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The map label for this gene is gltX

Identifier: 126175009

GI number: 126175009

Start: 3294584

End: 3295993

Strand: Reverse

Name: gltX

Synonym: Sbal_2802

Alternate gene names: 126175009

Gene position: 3295993-3294584 (Counterclockwise)

Preceding gene: 126175017

Following gene: 126175007

Centisome position: 64.28

GC content: 44.33

Gene sequence:

>1410_bases
ATGACAACTAAGACGCGTTTTGCCCCTAGTCCTACTGGTTTCTTACATGTTGGTGGTGCCCGTACTGCACTTTATTCTTG
GTTACAAGCTCGTGCCAATAACGGTGAGTTTGTATTACGCATTGAAGACACTGATATTGAGCGTTCGACTCAAGCCGCCT
GCGATGCGATTTTAGAAGGTATGAACTGGTTAGGTTTGACATGGGACGAGGGCCCGTATTACCAAACTAAGCGTTTTGAT
CGTTATAACGAAATCATTGCCCAAATGTTAGCAAAGGGCACTGCATACAAATGCTATTGCTCACGTGAGCGTATTGATGC
GTTAAGAGAAGCACAAGCCGCCAACGGTGAAGCACAAAAGTACGATGGTTGCTGCCGTGACTTGCCTGCTCGTGATACTG
ATGAGCCATTTGTCGTGCGCTTCAAAAATCCTATCGGCGGTTCAGTGGTATTTGATGACCATGTTCGTGGCCGTATTGAA
TTTTCTAACGATGCATTAGATGACTTGATCATCGCCCGTACCGATGGTGTGCCAACCTATAACTTCTGTGTAGTCGTTGA
CGATTGGGATATGGGAATTACTTGTGTGGTTCGTGGTGAAGATCATATCAACAATACGCCGCGTCAAATTAATATCCTTA
AAGCATTAGGGGCGCCAATCCCAGAATATGCTCACGTCTCTATGATTCTCGGTGATGATGGCGCGAAATTATCTAAGCGT
CATGGCGCAGTGAGCGTAATGCAGTACCGTGACGATGGTTATTTGCCAGAAGCACTGTTGAACTACCTAGTACGTTTAGG
TTGGTCACATGGTGATCAAGAGATCTTCTCTTTAGAAGAAATGAAGCAATACTTTAAGCTCGGTGATATCAATAAAGCCG
CTTCTGCCTTTAATACCGATAAACTGGTGTGGTTGAACCAACACTATATAAAGAGTCTTGCCCCAGAATATGTGGCAACA
CACTTACAATGGCACATGGACGATCAAGAAATTGATCTGTCAAACGGCCCAGCATTAGCTGAAGTGGTTACTGCATTAGC
TGAACGCGCTAAGACACTAAAAGAGTTAGCCGCTTCTAGCCGCTATTTCTATGAAGACTTTGCAGAGTTTGATGAAGCCC
AAGCGAAAAAGCATTTACGTGGTGTTGCGTTAGAGCCATTGCAACTGGTTCAACAAAAATTAGCCGCATTAACTGATTGG
ACTGTAGAAGCTATCCATCAGGCGATTGAAGACACTGCAACAGAATTAGATGTGGGTATGGGCAAGGTTGGTATGCCTTT
ACGTGTCGCTGTAACAGGGGCAGGGCAGTCACCAGGCCTTGATATCACATTATTTTTAATCGGAAGAAGCCGTTCTGAGC
AAAGAATATCCAAAGCGATTGAATTTGTAGCAGATAGAATAAATTCCTAA

Upstream 100 bases:

>100_bases
TTGTCCCATTTGCAGCGCCTGCTTTGACTGTTAGAATAAGCCCACTTTTTTATCGTGATTGTCTTTATCTAGACGACTTT
ATATAGGTTAGTGTCCCATT

Downstream 100 bases:

>100_bases
AAAACGAGTTGACACTTTTAGGTGCGCTGCATAGAATGCGCCACGCAGTTGAGACACAGACGAGCAAATGCTTGTAGTGC
ACTTAAATGTAGTTTAATAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEGMNWLGLTWDEGPYYQTKRFD
RYNEIIAQMLAKGTAYKCYCSRERIDALREAQAANGEAQKYDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIE
FSNDALDDLIIARTDGVPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSLEEMKQYFKLGDINKAASAFNTDKLVWLNQHYIKSLAPEYVAT
HLQWHMDDQEIDLSNGPALAEVVTALAERAKTLKELAASSRYFYEDFAEFDEAQAKKHLRGVALEPLQLVQQKLAALTDW
TVEAIHQAIEDTATELDVGMGKVGMPLRVAVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS

Sequences:

>Translated_469_residues
MTTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEGMNWLGLTWDEGPYYQTKRFD
RYNEIIAQMLAKGTAYKCYCSRERIDALREAQAANGEAQKYDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIE
FSNDALDDLIIARTDGVPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSLEEMKQYFKLGDINKAASAFNTDKLVWLNQHYIKSLAPEYVAT
HLQWHMDDQEIDLSNGPALAEVVTALAERAKTLKELAASSRYFYEDFAEFDEAQAKKHLRGVALEPLQLVQQKLAALTDW
TVEAIHQAIEDTATELDVGMGKVGMPLRVAVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS
>Mature_468_residues
TTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEGMNWLGLTWDEGPYYQTKRFDR
YNEIIAQMLAKGTAYKCYCSRERIDALREAQAANGEAQKYDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIEF
SNDALDDLIIARTDGVPTYNFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKRH
GAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSLEEMKQYFKLGDINKAASAFNTDKLVWLNQHYIKSLAPEYVATH
LQWHMDDQEIDLSNGPALAEVVTALAERAKTLKELAASSRYFYEDFAEFDEAQAKKHLRGVALEPLQLVQQKLAALTDWT
VEAIHQAIEDTATELDVGMGKVGMPLRVAVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=332, Percent_Identity=33.7349397590361, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1788743, Length=469, Percent_Identity=73.5607675906183, Blast_Score=727, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=242, Percent_Identity=39.2561983471074, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1786895, Length=218, Percent_Identity=24.7706422018349, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=480, Percent_Identity=29.7916666666667, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=500, Percent_Identity=31.6, Blast_Score=246, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6321192, Length=229, Percent_Identity=27.9475982532751, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324742, Length=120, Percent_Identity=31.6666666666667, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=30.0813008130081, Blast_Score=240, Evalue=1e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_SHEB5 (A3D6C6)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001051158.1
- ProteinModelPortal:   A3D6C6
- SMR:   A3D6C6
- STRING:   A3D6C6
- GeneID:   4843717
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_2802
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   A3D6C6
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52454; Mature: 52323

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
MNWLGLTWDEGPYYQTKRFDRYNEIIAQMLAKGTAYKCYCSRERIDALREAQAANGEAQK
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCHH
YDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIEFSNDALDDLIIARTDGVPTY
HCCHHHCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCHHHEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSLEEMKQYFKLGDINKAASAFNTD
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCC
KLVWLNQHYIKSLAPEYVATHLQWHMDDQEIDLSNGPALAEVVTALAERAKTLKELAASS
EEEEECHHHHHHHCHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
RYFYEDFAEFDEAQAKKHLRGVALEPLQLVQQKLAALTDWTVEAIHQAIEDTATELDVGM
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKVGMPLRVAVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS
CCCCCCEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTKTRFAPSPTGFLHVGGARTALYSWLQARANNGEFVLRIEDTDIERSTQAACDAILEG
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHC
MNWLGLTWDEGPYYQTKRFDRYNEIIAQMLAKGTAYKCYCSRERIDALREAQAANGEAQK
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCHH
YDGCCRDLPARDTDEPFVVRFKNPIGGSVVFDDHVRGRIEFSNDALDDLIIARTDGVPTY
HCCHHHCCCCCCCCCCEEEEECCCCCCCEEEECCCCCEEEECCCCHHHEEEEECCCCCCE
NFCVVVDDWDMGITCVVRGEDHINNTPRQINILKALGAPIPEYAHVSMILGDDGAKLSKR
EEEEEEECCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHH
HGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEIFSLEEMKQYFKLGDINKAASAFNTD
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCC
KLVWLNQHYIKSLAPEYVATHLQWHMDDQEIDLSNGPALAEVVTALAERAKTLKELAASS
EEEEECHHHHHHHCHHHHHHHHHEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCC
RYFYEDFAEFDEAQAKKHLRGVALEPLQLVQQKLAALTDWTVEAIHQAIEDTATELDVGM
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GKVGMPLRVAVTGAGQSPGLDITLFLIGRSRSEQRISKAIEFVADRINS
CCCCCCEEEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA