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The map label for this gene is yhaR [H]

Identifier: 126175035

GI number: 126175035

Start: 3330975

End: 3331847

Strand: Direct

Name: yhaR [H]

Synonym: Sbal_2830

Alternate gene names: 126175035

Gene position: 3330975-3331847 (Clockwise)

Preceding gene: 126175034

Following gene: 126175036

Centisome position: 64.96

GC content: 49.83

Gene sequence:

>873_bases
ATGACAGACAGATTAAACAGCACTAGCGGCACAAAGGATTTAAGCAACTTGCAGCACGTCACTTATGCGCTCAATAACGG
CGTTGCCGAGCTTATTCTTGACCGAGCAGAAGTACACAACGCCTTCGATGAAGTGATGATTAGCGAGATGATTAGCGTCC
TTAGCTATTTTGCTGAGCACAAGCAATGCCAAGTGCTGGTACTCAAAGCAAACGGCAAAAACTTCAGCGCCGGCGCCGAT
CTCAATTGGATGCGCAAACAAGCCAAAATGGACTTTGAGCAAAATCTAAATGATGCCAAAGCCTTAGCAAAACTGATGCA
GGATTTAGATACCTTTCCTAAGCCCACCATTGCATTAGTGCAAGGCGCAGCCTTTGGCGGCGCACTGGGGCTGATTTGCG
CCAGCGATATCGCTATCGCCACAACGCGCGCCAGTTTTTGCTTAAGCGAAGTGAAACTCGGATTAATTCCTGCGGTGATA
AGCCCATACGTTGCCCGCGCTATGGGTAACCGCGCATCGCGCCGCTACATGTTAACGGCTGAGCGTTTTAGTGCAGAAAA
CGCATTAAAGCTTAATGTCATTCATGAGATCAGCGATGACTTGGACGCCGCCGCAAAGCCTATGATTGACGCGCTACTTG
CCAATAGTCCGCAAGGCATGGCGTGGGTAAAAACACTGCTGTCGCATCTTGAAGATGGCGTGATAGACCAAAACACCATT
GATTACACTAGCGAGCGTATCGCCCGCATTCGGGTATCAACCGAGGGCCAAGAAGGCTTAAACGCCTTTTTTGAGAAACG
TCTGCCTAACTGGCAACAAGCGCTCTCAACTCCCCCCACGCAAAAGCCAAGTGATACCCAAGGAGCCCTTTGA

Upstream 100 bases:

>100_bases
GCGTTAAACGCCCCTATCGAAGACACCCGCTTTGGTGTGTTCCGTATGTAAAGGCACATGTGAAAAACATATGCCACACA
ATAAGATAGGGGAAAGAATA

Downstream 100 bases:

>100_bases
TGTTAACCAAGCAAATGTTTACCAAGCTATTGATTGCTAACCGCGGTGAAATCGCCTGTCGCATCATCAAAACCGCCCAT
GTCATGGGCGTTCGCACCGT

Product: enoyl-CoA hydratase/isomerase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTDRLNSTSGTKDLSNLQHVTYALNNGVAELILDRAEVHNAFDEVMISEMISVLSYFAEHKQCQVLVLKANGKNFSAGAD
LNWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKPTIALVQGAAFGGALGLICASDIAIATTRASFCLSEVKLGLIPAVI
SPYVARAMGNRASRRYMLTAERFSAENALKLNVIHEISDDLDAAAKPMIDALLANSPQGMAWVKTLLSHLEDGVIDQNTI
DYTSERIARIRVSTEGQEGLNAFFEKRLPNWQQALSTPPTQKPSDTQGAL

Sequences:

>Translated_290_residues
MTDRLNSTSGTKDLSNLQHVTYALNNGVAELILDRAEVHNAFDEVMISEMISVLSYFAEHKQCQVLVLKANGKNFSAGAD
LNWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKPTIALVQGAAFGGALGLICASDIAIATTRASFCLSEVKLGLIPAVI
SPYVARAMGNRASRRYMLTAERFSAENALKLNVIHEISDDLDAAAKPMIDALLANSPQGMAWVKTLLSHLEDGVIDQNTI
DYTSERIARIRVSTEGQEGLNAFFEKRLPNWQQALSTPPTQKPSDTQGAL
>Mature_289_residues
TDRLNSTSGTKDLSNLQHVTYALNNGVAELILDRAEVHNAFDEVMISEMISVLSYFAEHKQCQVLVLKANGKNFSAGADL
NWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKPTIALVQGAAFGGALGLICASDIAIATTRASFCLSEVKLGLIPAVIS
PYVARAMGNRASRRYMLTAERFSAENALKLNVIHEISDDLDAAAKPMIDALLANSPQGMAWVKTLLSHLEDGVIDQNTID
YTSERIARIRVSTEGQEGLNAFFEKRLPNWQQALSTPPTQKPSDTQGAL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=25.3968253968254, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI194097323, Length=256, Percent_Identity=29.296875, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87082183, Length=262, Percent_Identity=27.0992366412214, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=27.3469387755102, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788597, Length=261, Percent_Identity=25.6704980842912, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI221142681, Length=275, Percent_Identity=27.6363636363636, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787660, Length=272, Percent_Identity=27.9411764705882, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790281, Length=175, Percent_Identity=26.8571428571429, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17540714, Length=180, Percent_Identity=29.4444444444444, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=26.0536398467433, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=233, Percent_Identity=25.3218884120172, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=250, Percent_Identity=26, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17549921, Length=221, Percent_Identity=27.1493212669683, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536985, Length=222, Percent_Identity=25.2252252252252, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=27.906976744186, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19920382, Length=182, Percent_Identity=29.1208791208791, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20129971, Length=269, Percent_Identity=25.2788104089219, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653477, Length=269, Percent_Identity=25.2788104089219, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922422, Length=253, Percent_Identity=22.5296442687747, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 31715; Mature: 31583

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDRLNSTSGTKDLSNLQHVTYALNNGVAELILDRAEVHNAFDEVMISEMISVLSYFAEH
CCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KQCQVLVLKANGKNFSAGADLNWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKPTIALV
CCEEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
QGAAFGGALGLICASDIAIATTRASFCLSEVKLGLIPAVISPYVARAMGNRASRRYMLTA
HCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEH
ERFSAENALKLNVIHEISDDLDAAAKPMIDALLANSPQGMAWVKTLLSHLEDGVIDQNTI
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCH
DYTSERIARIRVSTEGQEGLNAFFEKRLPNWQQALSTPPTQKPSDTQGAL
HHHHHHHHHEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TDRLNSTSGTKDLSNLQHVTYALNNGVAELILDRAEVHNAFDEVMISEMISVLSYFAEH
CCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KQCQVLVLKANGKNFSAGADLNWMRKQAKMDFEQNLNDAKALAKLMQDLDTFPKPTIALV
CCEEEEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHCCCCCHHHHH
QGAAFGGALGLICASDIAIATTRASFCLSEVKLGLIPAVISPYVARAMGNRASRRYMLTA
HCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCEEEEEH
ERFSAENALKLNVIHEISDDLDAAAKPMIDALLANSPQGMAWVKTLLSHLEDGVIDQNTI
HHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCH
DYTSERIARIRVSTEGQEGLNAFFEKRLPNWQQALSTPPTQKPSDTQGAL
HHHHHHHHHEEECCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 9579061 [H]