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The map label for this gene is dctD [H]

Identifier: 126175016

GI number: 126175016

Start: 3305719

End: 3307098

Strand: Direct

Name: dctD [H]

Synonym: Sbal_2810

Alternate gene names: 126175016

Gene position: 3305719-3307098 (Clockwise)

Preceding gene: 126175015

Following gene: 126175018

Centisome position: 64.47

GC content: 49.35

Gene sequence:

>1380_bases
ATGAGTGTGCCCAATTCCATCCAAGATTATTCTGTTCTCATCATCGACGATGAGCCACACATAGGCACAGTTCTCGTTCA
GCTATTTGAACTTGAGGGCATTAAGGCGCTCGCCACCACAGAGCCTAAAGGGATCGCTAAGCACCTGCATAAAGATTGGG
CGGGAATGGTGATATCAGATGTCAACATGCCGCAATTAGATGGCATCAGCTTAATGCAACAAATCCGCCTGCAGGACCCC
GACCTCCCCGTTGTGTTATTGACAGGGTTTGGGGATATCGCCATGGCAGTCAATGCCTTAAAGCAAGGTGCCTACGACTT
TCTCGAAAAGCCCTTTAATAACGAGCATATACTCGATGTGGTCAAGCGCGCCTTAGATAAACGCAGCCTGACCCTAGAGA
ACCGTAAGCTTAAAAAGGATCTCGAAAGTCAGAGCGTACCAGGCCCTCGCATTTTGGGTAACAGTGTCGGCATCAACCGC
ATGCGCCACATCATAGATCAAGTGATAGACACCCCAGCTGATGTGTTAATCGAAGGCGAAACAGGCACAGGTAAAGAGCT
CGTCGCCCGCTATTTACACGATCACAGCAGCCGCAGCCAAGCCAACTTTGTCGCCATCAACTGTGGTGCGATCCCTGAAA
ACTTAATTGAAAGTGAGCTCTTTGGCGCCGAAGCGGGTGCCTTTACCGGCGTCGATAAGATGCGCATTGGTAAGTTTGAA
TACGCCAACGGCGGTACATTATTTTTAGATGAAATTGAAAGTACACCACTGTCGCTGCAAGTAAAACTGCTGCGGGTACT
TGAAGATCGTAAAGTTGAACGCTTAGGGTCAAACAAAAGCATCAGCCTTGATATACGGGTAATTGCCGCCACTAAGGTGG
ATTTAAAAAGCTTGTGTGACACGGGGGAATTTCGCCAAGACTTGTTGTATCGCTTAAATCTGGTGACTGTACCTATCCCT
CCCCTGCGGGATCGCCGTGAGGACATTCCTCTGCTGTTCCTGCACTTTGCACGGGTTGCATCGGCACGCTATCACAAAGC
GCTTATCGCCTTAAATGCCGAGCAAAACGCCATACTCTCCTCCCACGACTGGCCGGGCAATGTGCGTGAGTTACGTAATC
TCGCCGAACGCTTTGTGCTACTAGGTGGAGAAGCTGCCTTTGCTGGCACTTTGGGCAACACCCCAAGCCTGCACTCGAGT
ATGTCCTTACTGCAACGAGTCGAGTTTTTTGAAAGGGTATTGATTGAAGAAGCGCTTAGCCACAACAAGGGCAGCATTAA
GCAAACAATGGAGCAGCTGGAACTGCCTCGCAAAACCCTCTACGACAAAATGCGAAAATACGATTTAGACCGCAAGCAGT
ACATTAACTCCGATGACTAG

Upstream 100 bases:

>100_bases
GAATCCATGCAAGGCCAAATTAACGTTGCCAATGCCGAAGAGGGTGGCGCCATTTTCCAGATTATTTTACCTATTTATTT
GCTTGAGGAACGTTAATCTT

Downstream 100 bases:

>100_bases
CTCATTTCGATGACAAGAGAGTGCGAACCCAGCTTGAGCTGGGTTCGCTATCACAGCATAAAAATGAGTCTGAATCACGT
ATTACGTCGACGTTTTATTA

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSVPNSIQDYSVLIIDDEPHIGTVLVQLFELEGIKALATTEPKGIAKHLHKDWAGMVISDVNMPQLDGISLMQQIRLQDP
DLPVVLLTGFGDIAMAVNALKQGAYDFLEKPFNNEHILDVVKRALDKRSLTLENRKLKKDLESQSVPGPRILGNSVGINR
MRHIIDQVIDTPADVLIEGETGTGKELVARYLHDHSSRSQANFVAINCGAIPENLIESELFGAEAGAFTGVDKMRIGKFE
YANGGTLFLDEIESTPLSLQVKLLRVLEDRKVERLGSNKSISLDIRVIAATKVDLKSLCDTGEFRQDLLYRLNLVTVPIP
PLRDRREDIPLLFLHFARVASARYHKALIALNAEQNAILSSHDWPGNVRELRNLAERFVLLGGEAAFAGTLGNTPSLHSS
MSLLQRVEFFERVLIEEALSHNKGSIKQTMEQLELPRKTLYDKMRKYDLDRKQYINSDD

Sequences:

>Translated_459_residues
MSVPNSIQDYSVLIIDDEPHIGTVLVQLFELEGIKALATTEPKGIAKHLHKDWAGMVISDVNMPQLDGISLMQQIRLQDP
DLPVVLLTGFGDIAMAVNALKQGAYDFLEKPFNNEHILDVVKRALDKRSLTLENRKLKKDLESQSVPGPRILGNSVGINR
MRHIIDQVIDTPADVLIEGETGTGKELVARYLHDHSSRSQANFVAINCGAIPENLIESELFGAEAGAFTGVDKMRIGKFE
YANGGTLFLDEIESTPLSLQVKLLRVLEDRKVERLGSNKSISLDIRVIAATKVDLKSLCDTGEFRQDLLYRLNLVTVPIP
PLRDRREDIPLLFLHFARVASARYHKALIALNAEQNAILSSHDWPGNVRELRNLAERFVLLGGEAAFAGTLGNTPSLHSS
MSLLQRVEFFERVLIEEALSHNKGSIKQTMEQLELPRKTLYDKMRKYDLDRKQYINSDD
>Mature_458_residues
SVPNSIQDYSVLIIDDEPHIGTVLVQLFELEGIKALATTEPKGIAKHLHKDWAGMVISDVNMPQLDGISLMQQIRLQDPD
LPVVLLTGFGDIAMAVNALKQGAYDFLEKPFNNEHILDVVKRALDKRSLTLENRKLKKDLESQSVPGPRILGNSVGINRM
RHIIDQVIDTPADVLIEGETGTGKELVARYLHDHSSRSQANFVAINCGAIPENLIESELFGAEAGAFTGVDKMRIGKFEY
ANGGTLFLDEIESTPLSLQVKLLRVLEDRKVERLGSNKSISLDIRVIAATKVDLKSLCDTGEFRQDLLYRLNLVTVPIPP
LRDRREDIPLLFLHFARVASARYHKALIALNAEQNAILSSHDWPGNVRELRNLAERFVLLGGEAAFAGTLGNTPSLHSSM
SLLQRVEFFERVLIEEALSHNKGSIKQTMEQLELPRKTLYDKMRKYDLDRKQYINSDD

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=38.9010989010989, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1790437, Length=442, Percent_Identity=38.4615384615385, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=36.4605543710021, Blast_Score=287, Evalue=8e-79,
Organism=Escherichia coli, GI1788905, Length=446, Percent_Identity=36.322869955157, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI87082117, Length=272, Percent_Identity=48.1617647058824, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789087, Length=339, Percent_Identity=41.2979351032448, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1789233, Length=321, Percent_Identity=39.8753894080997, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786524, Length=244, Percent_Identity=46.7213114754098, Blast_Score=204, Evalue=8e-54,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=37.4613003095975, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI87081872, Length=235, Percent_Identity=44.2553191489362, Blast_Score=194, Evalue=1e-50,
Organism=Escherichia coli, GI1787583, Length=312, Percent_Identity=36.2179487179487, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI87081858, Length=308, Percent_Identity=30.8441558441558, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789828, Length=228, Percent_Identity=38.1578947368421, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51360; Mature: 51229

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPNSIQDYSVLIIDDEPHIGTVLVQLFELEGIKALATTEPKGIAKHLHKDWAGMVISD
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCEEEC
VNMPQLDGISLMQQIRLQDPDLPVVLLTGFGDIAMAVNALKQGAYDFLEKPFNNEHILDV
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VKRALDKRSLTLENRKLKKDLESQSVPGPRILGNSVGINRMRHIIDQVIDTPADVLIEGE
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
TGTGKELVARYLHDHSSRSQANFVAINCGAIPENLIESELFGAEAGAFTGVDKMRIGKFE
CCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHEECCEE
YANGGTLFLDEIESTPLSLQVKLLRVLEDRKVERLGSNKSISLDIRVIAATKVDLKSLCD
ECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECHHHHHHHHC
TGEFRQDLLYRLNLVTVPIPPLRDRREDIPLLFLHFARVASARYHKALIALNAEQNAILS
CHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEC
SHDWPGNVRELRNLAERFVLLGGEAAFAGTLGNTPSLHSSMSLLQRVEFFERVLIEEALS
CCCCCCCHHHHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
HNKGSIKQTMEQLELPRKTLYDKMRKYDLDRKQYINSDD
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCCCCC
>Mature Secondary Structure 
SVPNSIQDYSVLIIDDEPHIGTVLVQLFELEGIKALATTEPKGIAKHLHKDWAGMVISD
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHCCEEEC
VNMPQLDGISLMQQIRLQDPDLPVVLLTGFGDIAMAVNALKQGAYDFLEKPFNNEHILDV
CCCCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VKRALDKRSLTLENRKLKKDLESQSVPGPRILGNSVGINRMRHIIDQVIDTPADVLIEGE
HHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
TGTGKELVARYLHDHSSRSQANFVAINCGAIPENLIESELFGAEAGAFTGVDKMRIGKFE
CCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHEECCEE
YANGGTLFLDEIESTPLSLQVKLLRVLEDRKVERLGSNKSISLDIRVIAATKVDLKSLCD
ECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECHHHHHHHHC
TGEFRQDLLYRLNLVTVPIPPLRDRREDIPLLFLHFARVASARYHKALIALNAEQNAILS
CHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEECCCCCCEEC
SHDWPGNVRELRNLAERFVLLGGEAAFAGTLGNTPSLHSSMSLLQRVEFFERVLIEEALS
CCCCCCCHHHHHHHHHHHEEECCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
HNKGSIKQTMEQLELPRKTLYDKMRKYDLDRKQYINSDD
CCCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]