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The map label for this gene is gatA [H]

Identifier: 126175332

GI number: 126175332

Start: 3667206

End: 3668912

Strand: Direct

Name: gatA [H]

Synonym: Sbal_3131

Alternate gene names: 126175332

Gene position: 3667206-3668912 (Clockwise)

Preceding gene: 126175331

Following gene: 126175339

Centisome position: 71.52

GC content: 54.48

Gene sequence:

>1707_bases
ATGTTTGAAGTCACTGAAGTTTCTATTGCCGAGCTGCGTGCAGCACTCGAATCCGGTCGCACCACAGCAGTTGAGCTGGT
GAAGTCTTATCTCGCACGGGTTGAGGCTTATGACGGCCCAGCTACGCAGACCAAGCTTAATGCGTTAGTGGTCCACAATC
CCGATGCGCTCAAAGAAGCCGAGGCGTCAGATGCGCGCCGCGCCCGTGGTGAAACACTGAGCCCACTTGAGGGTATTACG
TATACCGCCAAGGATAGCTATTTGGTGAAGGGACTGACGGCGGCGTCTGGCAGCCCAGCATTTAAAGATTTAGTGGCGCA
ATACGATGCCTTTGCCGTTGAGCGCCTGCGCGCCGCGGGTGCCATTTGCTTAGGCAAAACCAATATGCCACCCATGGCCA
ATGGCGGTATGCAGCGTGGTCACTATGGCCGCGCTGAAAGCCCATACAATGCTGAGTATCTTACTGCACCATTCGCTTCG
GGTTCGTCTAACGGCGCAGGTACAGGAACCGCCGCCAGCTATTCTGCTTTCGGTCTTGCTGAAGAAACTTGGTCGAGCGG
CCGAGGTCCGGCATCTAATAACGGTTTGTGTGCTTACACTCCGTCACGGGGCGTGATTTCCGTGCGCGGTAACTGGCCGT
TAACGCCGACCATGGATGTCGTCGTGCCTTATACCCGCACTATGGCAGACATGCTCGAAGTGCTGGATGTGATTGTGGCT
GACGATCCTATCACCCGTGGCGACCTCTGGCGTCTACAAACTTGGGTCGAGATCCCTAAAGCATCAAGTGTCCGTCCAGC
ATCTTATCTGGAGCTTGCCGCTCAAGCCGACACGCTTAAAGGTAAGCGTTTTGGTGTGCCGCGCATGTACATCAATAAGG
ATGAATTGGCCGGCACCAGCGAAAATCCGGGCATTGGCGGGCCAACGGGCCAACGTATCCACACTCGCCAATCGGTTATC
GATTTGTGGGAAGCGGCTCGTAAAGCCTTAGAAGCTGCCGGCGCCGAAGTGATTGAAGTCGATTTTCCGCTGGTTTCCAA
TTGTGAAGGCGACCGCCCAGGCGCGCCGACCGTGTTTAACCGTGGCATAGTCACGCCCCAGTTCCTCCATGATGAATTAT
GGGAACTCTCGGGTTGGGCGTTCGATGAATTCCTGCGGGCCAATAATGACCCTAAACTCAACAAACTTGCTGATGTTGTT
GGGCATTTGATTTTCCCCCACGACTTAGGCACTTTGCCAAACCGTGAAGGTGACTTAGCGGCAGGCATGGAAGAGTACGT
CAACATGGCTAAGCGTGGTCTTAAATCCTATGACCAGATCGACAGTATTCCCGATGGTCTGCGTGGCTTAGAGAAGACGC
GTAAGCTAGATCTCGAAGACTGGATGGACGGGCTAAAGCTGGATGCAGTGCTGTTCCCAACCGTGGCAGATGTCGGCCCA
GCCGATGCCCATGTGAATCCGGCTTCTGCCGATATCGCTTGGAGCAACGGAGTTTGGGTCGCTAACGGTAACCTCGCGAT
TCGTCACTTAGGGGTACCGACTGTGACTGTGCCTATGGGCGTGATGGCCGATATTGGTATGCCAGTGGGGTTAACCTTCG
CGGGCCGTGCCTATGACGATAATAACCTGCTTAAATTTGCAGGTGCATTTGAGTCAACAGATGAAAGGCGCATGATCCCG
CCACGCACACCAAAGCTTGGTGAATAA

Upstream 100 bases:

>100_bases
ACAGATCCCCTCTGGAAAGTAAGTCAACCTAAGGAGAACACTATGACGCTTGATGTCACTGTTGTTGCAACACAATTGGC
TTTCTGCGGGAGGGCGGAAG

Downstream 100 bases:

>100_bases
GTGCTAAGACAATGCATAAGCCTATGTAAGCACTAAATGCATGAGCTTAGAAATAAAAACGGGATTGGCATTAAGATGCC
AATCCCGTTTTTTTTAATAC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQTKLNALVVHNPDALKEAEASDARRARGETLSPLEGIT
YTAKDSYLVKGLTAASGSPAFKDLVAQYDAFAVERLRAAGAICLGKTNMPPMANGGMQRGHYGRAESPYNAEYLTAPFAS
GSSNGAGTGTAASYSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYTRTMADMLEVLDVIVA
DDPITRGDLWRLQTWVEIPKASSVRPASYLELAAQADTLKGKRFGVPRMYINKDELAGTSENPGIGGPTGQRIHTRQSVI
DLWEAARKALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVTPQFLHDELWELSGWAFDEFLRANNDPKLNKLADVV
GHLIFPHDLGTLPNREGDLAAGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDGLKLDAVLFPTVADVGP
ADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDDNNLLKFAGAFESTDERRMIP
PRTPKLGE

Sequences:

>Translated_568_residues
MFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQTKLNALVVHNPDALKEAEASDARRARGETLSPLEGIT
YTAKDSYLVKGLTAASGSPAFKDLVAQYDAFAVERLRAAGAICLGKTNMPPMANGGMQRGHYGRAESPYNAEYLTAPFAS
GSSNGAGTGTAASYSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYTRTMADMLEVLDVIVA
DDPITRGDLWRLQTWVEIPKASSVRPASYLELAAQADTLKGKRFGVPRMYINKDELAGTSENPGIGGPTGQRIHTRQSVI
DLWEAARKALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVTPQFLHDELWELSGWAFDEFLRANNDPKLNKLADVV
GHLIFPHDLGTLPNREGDLAAGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDGLKLDAVLFPTVADVGP
ADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDDNNLLKFAGAFESTDERRMIP
PRTPKLGE
>Mature_568_residues
MFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQTKLNALVVHNPDALKEAEASDARRARGETLSPLEGIT
YTAKDSYLVKGLTAASGSPAFKDLVAQYDAFAVERLRAAGAICLGKTNMPPMANGGMQRGHYGRAESPYNAEYLTAPFAS
GSSNGAGTGTAASYSAFGLAEETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYTRTMADMLEVLDVIVA
DDPITRGDLWRLQTWVEIPKASSVRPASYLELAAQADTLKGKRFGVPRMYINKDELAGTSENPGIGGPTGQRIHTRQSVI
DLWEAARKALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFNRGIVTPQFLHDELWELSGWAFDEFLRANNDPKLNKLADVV
GHLIFPHDLGTLPNREGDLAAGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDGLKLDAVLFPTVADVGP
ADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDDNNLLKFAGAFESTDERRMIP
PRTPKLGE

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=360, Percent_Identity=29.4444444444444, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI195972892, Length=241, Percent_Identity=28.2157676348548, Blast_Score=90, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=268, Percent_Identity=25.3731343283582, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17537465, Length=253, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=178, Percent_Identity=31.4606741573034, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648113, Length=304, Percent_Identity=26.6447368421053, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI161078093, Length=254, Percent_Identity=33.4645669291339, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24644968, Length=251, Percent_Identity=30.6772908366534, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI21356731, Length=245, Percent_Identity=27.3469387755102, Blast_Score=72, Evalue=8e-13,
Organism=Drosophila melanogaster, GI24652985, Length=216, Percent_Identity=32.4074074074074, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922090, Length=216, Percent_Identity=32.4074074074074, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652981, Length=216, Percent_Identity=32.4074074074074, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652983, Length=216, Percent_Identity=32.4074074074074, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24648435, Length=252, Percent_Identity=26.5873015873016, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24648437, Length=252, Percent_Identity=26.5873015873016, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45550774, Length=257, Percent_Identity=26.8482490272374, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 61080; Mature: 61080

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQTKLNALVVHNPDALKEA
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCHHHHHH
EASDARRARGETLSPLEGITYTAKDSYLVKGLTAASGSPAFKDLVAQYDAFAVERLRAAG
HHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
AICLGKTNMPPMANGGMQRGHYGRAESPYNAEYLTAPFASGSSNGAGTGTAASYSAFGLA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCC
EETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYTRTMADMLEVLDVIVA
HHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCHHEEECHHHHHHHHHHHHHHHHC
DDPITRGDLWRLQTWVEIPKASSVRPASYLELAAQADTLKGKRFGVPRMYINKDELAGTS
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECHHHHCCCC
ENPGIGGPTGQRIHTRQSVIDLWEAARKALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCCCHHHC
RGIVTPQFLHDELWELSGWAFDEFLRANNDPKLNKLADVVGHLIFPHDLGTLPNREGDLA
CCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHH
AGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDGLKLDAVLFPTVADVGP
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCEECEEECCHHHCCCC
ADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDD
CCCCCCCCCCCEEECCCEEEECCCEEEEEECCCEEEECHHHHHHCCCCCCEEEECCEECC
NNLLKFAGAFESTDERRMIPPRTPKLGE
CCEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFEVTEVSIAELRAALESGRTTAVELVKSYLARVEAYDGPATQTKLNALVVHNPDALKEA
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCHHHHHH
EASDARRARGETLSPLEGITYTAKDSYLVKGLTAASGSPAFKDLVAQYDAFAVERLRAAG
HHHHHHHHCCCCCCCCCCCEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
AICLGKTNMPPMANGGMQRGHYGRAESPYNAEYLTAPFASGSSNGAGTGTAASYSAFGLA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCHHHHCCC
EETWSSGRGPASNNGLCAYTPSRGVISVRGNWPLTPTMDVVVPYTRTMADMLEVLDVIVA
HHHHCCCCCCCCCCCEEEECCCCCEEEECCCCCCCCCHHEEECHHHHHHHHHHHHHHHHC
DDPITRGDLWRLQTWVEIPKASSVRPASYLELAAQADTLKGKRFGVPRMYINKDELAGTS
CCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEECHHHHCCCC
ENPGIGGPTGQRIHTRQSVIDLWEAARKALEAAGAEVIEVDFPLVSNCEGDRPGAPTVFN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCCCHHHC
RGIVTPQFLHDELWELSGWAFDEFLRANNDPKLNKLADVVGHLIFPHDLGTLPNREGDLA
CCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHH
AGMEEYVNMAKRGLKSYDQIDSIPDGLRGLEKTRKLDLEDWMDGLKLDAVLFPTVADVGP
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHCCCEECEEECCHHHCCCC
ADAHVNPASADIAWSNGVWVANGNLAIRHLGVPTVTVPMGVMADIGMPVGLTFAGRAYDD
CCCCCCCCCCCEEECCCEEEECCCEEEEEECCCEEEECHHHHHHCCCCCCEEEECCEECC
NNLLKFAGAFESTDERRMIPPRTPKLGE
CCEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA