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The map label for this gene is pnp [H]

Identifier: 126175434

GI number: 126175434

Start: 3790508

End: 3792619

Strand: Reverse

Name: pnp [H]

Synonym: Sbal_3234

Alternate gene names: 126175434

Gene position: 3792619-3790508 (Counterclockwise)

Preceding gene: 126175436

Following gene: 126175433

Centisome position: 73.97

GC content: 45.55

Gene sequence:

>2112_bases
ATGGGTCACGTGAATCCTATTGTAAAGAGTTTTGAGTATGGTCAACATACAGTCACCCTGGAAACAGGTGTTATAGCGCG
TCAAGCAGATGCAGCCGTTTTAGCGAGTATGGGTGACACGACTGTGTTGGTCACTGTTGTCGGTAAAAAAGAAGCAGATG
CTGGTCGTGACTTTTTCCCTCTGACTGTTAACTATCAAGAAAAAACCTACGCAGCAGGTAAAATTCCTGGTGGTTTCTTT
AAGCGTGAAGGCCGTCCTTCAGAAGATGAGACATTAATCGCTCGTCTGATCGACCGTCCAATTCGCCCTCTTTTCCCTAA
TGGTTTCAAAAACGAAGTTCAAGTTATCATTACTGTAGTATCTGTCGATCCACAAATCGAGCCAGATATCATCTCTATGA
TCGGTACTTCTGCGGCACTGGCTATTTCTGGTATTCCATTCAGCGGTCCTTTAGGTGCTGCACGTGTGGGTTACATTGAT
GGCGAATACGTGTTGAACCCAAGTGTTGCGCAACTTGCAACCAGCCAACTGAACTTAGTGGTTGCTGGTACTGCTGGTGC
AGTATTAATGGTGGAATCAGAAGCCCAAGCATTACCAGAAGAAGTGATGTTAGGTTCTGTTGTTTACGGTCACGATCAAC
AACAAGTGGTTATCAAAGCGATTGCTGAATTTAAAGCAGAAGCCGGTAAACCAACTTGGGATTGGACTGCACCAACGCAA
GATGCCGATTTAGTTGCCCAAATCAAAGAGCTTGCTGAAGCTGGTTTAGGTGATGCGTATAAGATCCAAGTTAAACAAGA
TCGTTATGCTCAAGTTTCTGTTGTTAAAGCAGCAACAAAAGAAGCCTTGTTAGCTAGCAATCCAAGCATTGATTTACGTG
AAGTAGACAACTTGCTGGGTAGCTTAGAGAAGAAAGTGGTTCGTGGTCGCATCATCCGTGGTGAGCCACGTATTGATGGT
CGTGAACCAGACATGATCCGTGCATTAAGCGTGTTAGCGGGCGTATTGCCACGTACTCACGGTAGCGCATTGTTCACTCG
CGGTGAAACTCAAGCGTTAGTGACTTGTACTTTAGGCACTGAACGTGATGCACAGAAGATTGACAGCATCATGGGCGAGC
GCACTAACCGTTTCATGCTGCACTATAACTTCCCTCCATACTCTGTTGGTGAAACTGGCATGGTTGGTTCACCTAAGCGC
CGCGAAATCGGTCATGGTAAGTTAGCATGGCGCGGTATCAATGCTGTTATGCCTTCAGCTGCTGAATTCCCATACAGCGT
GCGCGTTGTATCTGAAATCACTGAATCTAACGGTTCAAGCTCTATGGCGTCAGTATGTGGTACTTCACTAGCGCTGATGG
ATGCTGGTGTGCCTATCAAAACTTCTGTAGCGGGTATCGCTATGGGTCTAGTGAAAGAAGGCGATGATTTCGTTGTTCTT
TCTGACATCTTAGGTGATGAAGATCATTTAGGTGACATGGACTTTAAAGTTGCGGGTACGCGTGATGGTATTACTGCACT
GCAGATGGATATCAAGATCGAAGGTATCACTAAAGAAATCATGGATATCGCACTGCAACAAGCCTATGGCGCACGTGTAC
ATATCCTGAACGTGATGGATCAAGCGATTGGTTCACACCGTGATGATATTTCTGACCACGCTCCACGTATCACTGTTATC
AAGATCAACCCAGAAAAAATTCGTGACGTTATCGGTAAAGGCGGCGCAGTTATCCGTGCTCTTACTGAAGAAACCGGCAC
TACGATTGAACTGGAAGATGACGGTACTGTGAAGATCGCTTCTTCAAACGGCGAAGCAACCAAAGAAGCTATCCGTCGTA
TCGAAGAAATCACTTCAGAAGTTGAAGTGGGTCGTATCTACAACGGTAAAGTTATCCGTATCGTTGATTTCGGTGCGTTC
GTGAACATTCTGCCGGGCAAAGACGGTTTAGTACACATTTCACAAATCAGTGATGAGCGTGTTGCTAACGTGTCTGATCA
CCTTGAACTGAACCAAGAAGTTGCTGTTAAAGTAATGGAAGTGGATCGTCAAGGCCGCGTACGTCTGTCAATTAAAGAAG
CACAAACTAAAGAAACTGCCGCTGCTGAATAA

Upstream 100 bases:

>100_bases
TTAGTGAAAAGTTGCGTATTTGTCATCATTATCTGCTCTTTATCTATTGATCCGATTTGCAGTATACTTACGCGCGTAAT
TCAAGGTCATTAATTAAGGA

Downstream 100 bases:

>100_bases
TGAGTCTTTGACTTATTATTGGCACACACTTTCTAAGTGATTGCATCAAAAGGAGATCAAATGATCTCCTTTTTTGTTTT
TGGCATTAAGAAAGTCAGTA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MGHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFPLTVNYQEKTYAAGKIPGGFF
KREGRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYID
GEYVLNPSVAQLATSQLNLVVAGTAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQ
DADLVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLGSLEKKVVRGRIIRGEPRIDG
REPDMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKR
REIGHGKLAWRGINAVMPSAAEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITVI
KINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAF
VNILPGKDGLVHISQISDERVANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE

Sequences:

>Translated_703_residues
MGHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFPLTVNYQEKTYAAGKIPGGFF
KREGRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYID
GEYVLNPSVAQLATSQLNLVVAGTAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQ
DADLVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLGSLEKKVVRGRIIRGEPRIDG
REPDMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKR
REIGHGKLAWRGINAVMPSAAEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITVI
KINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAF
VNILPGKDGLVHISQISDERVANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE
>Mature_702_residues
GHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFPLTVNYQEKTYAAGKIPGGFFK
REGRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYIDG
EYVLNPSVAQLATSQLNLVVAGTAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQD
ADLVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLGSLEKKVVRGRIIRGEPRIDGR
EPDMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRR
EIGHGKLAWRGINAVMPSAAEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLS
DILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITVIK
INPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFV
NILPGKDGLVHISQISDERVANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=37.7094972067039, Blast_Score=434, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=73.3719247467438, Blast_Score=1040, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=678, Percent_Identity=32.8908554572271, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI281362905, Length=711, Percent_Identity=36.8495077355837, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=711, Percent_Identity=36.8495077355837, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=711, Percent_Identity=36.8495077355837, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.5296803652968, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 75620; Mature: 75489

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFP
CCCCCHHHHHHHCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEE
LTVNYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVV
EEEECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEE
SVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYIDGEYVLNPSVAQLATSQLNLV
ECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHHHHHCCEEEE
VAGTAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQ
EEECCCEEEEEECHHHHCCHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
DADLVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLG
CHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SLEKKVVRGRIIRGEPRIDGREPDMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGT
HHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCC
ERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSA
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCC
AEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
CCCCEEEHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMD
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHH
QAIGSHRDDISDHAPRITVIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIA
HHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEE
SSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDER
CCCCHHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHH
VANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE
HCCCHHHHHCCHHHEEEEEEECCCCCEEEEEEHHCCCHHCCCC
>Mature Secondary Structure 
GHVNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFP
CCCCHHHHHHHCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEE
LTVNYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVV
EEEECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEE
SVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYIDGEYVLNPSVAQLATSQLNLV
ECCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHHHHHCCEEEE
VAGTAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQ
EEECCCEEEEEECHHHHCCHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
DADLVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLG
CHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHH
SLEKKVVRGRIIRGEPRIDGREPDMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGT
HHHHHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCC
ERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSA
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCC
AEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVL
CCCCEEEHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEE
SDILGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMD
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHH
QAIGSHRDDISDHAPRITVIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIA
HHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEE
SSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDER
CCCCHHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHH
VANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE
HCCCHHHHHCCHHHEEEEEEECCCCCEEEEEEHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA