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The map label for this gene is folC [H]

Identifier: 126174948

GI number: 126174948

Start: 3215730

End: 3217001

Strand: Reverse

Name: folC [H]

Synonym: Sbal_2741

Alternate gene names: 126174948

Gene position: 3217001-3215730 (Counterclockwise)

Preceding gene: 126174949

Following gene: 126174947

Centisome position: 62.74

GC content: 49.92

Gene sequence:

>1272_bases
ATGCTAACAGCGCCAGCGTCAAATGCAGCATTGGAACAGTGGTTGGATTATTTGTTATCCATACATCCAACCGAAATCGA
TATGGGATTAACCCGTGTTTCTAAGGTCGCGAAAACCCTTGGCCTATTAGATTTAGGCCAAAGCAAGATAGTCACAGTGG
CAGGCACAAATGGTAAAGGCACGACTTGCGCTATGCTGGAAAATATTCTGCGCTTATCGGGTTTGACTGTGGGCGTATAC
AGCTCGCCGCACATGCTGAAATACAACGAGCGAGTGCGTATTAATGGCAGCGACGCCAGTGATAGCGCCTTTGTCGCCGC
CTTTGAACAAATTGAAGCGGCGCGCGCCGAGATTTCCCTGACCTTTTTTGAATACGCTACGCTTGCGGGCCTGATACTGT
TTAAAGCCGCTAAGCTTGATGTAGTCATCTTAGAAGTCGGTTTAGGTGGACGGTTAGATGCCACCAATATTATCGATGCC
GATATCAGCGTGGTGACGTCGGTCGATTTAGATCATGAAAGTTATCTGGGGAATACCCGTGAACTCGTCGGCCGTGAAAA
GGCGGGTATTTTCCGTCACGGTAAAGCTGCGATTGTCGGCGAGCCCAACTTGCCGCATACCGTCAATGATGTTGCCAATG
ACGTTGGTGCCATCTTGTATCGCGTCGGCAACGAATTCAGTTATCAAGTCCATGGCAATCTGTGGGACTTTCAAGGCCAA
GTGCTCAAACTACACGATTTAGCGTTACCGACCTTGCCGCTACCTAATGCGGCCACGGTATTGGCCGTAATTGAACAGGG
TTGGCCGGATATCGCGCCCGCTATTATTGCCGAGGGTATTAGCACGGCTCGGTTAACCGGTCGATTAGAGCGGGTAAGTG
AGCAGCCGTTAGTGCTACTCGATGTGGCGCATAATCCCCATGCGGCGCGTTTTTTGGTGCAGCAATTAGCGCCAATGGTT
GTAGGTAAACGCGTATTTGCCCTTTGTGGCATGTTGAAGGATAAAGATATTGCGGGCGTGTTGCCTGTGCTCCATCCTCT
GGTCGACACTTGGTATTTAGTGAGTCTTCATGGGGAGCGTGGCGCGAGTTCTGCCATGCTACGCAAAACCTTAGCACCCG
AGGCCAGCGCATTTGAGTTTGAGGGCATAGCTGAGGCATGGTCGGCATTAAAAGCGCAAATTAACCCAGATGATGTGGTA
ATTGTGTTTGGCTCCTTTTACACTGTGGCAGGTTTTAAGTCTCAATTTATGCAGGAAGTCCGTTTTGTCTAG

Upstream 100 bases:

>100_bases
CAGAACAATATCAATTGCCTAAGCTCGCCCTTGGGCCATTATTCATGTTGGATTGAAAAGATCTTATTTGAGAGATAATC
GAATACGAGGATGTACGGAC

Downstream 100 bases:

>100_bases
CCAGTTTCATAATCGTTTAGTCGGCACTGTGGTGCTGGTTGCGCTTGGAGTGATTTTTTTGCCGGATATTTTGGATGGCA
AAAAAGATCGCCAAGAAGAG

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MLTAPASNAALEQWLDYLLSIHPTEIDMGLTRVSKVAKTLGLLDLGQSKIVTVAGTNGKGTTCAMLENILRLSGLTVGVY
SSPHMLKYNERVRINGSDASDSAFVAAFEQIEAARAEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGRLDATNIIDA
DISVVTSVDLDHESYLGNTRELVGREKAGIFRHGKAAIVGEPNLPHTVNDVANDVGAILYRVGNEFSYQVHGNLWDFQGQ
VLKLHDLALPTLPLPNAATVLAVIEQGWPDIAPAIIAEGISTARLTGRLERVSEQPLVLLDVAHNPHAARFLVQQLAPMV
VGKRVFALCGMLKDKDIAGVLPVLHPLVDTWYLVSLHGERGASSAMLRKTLAPEASAFEFEGIAEAWSALKAQINPDDVV
IVFGSFYTVAGFKSQFMQEVRFV

Sequences:

>Translated_423_residues
MLTAPASNAALEQWLDYLLSIHPTEIDMGLTRVSKVAKTLGLLDLGQSKIVTVAGTNGKGTTCAMLENILRLSGLTVGVY
SSPHMLKYNERVRINGSDASDSAFVAAFEQIEAARAEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGRLDATNIIDA
DISVVTSVDLDHESYLGNTRELVGREKAGIFRHGKAAIVGEPNLPHTVNDVANDVGAILYRVGNEFSYQVHGNLWDFQGQ
VLKLHDLALPTLPLPNAATVLAVIEQGWPDIAPAIIAEGISTARLTGRLERVSEQPLVLLDVAHNPHAARFLVQQLAPMV
VGKRVFALCGMLKDKDIAGVLPVLHPLVDTWYLVSLHGERGASSAMLRKTLAPEASAFEFEGIAEAWSALKAQINPDDVV
IVFGSFYTVAGFKSQFMQEVRFV
>Mature_423_residues
MLTAPASNAALEQWLDYLLSIHPTEIDMGLTRVSKVAKTLGLLDLGQSKIVTVAGTNGKGTTCAMLENILRLSGLTVGVY
SSPHMLKYNERVRINGSDASDSAFVAAFEQIEAARAEISLTFFEYATLAGLILFKAAKLDVVILEVGLGGRLDATNIIDA
DISVVTSVDLDHESYLGNTRELVGREKAGIFRHGKAAIVGEPNLPHTVNDVANDVGAILYRVGNEFSYQVHGNLWDFQGQ
VLKLHDLALPTLPLPNAATVLAVIEQGWPDIAPAIIAEGISTARLTGRLERVSEQPLVLLDVAHNPHAARFLVQQLAPMV
VGKRVFALCGMLKDKDIAGVLPVLHPLVDTWYLVSLHGERGASSAMLRKTLAPEASAFEFEGIAEAWSALKAQINPDDVV
IVFGSFYTVAGFKSQFMQEVRFV

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=187, Percent_Identity=38.5026737967914, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI66932990, Length=187, Percent_Identity=38.5026737967914, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788654, Length=406, Percent_Identity=51.9704433497537, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17553150, Length=174, Percent_Identity=35.632183908046, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71984923, Length=174, Percent_Identity=35.632183908046, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17553148, Length=174, Percent_Identity=35.632183908046, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323760, Length=355, Percent_Identity=29.2957746478873, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324815, Length=181, Percent_Identity=32.0441988950276, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24641571, Length=263, Percent_Identity=31.1787072243346, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24581568, Length=197, Percent_Identity=34.010152284264, Blast_Score=98, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 45663; Mature: 45663

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAPASNAALEQWLDYLLSIHPTEIDMGLTRVSKVAKTLGLLDLGQSKIVTVAGTNGKG
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
TTCAMLENILRLSGLTVGVYSSPHMLKYNERVRINGSDASDSAFVAAFEQIEAARAEISL
CHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHEEE
TFFEYATLAGLILFKAAKLDVVILEVGLGGRLDATNIIDADISVVTSVDLDHESYLGNTR
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCCHHHHCCCHH
ELVGREKAGIFRHGKAAIVGEPNLPHTVNDVANDVGAILYRVGNEFSYQVHGNLWDFQGQ
HHHCCHHCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCC
VLKLHDLALPTLPLPNAATVLAVIEQGWPDIAPAIIAEGISTARLTGRLERVSEQPLVLL
EEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEE
DVAHNPHAARFLVQQLAPMVVGKRVFALCGMLKDKDIAGVLPVLHPLVDTWYLVSLHGER
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCEEEEEEECCC
GASSAMLRKTLAPEASAFEFEGIAEAWSALKAQINPDDVVIVFGSFYTVAGFKSQFMQEV
CCHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHH
RFV
CCC
>Mature Secondary Structure
MLTAPASNAALEQWLDYLLSIHPTEIDMGLTRVSKVAKTLGLLDLGQSKIVTVAGTNGKG
CCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
TTCAMLENILRLSGLTVGVYSSPHMLKYNERVRINGSDASDSAFVAAFEQIEAARAEISL
CHHHHHHHHHHHCCCEEEEECCCCEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHEEE
TFFEYATLAGLILFKAAKLDVVILEVGLGGRLDATNIIDADISVVTSVDLDHESYLGNTR
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCEECCCEEEEEEECCCHHHHCCCHH
ELVGREKAGIFRHGKAAIVGEPNLPHTVNDVANDVGAILYRVGNEFSYQVHGNLWDFQGQ
HHHCCHHCCCEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCCC
VLKLHDLALPTLPLPNAATVLAVIEQGWPDIAPAIIAEGISTARLTGRLERVSEQPLVLL
EEEEEECCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEE
DVAHNPHAARFLVQQLAPMVVGKRVFALCGMLKDKDIAGVLPVLHPLVDTWYLVSLHGER
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCEEEEEEECCC
GASSAMLRKTLAPEASAFEFEGIAEAWSALKAQINPDDVVIVFGSFYTVAGFKSQFMQEV
CCHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHH
RFV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]