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The map label for this gene is xthA [H]

Identifier: 126174923

GI number: 126174923

Start: 3185046

End: 3185858

Strand: Direct

Name: xthA [H]

Synonym: Sbal_2715

Alternate gene names: 126174923

Gene position: 3185046-3185858 (Clockwise)

Preceding gene: 126174922

Following gene: 126174927

Centisome position: 62.12

GC content: 48.22

Gene sequence:

>813_bases
ATGAAAATAGTGTCCTTTAACATCAATGGTCTGCGCGCGCGCTTGCATCAATTACAAGCCTTAATCGACAGCCACCAGCC
CGACATTATCGGCCTGCAAGAAACCAAAGTGCACGACGAAGCGTTTCCATTAGCCGACGTCGAAGCCATGGGATATCAAG
TGCATTACCACGGCGGCAAGGCCCACTATGGCGTTGCTATGTTGTCTAAAGTCGCGCCAGAAAAGCTGCAAAAAGGTTTT
CCAACCGATGACGAAGATGCCCAGCGCCGGATGATTATCGGCACATTTGCCCAAGCGAATGGTCGTCCGTTAACCGTATT
CAACGGCTATTTCCCCCAGGGCGAAAGCATAGACCACCCAACAAAATATCCTGCTAAACGTAAGTTTTATCAGGACTTAA
TGCACTACTTGCAGACAAACCACAGCAACGATGAAGATATCGCCATCATAGGTGATATCAATATTTCGCCGACCGATTTA
GACATAGGTATTGGTGAAGTCAATCGTAAGCGATGGCTGAAAACGGGTAAATGTAGCTTCCAACCGGAAGAGCGTGAGTG
GTTAAAAACCTTGCAAGACTGGGGTTTAGTCGACACTTTCCGTCAATTACACCCAGAGCGCATCGAACGTTATTCTTGGT
TCGATTACCGCAGCAAAGGTTTCGATGACAACCGCGGCCTGCGTATTGATGTGATTTTAGCAACACCTTCACTGGCAGCA
CGTTTAGTCGAATCCGATGTCGATTACGATCTTCGCGGCATAGATAAACCCTCAGACCACGCGCCCATTTGGAGCACGTT
CAAAGAGGCCTAA

Upstream 100 bases:

>100_bases
CCTTAGTGGCAGCAAAACACTGGGCCGATGCCGATCCTTATATCGAAGCGGGCGTGTATAAAAGCGTGGTCGTTAAACCT
TTCAAACGAGTCCTGCCCTA

Downstream 100 bases:

>100_bases
TCCGCAGGGTTAACCATCTCATAGACATAAAAACATCGGCATAAGCCGATGTTTTTGTTTGAGAGTGACTTAAGCAGAAT
GAGATTGGCACAAGTAAGTT

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPLADVEAMGYQVHYHGGKAHYGVAMLSKVAPEKLQKGF
PTDDEDAQRRMIIGTFAQANGRPLTVFNGYFPQGESIDHPTKYPAKRKFYQDLMHYLQTNHSNDEDIAIIGDINISPTDL
DIGIGEVNRKRWLKTGKCSFQPEEREWLKTLQDWGLVDTFRQLHPERIERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
RLVESDVDYDLRGIDKPSDHAPIWSTFKEA

Sequences:

>Translated_270_residues
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPLADVEAMGYQVHYHGGKAHYGVAMLSKVAPEKLQKGF
PTDDEDAQRRMIIGTFAQANGRPLTVFNGYFPQGESIDHPTKYPAKRKFYQDLMHYLQTNHSNDEDIAIIGDINISPTDL
DIGIGEVNRKRWLKTGKCSFQPEEREWLKTLQDWGLVDTFRQLHPERIERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
RLVESDVDYDLRGIDKPSDHAPIWSTFKEA
>Mature_270_residues
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPLADVEAMGYQVHYHGGKAHYGVAMLSKVAPEKLQKGF
PTDDEDAQRRMIIGTFAQANGRPLTVFNGYFPQGESIDHPTKYPAKRKFYQDLMHYLQTNHSNDEDIAIIGDINISPTDL
DIGIGEVNRKRWLKTGKCSFQPEEREWLKTLQDWGLVDTFRQLHPERIERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
RLVESDVDYDLRGIDKPSDHAPIWSTFKEA

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=270, Percent_Identity=26.2962962962963, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI18375503, Length=270, Percent_Identity=26.2962962962963, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI18375501, Length=270, Percent_Identity=26.2962962962963, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=63.8059701492537, Blast_Score=376, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71989536, Length=271, Percent_Identity=26.5682656826568, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI221330655, Length=269, Percent_Identity=30.4832713754647, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136678, Length=269, Percent_Identity=30.4832713754647, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30966; Mature: 30966

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPLADVEAMGYQVHYHGGK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEECCHHCCCCCCCCCCCCHHHHCEEEEEECCC
AHYGVAMLSKVAPEKLQKGFPTDDEDAQRRMIIGTFAQANGRPLTVFNGYFPQGESIDHP
HHHHHHHHHHHCHHHHHCCCCCCCHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
TKYPAKRKFYQDLMHYLQTNHSNDEDIAIIGDINISPTDLDIGIGEVNRKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCC
QPEEREWLKTLQDWGLVDTFRQLHPERIERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
CCHHHHHHHHHHHCCHHHHHHHHCHHHHHHHCCCCHHCCCCCCCCCCEEEEEEECHHHHH
RLVESDVDYDLRGIDKPSDHAPIWSTFKEA
HHHHCCCCCCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MKIVSFNINGLRARLHQLQALIDSHQPDIIGLQETKVHDEAFPLADVEAMGYQVHYHGGK
CEEEEECCHHHHHHHHHHHHHHHCCCCCEECCHHCCCCCCCCCCCCHHHHCEEEEEECCC
AHYGVAMLSKVAPEKLQKGFPTDDEDAQRRMIIGTFAQANGRPLTVFNGYFPQGESIDHP
HHHHHHHHHHHCHHHHHCCCCCCCHHHHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
TKYPAKRKFYQDLMHYLQTNHSNDEDIAIIGDINISPTDLDIGIGEVNRKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCC
QPEEREWLKTLQDWGLVDTFRQLHPERIERYSWFDYRSKGFDDNRGLRIDVILATPSLAA
CCHHHHHHHHHHHCCHHHHHHHHCHHHHHHHCCCCHHCCCCCCCCCCEEEEEEECHHHHH
RLVESDVDYDLRGIDKPSDHAPIWSTFKEA
HHHHCCCCCCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]