Click here to switch to the map view.

The map label for this gene is trpA

Identifier: 126174914

GI number: 126174914

Start: 3173743

End: 3174564

Strand: Direct

Name: trpA

Synonym: Sbal_2706

Alternate gene names: 126174914

Gene position: 3173743-3174564 (Clockwise)

Preceding gene: 126174913

Following gene: 126174915

Centisome position: 61.9

GC content: 53.04

Gene sequence:

>822_bases
ATGAGTGATACATTGACTAACACTGGCCGTTACCAAGCCACCTTTGCCAAACTCAAAGCCGAAGGCCGTGGTGCTTTTGT
CCCATTCGTCACCCTTGGCGACCCTAGCCCTGAGTTATCGCTGAAAATTATCGATACCTTAGTGCAAAACGGTGCAGATG
CCTTAGAACTGGGCTTTCCGTTTTCCGATCCCTTGGCCGATGGCCCTGTGATCCAAGGTGCTAACCTGCGTTCTTTAGCT
GCGGGCACGACTCCAACGGCGTGTTTTGAATTACTGGCGCAAGTGCGTGCTAAATATCCTGAACTGCCAATCGGTTTGCT
GCTGTATGCGAACTTGGTGTTTGCTAATGGCATAGATGCGTTTTACGCCAAAGCGCAGCAAGCGGGTGTGGATTCAGTGC
TTATTGCCGACGTACCCGTGGAAGAATCGGCGCCCTTTAGCGAAGCGGCTAAAGCCCATGGTATTGCGCCAATTTTTATC
GCGCCGCCCAATGCCGACAGTGAAACCCTTGCACTGGTTAGCGCCAGCGGTGAAGGTTACACCTATCTCTTATCCCGCGC
AGGGGTGACAGGCACAGACACTAAAGCTGGCGTTCCGGTGGAAGAAATTCTATCCAAGTTGAAAACTTTTAACGCGCCGC
CACCGCTACTCGGCTTTGGCATTGCCGAACCCGCTCAAGTGAGCGCCGCAATCAAAGCAGGCGCTGCAGGGGCAATTTCA
GGCTCTGCTGTCGTGAAAATCATCGAAACCCACCAGCACGATGAAGCTAAGCTACTGGCGACACTTGGCGATTTTACTCG
CGCCATGAAAGCGGCGACCTAA

Upstream 100 bases:

>100_bases
GCACCAAAGAAACCATTTTAGTGGTCAATCTTTCCGGTCGTGGCGACAAAGATATTTTCACCGTTTCTGACATTTTGAAT
GGTAAAGAGGTATAACGAAC

Downstream 100 bases:

>100_bases
GCGAGTCTTGATTTAAATAATCCAATATAAATCAAGAATTCAATTACAAATAAAATAAGTGAGGTAGACTCGTCGCCTCG
CTTATTTTGTTTATATTTAC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSDTLTNTGRYQATFAKLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADALELGFPFSDPLADGPVIQGANLRSLA
AGTTPTACFELLAQVRAKYPELPIGLLLYANLVFANGIDAFYAKAQQAGVDSVLIADVPVEESAPFSEAAKAHGIAPIFI
APPNADSETLALVSASGEGYTYLLSRAGVTGTDTKAGVPVEEILSKLKTFNAPPPLLGFGIAEPAQVSAAIKAGAAGAIS
GSAVVKIIETHQHDEAKLLATLGDFTRAMKAAT

Sequences:

>Translated_273_residues
MSDTLTNTGRYQATFAKLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADALELGFPFSDPLADGPVIQGANLRSLA
AGTTPTACFELLAQVRAKYPELPIGLLLYANLVFANGIDAFYAKAQQAGVDSVLIADVPVEESAPFSEAAKAHGIAPIFI
APPNADSETLALVSASGEGYTYLLSRAGVTGTDTKAGVPVEEILSKLKTFNAPPPLLGFGIAEPAQVSAAIKAGAAGAIS
GSAVVKIIETHQHDEAKLLATLGDFTRAMKAAT
>Mature_272_residues
SDTLTNTGRYQATFAKLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADALELGFPFSDPLADGPVIQGANLRSLAA
GTTPTACFELLAQVRAKYPELPIGLLLYANLVFANGIDAFYAKAQQAGVDSVLIADVPVEESAPFSEAAKAHGIAPIFIA
PPNADSETLALVSASGEGYTYLLSRAGVTGTDTKAGVPVEEILSKLKTFNAPPPLLGFGIAEPAQVSAAIKAGAAGAISG
SAVVKIIETHQHDEAKLLATLGDFTRAMKAAT

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=264, Percent_Identity=65.1515151515152, Blast_Score=336, Evalue=1e-93,
Organism=Saccharomyces cerevisiae, GI6321412, Length=251, Percent_Identity=31.8725099601594, Blast_Score=135, Evalue=5e-33,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_SHEB5 (A3D631)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001051063.1
- ProteinModelPortal:   A3D631
- SMR:   A3D631
- STRING:   A3D631
- GeneID:   4843379
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_2706
- NMPDR:   fig|325240.3.peg.1878
- eggNOG:   COG0159
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- PhylomeDB:   A3D631
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28123; Mature: 27992

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 56-56 ACT_SITE 67-67

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTLTNTGRYQATFAKLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADALELGFP
CCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCC
FSDPLADGPVIQGANLRSLAAGTTPTACFELLAQVRAKYPELPIGLLLYANLVFANGIDA
CCCCCCCCCEECCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHH
FYAKAQQAGVDSVLIADVPVEESAPFSEAAKAHGIAPIFIAPPNADSETLALVSASGEGY
HHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCE
TYLLSRAGVTGTDTKAGVPVEEILSKLKTFNAPPPLLGFGIAEPAQVSAAIKAGAAGAIS
EEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC
GSAVVKIIETHQHDEAKLLATLGDFTRAMKAAT
CHHHEEEEHHCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDTLTNTGRYQATFAKLKAEGRGAFVPFVTLGDPSPELSLKIIDTLVQNGADALELGFP
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCC
FSDPLADGPVIQGANLRSLAAGTTPTACFELLAQVRAKYPELPIGLLLYANLVFANGIDA
CCCCCCCCCEECCCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHH
FYAKAQQAGVDSVLIADVPVEESAPFSEAAKAHGIAPIFIAPPNADSETLALVSASGEGY
HHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCE
TYLLSRAGVTGTDTKAGVPVEEILSKLKTFNAPPPLLGFGIAEPAQVSAAIKAGAAGAIS
EEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEECCCCCHHHHHHHHHCCCCCCCC
GSAVVKIIETHQHDEAKLLATLGDFTRAMKAAT
CHHHEEEEHHCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA