCTTGCAGTGAATACGCCAGCGGTTAATTCTGCGGTGAATAGGTCTGCTGTTAATTCACCTCGGCGTACAAAAGTCT
TGATGGTGCAGGGCACAACGTCGGATGCGGGTAAAAGCACCTTAGTGGCAGGCATTTGTCGTTTGCTCGCCCGAGAGGGC
GTAAAGGTTGCGCCCTTTAAGCCGCAAAATATGGCGCTCAATAGCGCTGTAACTATTGATGGCGGCGAGATCGGCCGCGC
ACAGGCGTTGCAAGCCGTGGCCTGTTATCTTGAGCCCCATACCGATTTTAATCCTATTCTGCTTAAACCTAGCTCGGACA
CGGGCGCGCAAATTATCGTTCACGGCAAAGCGCTGACGACGCTCGAAGCCTCGGCCTTTTTTGGGCCTAAATCTAAGGAT
TACAAGGCAATGGCGCTCGGTGCTGTGCTGGAATCCTTCGAGCGATTAAGTGGCCAGTATCAAACCATAGTGGTGGAAGG
CGCGGGGAGTCCGGCGGAGATCAATCTGCGCGAGGGCGATATTGCCAACATGGGATTTGCCGAAGCTGTAGATTGCCCAG
TGATTATTATTGCCGATATCGATAAAGGTGGCGTATTTGCCCATTTGGTCGGGACGCTGGCGCTGTTATCTGAGTCTGAA
CAGGCGCGGGTAAAGGGCTTTGTGATCAATCGTTTCCGTGGCGATATTAGCCTGTTGCAATCTGGCCTCGATTGGCTTGA
AGCTTATACGAACAAGCCTGTTTTAGGCGTGTTGCCTTATCTGCATGATTTGCATCTCGATGCCGAAGATGCGCTGATAA
GCTCGCCGACTAAAGGCGCTAACAGCCGCTTTAAAGTGCGGGTATTAGTGTACCCAAGGATCAGTAATCACACGGATTTT
GATCCGCTGCGGTTGCATCCCGATATCGATTTTGACTATGTGTCATTGCAAACCCAAGCCGCGAGTCACGCCACTCATTT
GCCCGCCGCCGATTTATTGATCTTGCCCGGCAGTAAGAATGTGCGTGCCGATTTGGCCTTTTTACGCCAGCAAGGTTGGG
ATAAGGATATCGACACACACCTGCGTTACGGCGGCAAAGTGCTCGGCATTTGCGGCGGTTACCAAATGTTAGGCCAAAGC
ATTGCCGATCCCTTAGGGATTGAAGACAGTGCGGGCGATACCGAAGGACTTGGCTACTTACCATTGAGCACAGAACTGAA
AGCCGATAAACAGTTACGGCGGGTGAGCGGTGAGCTAACGTTACTTGGGCAAAAGGTTGCAGTGAAGGGCTATGAAATCC
ACTGCGGCGAATCACGTTATCTTATCCCTAGGCTTGAACAAATGAATGCGCCCTTGCAGTTAATCCCTGAATCCACATCT
GTGAGCTTTGCTGATGGCCTGCAAAGCGATGACGGGCAAATCCTTGGCACTTATTTACATGGGCTGTTTGATAGCCCTGA
TACCTGTCAGTTGATTTTACGTTGGGCGGGATTAAACGATGCTAAGACCATAGATATCAATCAAATACGTGAGCAGCAAC
TCGATCGTTTAGCCGATGTGCTAGTCGAGCATCTAGACATGCAAAAATTACAGACTATTTTGGATGCTGCAGTTCAGCGT
TGA

Upstream 100 bases:

>100_bases
GGGTTTACCCTTGGCACTCAAACCACTGTAATCTAACTAAGTGATGTTGAGACAGAGCTTTATCTTTATCCCTGCAAACT
AGAATAAGTTTTTTAAACCT

Downstream 100 bases:

>100_bases
ATTCGGCAAAGAACGGATATTGTGAGAGCGGCATTTAGAAATTAACCATTTGCCGCAGTCTATTGAACCATTTATAAAAG
CATACATTTTTAGCAGCAGA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MSSPNTDNTDCATALYTANTVAIHPAANLAVNTPAVNSAVNRSAVNSPRRTKVLMVQGTTSDAGKSTLVAGICRLLAREG
VKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGPKSKD
YKAMALGAVLESFERLSGQYQTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESE
QARVKGFVINRFRGDISLLQSGLDWLEAYTNKPVLGVLPYLHDLHLDAEDALISSPTKGANSRFKVRVLVYPRISNHTDF
DPLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKNVRADLAFLRQQGWDKDIDTHLRYGGKVLGICGGYQMLGQS
IADPLGIEDSAGDTEGLGYLPLSTELKADKQLRRVSGELTLLGQKVAVKGYEIHCGESRYLIPRLEQMNAPLQLIPESTS
VSFADGLQSDDGQILGTYLHGLFDSPDTCQLILRWAGLNDAKTIDINQIREQQLDRLADVLVEHLDMQKLQTILDAAVQR

Sequences:

