Click here to switch to the map view.

The map label for this gene is glyA [H]

Identifier: 126175363

GI number: 126175363

Start: 3700783

End: 3702036

Strand: Reverse

Name: glyA [H]

Synonym: Sbal_3162

Alternate gene names: 126175363

Gene position: 3702036-3700783 (Counterclockwise)

Preceding gene: 126175365

Following gene: 126175362

Centisome position: 72.2

GC content: 47.13

Gene sequence:

>1254_bases
ATGCTGAAAAAAGATATGAATATCGCAGATTATGATCCGGAACTGTTTAAGGCAATTCAGAATGAAACTCTGCGTCAAGA
AGAGCATATTGAGCTGATTGCTTCTGAAAACTACACCAGTCCACGCGTGATGGAAGCGCAAGGTTCACAGTTAACCAATA
AGTACGCTGAAGGCTATCCAGGCAAGCGTTACTACGGTGGTTGTGAGTATGTGGACGTAGTTGAAACCTTAGCGATTGAA
CGTGCTAAAGAATTGTTTGGCGCAACTTACGCTAACGTACAACCTCATTCAGGTTCACAGGCAAACAGCGCTGTTTACAT
GGCATTGTTAAAACCTGGCGATACCGTACTGGGCATGAACTTAGCCCACGGTGGTCACTTGACCCACGGTTCACCCGTTA
ACTTCTCTGGCAAACTGTACAATATCATTCCTTACGGTATCGACGAGTCAGGTAAAATTGACTACGACGAAATGGAACGT
CTGGCCGTAGAACATAAGCCTAAAATGATGATCGGCGGTTTCTCTGCCTATTCTGGTATCGTTGACTGGGCAAAAATGCG
TGAAATCGCAGACAAAATAGGTGCTTACCTATTTGTCGATATGGCGCACGTTGCCGGTCTTATCGCTGCGGGTGTTTATC
CAAACCCAGTTCCACACGCGCACGTTGTGACATCAACGACCCATAAAACTTTAGCCGGTCCTCGTGGTGGCGTGATCCTG
TCTGCTGCTGATGATGAAGACTTGTATAAGAAGTTGAATTCAGCGGTATTCCCAGGCGGTCAAGGTGGCCCATTAATGCA
CGTTATCGCTGGTAAAGCGGTTGCGTTCAAAGAAGCACTAGAGCCAGAATTTAAAGTGTACCAACAGCAAGTAGTTAATA
ACGCTAAAGCTATGGTTGAAGTGTTCCTTGAGCGCGGTTACAAAATCGTCTCTGGCGGAACCAGCAACCACTTAATGCTA
GTTGACCTGATTGGTCGTGACTTGACCGGTAAAGAAGCCGATGCCGCTTTAGGTAGCGCTAACATTACCGTGAACAAAAA
CTCAGTGCCAAACGACCCACGTTCACCTTTTGTGACCTCAGGTGTGCGTATCGGTACGCCAGCCATTACGCGCCGCGGCT
TTAAAGAAGTGGAATCGAAAGAGCTGACTGGCTGGATTTGTGACATCCTTGATGATGCAAGCAATCCTGCTGTGATTGAG
CGTGTTAAGGGCCAAGTATTGGCACTGTGTGCACGTTTCCCTGTTTACGGCTAA

Upstream 100 bases:

>100_bases
ATCCACAGGAATTTCATGTTTCAAATACTGGGATTGTGGTCGCGTTCCGAGTAGAATACCGCGCAACCCTACCTATAAGA
ATTGGCAGCTGGAGTAAGCA

Downstream 100 bases:

>100_bases
TTTGCTGACGCATCTAGGTTTAAGTACGTATGTGCTTACGTAGATGATTGGCTGAGAGTGATCGCTTGCTGACTAGGTAA
TTACATGAGGTACTTACATC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLKKDMNIADYDPELFKAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIE
RAKELFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYDEMER
LAVEHKPKMMIGGFSAYSGIVDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVEVFLERGYKIVSGGTSNHLML
VDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEVESKELTGWICDILDDASNPAVIE
RVKGQVLALCARFPVYG

Sequences:

>Translated_417_residues
MLKKDMNIADYDPELFKAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIE
RAKELFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYDEMER
LAVEHKPKMMIGGFSAYSGIVDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVEVFLERGYKIVSGGTSNHLML
VDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEVESKELTGWICDILDDASNPAVIE
RVKGQVLALCARFPVYG
>Mature_417_residues
MLKKDMNIADYDPELFKAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIE
RAKELFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYDEMER
LAVEHKPKMMIGGFSAYSGIVDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVEVFLERGYKIVSGGTSNHLML
VDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEVESKELTGWICDILDDASNPAVIE
RVKGQVLALCARFPVYG

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=411, Percent_Identity=45.4987834549878, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI261862352, Length=411, Percent_Identity=45.4987834549878, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI261862350, Length=411, Percent_Identity=45.4987834549878, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI261862348, Length=411, Percent_Identity=45.4987834549878, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI261862346, Length=410, Percent_Identity=45.1219512195122, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=46.8827930174564, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=44.7300771208226, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=82.9326923076923, Blast_Score=718, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=401, Percent_Identity=45.6359102244389, Blast_Score=341, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI25144729, Length=401, Percent_Identity=45.6359102244389, Blast_Score=340, Evalue=7e-94,
Organism=Saccharomyces cerevisiae, GI6319739, Length=415, Percent_Identity=44.8192771084337, Blast_Score=361, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=45.273631840796, Blast_Score=338, Evalue=8e-94,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=47.25, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=47.25, Blast_Score=363, Evalue=1e-101,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45208; Mature: 45208

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKDMNIADYDPELFKAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCEEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDVVETLAIERAKELFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYDEMERLAVEHKPKMMIGGFSAYSGI
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVE
EECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLERGYKIVSGGTSNHLMLVDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEEC
GVRIGTPAITRRGFKEVESKELTGWICDILDDASNPAVIERVKGQVLALCARFPVYG
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKKDMNIADYDPELFKAIQNETLRQEEHIELIASENYTSPRVMEAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCEEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDVVETLAIERAKELFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAHGGHLTHGSPVNFSGKLYNIIPYGIDESGKIDYDEMERLAVEHKPKMMIGGFSAYSGI
ECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWAKMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKVYQQQVVNNAKAMVE
EECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLERGYKIVSGGTSNHLMLVDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEEC
GVRIGTPAITRRGFKEVESKELTGWICDILDDASNPAVIERVKGQVLALCARFPVYG
CEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA