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The map label for this gene is hcaT [H]

Identifier: 126174958

GI number: 126174958

Start: 3229775

End: 3230950

Strand: Reverse

Name: hcaT [H]

Synonym: Sbal_2751

Alternate gene names: 126174958

Gene position: 3230950-3229775 (Counterclockwise)

Preceding gene: 126174959

Following gene: 126174957

Centisome position: 63.01

GC content: 50.26

Gene sequence:

>1176_bases
ATGTCAACTTCTTCTAGCACAAGCCGGCAATTACGCTGGCTTTGTGCCTGCTATTTTTTCTTCTTTTCTATTCTCGGTAC
TATGGTCCCTTATCTTGGGGTCTTCTTCGAAAATCGCGGCTTTAATCCTCAAGAAATTGGCTTTTTACTCGCCATCCTGA
TGGCGACGCGTATCGTTGCGCCGAATGTATGGGCCAAAGTGGCTGACCGAACCGGGATGCGTGCTGAGCTGATCAAAATG
GGCGCGGGAGCCGCCGCGCTGGCGTATTTGAGTTTTTTCTACCATGGCGGTTTTGTGTATATGGCGCTAAGTCTGGCCCT
CTATACTTTCTTTTGGAATGCGATCCTTGCCCAGCTTGAAGTGATCACCTTAGAAACCTTAGGCGAGAATGCCAGCCGCT
ACGGTCAAATTCGCAGTTGGGGCAGTGTCGGTTATATCTGCCTGGTTGTCGGTGCGGGCTTTGCGATTGGCCAGTGGGGC
ACTGAAGTGCTGCCTTATATCGGTTTTTCATTATTTATCGGCATGTTAGCCAGTGCGTTGCCATTACCAGCAAATCGTTT
AGTGCGTGACACCAGTCAAGTGCGGCCGCCATTACAATGGAATCGGGCGATTATTTGGTTCTTACTCTCGGCTATGTTGC
TGCAAATGAGCGCAGGGCCTTTCTACGGCTTCTTCGTCTTATATTTAAAACAAGCGGGTTACTCAGAATCATCAGCGGGG
ATATTTGTCGCCCTTGGCGCCATGGCCGAAATCGTAATGTTTATGTTCGCGCCGCGTTTACTGGGTCGCTATGGCGTTAA
CACCCTATTGATAGTCAGTATCGGTATGACTTGCTTGCGTTGGTTGTTGGTTGCATTCGGAGTGGACAGCGTATTGTGGC
TTGGTTTTAGTCAATTATTGCATGCCTTTACCTTTGGTTTAACTCACGCGGCATCGATTCAATTTGTGCACAGGCATTTC
GATGCGAGTCACAGAAGCCAAGGTCAAGCCCTATACGCCAGCTTAAGTTTTGGAGTGGGCGGCGCGCTCGGTACTTGGAT
TTGCGGCTATATTTGGGGCGATGGTAGCGGGGCGGTTTGGTCTTGGGTATTTGCAGCCGGATGCGCCTTTTCCGCTATGC
TGGCGGTATTCTTTATTCCTAGGGATAAACCTGGCGTTGCCGCCCATGCGGCCTAA

Upstream 100 bases:

>100_bases
ATGCGATAGGCGCATGAAATAGGGGTATGAGATAGGCGCATGTGATAGATGGTATGCGATAACGATACAACAAGTGGCAT
GAGATAAAGGGAATGCGTTA

Downstream 100 bases:

>100_bases
CAATGTCAATCTAGCAATATCAATCACGATTAAAAGCATCTATTAAGATACTAAAATATAAAGATAAAAATTCCAAGGTT
GCGTTAAGTGAGGTAAAAAG

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSTSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVAPNVWAKVADRTGMRAELIKM
GAGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLEVITLETLGENASRYGQIRSWGSVGYICLVVGAGFAIGQWG
TEVLPYIGFSLFIGMLASALPLPANRLVRDTSQVRPPLQWNRAIIWFLLSAMLLQMSAGPFYGFFVLYLKQAGYSESSAG
IFVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDSVLWLGFSQLLHAFTFGLTHAASIQFVHRHF
DASHRSQGQALYASLSFGVGGALGTWICGYIWGDGSGAVWSWVFAAGCAFSAMLAVFFIPRDKPGVAAHAA

Sequences:

>Translated_391_residues
MSTSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVAPNVWAKVADRTGMRAELIKM
GAGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLEVITLETLGENASRYGQIRSWGSVGYICLVVGAGFAIGQWG
TEVLPYIGFSLFIGMLASALPLPANRLVRDTSQVRPPLQWNRAIIWFLLSAMLLQMSAGPFYGFFVLYLKQAGYSESSAG
IFVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDSVLWLGFSQLLHAFTFGLTHAASIQFVHRHF
DASHRSQGQALYASLSFGVGGALGTWICGYIWGDGSGAVWSWVFAAGCAFSAMLAVFFIPRDKPGVAAHAA
>Mature_390_residues
STSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVAPNVWAKVADRTGMRAELIKMG
AGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLEVITLETLGENASRYGQIRSWGSVGYICLVVGAGFAIGQWGT
EVLPYIGFSLFIGMLASALPLPANRLVRDTSQVRPPLQWNRAIIWFLLSAMLLQMSAGPFYGFFVLYLKQAGYSESSAGI
FVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDSVLWLGFSQLLHAFTFGLTHAASIQFVHRHFD
ASHRSQGQALYASLSFGVGGALGTWICGYIWGDGSGAVWSWVFAAGCAFSAMLAVFFIPRDKPGVAAHAA

Specific function: Probable permease involved in the uptake of 3- phenylpropionic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacY/rafB permease family. HcaT subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788886, Length=394, Percent_Identity=24.6192893401015, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022814
- InterPro:   IPR016196
- InterPro:   IPR005276 [H]

Pfam domain/function: PF01306 LacY_symp [H]

EC number: NA

Molecular weight: Translated: 42776; Mature: 42644

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PNVWAKVADRTGMRAELIKMGAGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLE
CHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VITLETLGENASRYGQIRSWGSVGYICLVVGAGFAIGQWGTEVLPYIGFSLFIGMLASAL
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PLPANRLVRDTSQVRPPLQWNRAIIWFLLSAMLLQMSAGPFYGFFVLYLKQAGYSESSAG
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
IFVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDSVLWLGFSQLL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFTFGLTHAASIQFVHRHFDASHRSQGQALYASLSFGVGGALGTWICGYIWGDGSGAVW
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCCHHHHHHHHHHEEECCCCHHH
SWVFAAGCAFSAMLAVFFIPRDKPGVAAHAA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
STSSSTSRQLRWLCACYFFFFSILGTMVPYLGVFFENRGFNPQEIGFLLAILMATRIVA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PNVWAKVADRTGMRAELIKMGAGAAALAYLSFFYHGGFVYMALSLALYTFFWNAILAQLE
CHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VITLETLGENASRYGQIRSWGSVGYICLVVGAGFAIGQWGTEVLPYIGFSLFIGMLASAL
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
PLPANRLVRDTSQVRPPLQWNRAIIWFLLSAMLLQMSAGPFYGFFVLYLKQAGYSESSAG
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCC
IFVALGAMAEIVMFMFAPRLLGRYGVNTLLIVSIGMTCLRWLLVAFGVDSVLWLGFSQLL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFTFGLTHAASIQFVHRHFDASHRSQGQALYASLSFGVGGALGTWICGYIWGDGSGAVW
HHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEEECCCCCCHHHHHHHHHHEEECCCCHHH
SWVFAAGCAFSAMLAVFFIPRDKPGVAAHAA
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9603882; 9205837; 9278503 [H]