>Translated_560_residues
MSSPNTDNTDCATALYTANTVAIHPAANLAVNTPAVNSAVNRSAVNSPRRTKVLMVQGTTSDAGKSTLVAGICRLLAREG
VKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGPKSKD
YKAMALGAVLESFERLSGQYQTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESE
QARVKGFVINRFRGDISLLQSGLDWLEAYTNKPVLGVLPYLHDLHLDAEDALISSPTKGANSRFKVRVLVYPRISNHTDF
DPLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKNVRADLAFLRQQGWDKDIDTHLRYGGKVLGICGGYQMLGQS
IADPLGIEDSAGDTEGLGYLPLSTELKADKQLRRVSGELTLLGQKVAVKGYEIHCGESRYLIPRLEQMNAPLQLIPESTS
VSFADGLQSDDGQILGTYLHGLFDSPDTCQLILRWAGLNDAKTIDINQIREQQLDRLADVLVEHLDMQKLQTILDAAVQR
>Mature_559_residues
SSPNTDNTDCATALYTANTVAIHPAANLAVNTPAVNSAVNRSAVNSPRRTKVLMVQGTTSDAGKSTLVAGICRLLAREGV
KVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPHTDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGPKSKDY
KAMALGAVLESFERLSGQYQTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESEQ
ARVKGFVINRFRGDISLLQSGLDWLEAYTNKPVLGVLPYLHDLHLDAEDALISSPTKGANSRFKVRVLVYPRISNHTDFD
PLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKNVRADLAFLRQQGWDKDIDTHLRYGGKVLGICGGYQMLGQSI
ADPLGIEDSAGDTEGLGYLPLSTELKADKQLRRVSGELTLLGQKVAVKGYEIHCGESRYLIPRLEQMNAPLQLIPESTSV
SFADGLQSDDGQILGTYLHGLFDSPDTCQLILRWAGLNDAKTIDINQIREQQLDRLADVLVEHLDMQKLQTILDAAVQR

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 60378; Mature: 60247

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPNTDNTDCATALYTANTVAIHPAANLAVNTPAVNSAVNRSAVNSPRRTKVLMVQGTT
CCCCCCCCCCHHEEEEECCEEEECCCCCEEECCCCHHHHHHHHHCCCCCCEEEEEEECCC
SDAGKSTLVAGICRLLAREGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPH
CCCCHHHHHHHHHHHHHHCCCEECCCCCCCEEECCEEEECCCCCCHHHHHHHHHEEECCC
TDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGPKSKDYKAMALGAVLESFERLSGQY
CCCCCEEEECCCCCCCEEEEECCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCE
QTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESE
EEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
QARVKGFVINRFRGDISLLQSGLDWLEAYTNKPVLGVLPYLHDLHLDAEDALISSPTKGA
HHHHHEEEEEHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCC
NSRFKVRVLVYPRISNHTDFDPLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKN
CCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEHHHHHHCCCCCCCCEEEECCCCC
VRADLAFLRQQGWDKDIDTHLRYGGKVLGICGGYQMLGQSIADPLGIEDSAGDTEGLGYL
CHHHHHHHHHCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEEEE
PLSTELKADKQLRRVSGELTLLGQKVAVKGYEIHCGESRYLIPRLEQMNAPLQLIPESTS
CCCCCHHHHHHHHHHCCCEEEEECEEEEEEEEEEECCCCEECCCHHHCCCCEEEECCCCC
VSFADGLQSDDGQILGTYLHGLFDSPDTCQLILRWAGLNDAKTIDINQIREQQLDRLADV
EEHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHEEECCCCCCCEEEHHHHHHHHHHHHHHH
LVEHLDMQKLQTILDAAVQR
HHHHHCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSPNTDNTDCATALYTANTVAIHPAANLAVNTPAVNSAVNRSAVNSPRRTKVLMVQGTT
CCCCCCCCCHHEEEEECCEEEECCCCCEEECCCCHHHHHHHHHCCCCCCEEEEEEECCC
SDAGKSTLVAGICRLLAREGVKVAPFKPQNMALNSAVTIDGGEIGRAQALQAVACYLEPH
CCCCHHHHHHHHHHHHHHCCCEECCCCCCCEEECCEEEECCCCCCHHHHHHHHHEEECCC
TDFNPILLKPSSDTGAQIIVHGKALTTLEASAFFGPKSKDYKAMALGAVLESFERLSGQY
CCCCCEEEECCCCCCCEEEEECCEEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCE
QTIVVEGAGSPAEINLREGDIANMGFAEAVDCPVIIIADIDKGGVFAHLVGTLALLSESE
EEEEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
QARVKGFVINRFRGDISLLQSGLDWLEAYTNKPVLGVLPYLHDLHLDAEDALISSPTKGA
HHHHHEEEEEHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCC
NSRFKVRVLVYPRISNHTDFDPLRLHPDIDFDYVSLQTQAASHATHLPAADLLILPGSKN
CCEEEEEEEEEECCCCCCCCCCEEECCCCCCEEEEEEHHHHHHCCCCCCCCEEEECCCCC
VRADLAFLRQQGWDKDIDTHLRYGGKVLGICGGYQMLGQSIADPLGIEDSAGDTEGLGYL
CHHHHHHHHHCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCEEEE
PLSTELKADKQLRRVSGELTLLGQKVAVKGYEIHCGESRYLIPRLEQMNAPLQLIPESTS
CCCCCHHHHHHHHHHCCCEEEEECEEEEEEEEEEECCCCEECCCHHHCCCCEEEECCCCC
VSFADGLQSDDGQILGTYLHGLFDSPDTCQLILRWAGLNDAKTIDINQIREQQLDRLADV
EEHHCCCCCCCCCCHHHHHHHHCCCCCHHHHHEEECCCCCCCEEEHHHHHHHHHHHHHHH
LVEHLDMQKLQTILDAAVQR
HHHHHCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]