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The map label for this gene is radA [H]

Identifier: 126175419

GI number: 126175419

Start: 3771708

End: 3773072

Strand: Reverse

Name: radA [H]

Synonym: Sbal_3219

Alternate gene names: 126175419

Gene position: 3773072-3771708 (Counterclockwise)

Preceding gene: 126175421

Following gene: 126175417

Centisome position: 73.59

GC content: 51.5

Gene sequence:

>1365_bases
ATGGCAAAGAATAAAACAGCGTACGTGTGTAATGAGTGTGGACAAGACTTCCCCCGTTGGCAAGGGCAATGCAGTGCTTG
TCAGGAATGGAATACGATTACTGAGGTCAGGCTCGGTGCGGCTTCCCCTGGGCGCAGTACTAAGTTTGTCGGCTATGCCG
GCGCGAGCAGTAATGAAGTGAAGACATTAGATCAAATCGATCTCAATGCTATGCCACGAATATTGAGCCATTTCGGGGAG
TTAGACCGAGTGCTCGGTGGCGGGATAGTGCCAGGTTCAGCCATTTTGATTGGCGGACATCCTGGTGCGGGTAAGAGTAC
GCTTTTATTGCAAACCCTGTGTCATCTGGCCGAGCAAATGCCTGCACTGTATGTCACGGGTGAAGAGTCCTTGCAGCAAG
TGGCCATGCGTGCCCATCGCCTTGGTTTACCAACCAATAAACTGCGGATGTTATCTGAAACCAGCGTTGAACAAATTTGC
GAAGTTGCCCTAAAAGAATTGCCAAAGGTCATAGTGGTCGATTCGATTCAAGTGATGCACATGAGCGATGTGGCATCGAG
CCCCGGTAGTGTGTCGCAGGTCCGTGAATCCGCCTCTTACTTAACGCGTTTTGCGAAGGAGAATGGCATTGCCGTCATCA
TGGTCGGCCATGTGACGAAAGATGGCAGTCTGGCGGGACCTAAGGTATTGGAGCATTGCATCGATTGCTCTGTGATGTTC
GAAGGCGATAGTGATAGTCGTTACCGCACCTTGCGTTCCCACAAAAACCGGTTTGGCGCCATCAATGAGTTAGGTGTATT
TGCCATGACAGAGCGCGGCTTAAAAGAAGTCGCCAATCCTTCGGCAATTTTCCTTTCCCGTGGTGAAGAGGAATCTTCTG
GCTCGCTTGTTATGGTGGTTTGGGAAGGCACGCGGCCGCTACTTGTGGAGCTGCAAGTGCTGGTGGATAACTCGGCCATG
TCTAACCCGCGCCGCGTGGCTGTGGGTATGGATGCCAACCGTTTAGCCATGTTGCTTGCTGTGATGCACCGCCACGGCGG
TTTACAGATGTCAGATCAGGATGTATTTGTGAACGTGGTGGGCGGCGTGAAAGTGACAGAAACCAGTGCCGACTTAACGC
TACTCTTGGCTATGGTATCGAGTTTTCGTGGGGATATTTTGCCCAGCGATTTAGTCGCCTTTGGTGAAGTGGGATTATCT
GGCGAAATTCGGCCTGTGCCCAATGGCCAAGAGCGATTAATCGAAGCTGCTAAGCATGGTTTTAAGCGCGCAATTGTGCC
AAAGGCCAATGTGCCGAAAAAGCCTCCAGCAGGGATGGAAGTCGTGGGTGTGTCTAAGTTATCCGAAGCGCTAGAAGCGA
TTTAG

Upstream 100 bases:

>100_bases
TCTGCATCTTCAGGTTGTTTGGGTATATGTTGTACTGTTAGTCTTGTGTCGGCAGTATTCATCGTTACTTAAATTGATTT
TATACAGATTTTAATGAGTT

Downstream 100 bases:

>100_bases
TCATTAGGCCGAGTAAAATTAGAGGCTAGCTAAATTTAGGCGCTAGCTAACACTTAGATGCTAGCTGGCACTTAGATGCT
AGCTGGCATTAAACGCTCTC

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRSTKFVGYAGASSNEVKTLDQIDLNAMPRILSHFGE
LDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCHLAEQMPALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQIC
EVALKELPKVIVVDSIQVMHMSDVASSPGSVSQVRESASYLTRFAKENGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVVWEGTRPLLVELQVLVDNSAM
SNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVVGGVKVTETSADLTLLLAMVSSFRGDILPSDLVAFGEVGLS
GEIRPVPNGQERLIEAAKHGFKRAIVPKANVPKKPPAGMEVVGVSKLSEALEAI

Sequences:

>Translated_454_residues
MAKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRSTKFVGYAGASSNEVKTLDQIDLNAMPRILSHFGE
LDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCHLAEQMPALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQIC
EVALKELPKVIVVDSIQVMHMSDVASSPGSVSQVRESASYLTRFAKENGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVVWEGTRPLLVELQVLVDNSAM
SNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVVGGVKVTETSADLTLLLAMVSSFRGDILPSDLVAFGEVGLS
GEIRPVPNGQERLIEAAKHGFKRAIVPKANVPKKPPAGMEVVGVSKLSEALEAI
>Mature_453_residues
AKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRSTKFVGYAGASSNEVKTLDQIDLNAMPRILSHFGEL
DRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCHLAEQMPALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQICE
VALKELPKVIVVDSIQVMHMSDVASSPGSVSQVRESASYLTRFAKENGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMFE
GDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVVWEGTRPLLVELQVLVDNSAMS
NPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVVGGVKVTETSADLTLLLAMVSSFRGDILPSDLVAFGEVGLSG
EIRPVPNGQERLIEAAKHGFKRAIVPKANVPKKPPAGMEVVGVSKLSEALEAI

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=455, Percent_Identity=76.2637362637363, Blast_Score=716, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 48727; Mature: 48596

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRSTKFVGYAGASSNEV
CCCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCHHHEEECCCCCCCCCEEEEECCCCCCCC
KTLDQIDLNAMPRILSHFGELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCHLAEQM
HHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
PALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQICEVALKELPKVIVVDSIQVMH
CEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
MSDVASSPGSVSQVRESASYLTRFAKENGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCEEEE
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVV
ECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEE
WEGTRPLLVELQVLVDNSAMSNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVV
ECCCCCEEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
GGVKVTETSADLTLLLAMVSSFRGDILPSDLVAFGEVGLSGEIRPVPNGQERLIEAAKHG
CCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
FKRAIVPKANVPKKPPAGMEVVGVSKLSEALEAI
HHEECCCCCCCCCCCCCCCEEEEHHHHHHHHHCC
>Mature Secondary Structure 
AKNKTAYVCNECGQDFPRWQGQCSACQEWNTITEVRLGAASPGRSTKFVGYAGASSNEV
CCCCCCHHHHHHCCCCCCCCCCCHHHHHCCCHHHEEECCCCCCCCCEEEEECCCCCCCC
KTLDQIDLNAMPRILSHFGELDRVLGGGIVPGSAILIGGHPGAGKSTLLLQTLCHLAEQM
HHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHC
PALYVTGEESLQQVAMRAHRLGLPTNKLRMLSETSVEQICEVALKELPKVIVVDSIQVMH
CEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCEEEEE
MSDVASSPGSVSQVRESASYLTRFAKENGIAVIMVGHVTKDGSLAGPKVLEHCIDCSVMF
HHHHCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCHHHHHHHCCCEEEE
EGDSDSRYRTLRSHKNRFGAINELGVFAMTERGLKEVANPSAIFLSRGEEESSGSLVMVV
ECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEE
WEGTRPLLVELQVLVDNSAMSNPRRVAVGMDANRLAMLLAVMHRHGGLQMSDQDVFVNVV
ECCCCCEEEEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEE
GGVKVTETSADLTLLLAMVSSFRGDILPSDLVAFGEVGLSGEIRPVPNGQERLIEAAKHG
CCEEEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
FKRAIVPKANVPKKPPAGMEVVGVSKLSEALEAI
HHEECCCCCCCCCCCCCCCEEEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1327967; 8759876; 7610040; 9278503 [H]